Particle-loaded hollow-fiber membrane adsorbers for lysozyme separation

M.E. Avramescu, Zandrie Borneman, Matthias Wessling

Research output: Contribution to journalArticleAcademicpeer-review

43 Citations (Scopus)

Abstract

The separation of lysozyme (LZ), a valuable enzyme naturally present in chicken egg white, was carried out using a new type of ion exchange hollow-fiber membranes. Functionalities were incorporated into the polymeric membranes by dispersing ion-exchange resins (IERs) in a microporous structure formed by phase inversion. The obtained hollow-fibers were composed of ion-exchange particles surrounded by a polymeric matrix and possessed both high static and dynamic adsorption capacities of more than 60 mg/ml membrane. The hollow-fiber membrane adsorbers were connected in series with different numbers of fibers thereby increasing the effective thickness and the protein residence time within the module. By choosing appropriate operation conditions, the membranes adsorbed solely LZ from fresh chicken egg white (eventually also the minor component avidin), whereas the adsorption of ovalbumin, ovotransferrin, and other low isoelectric point proteins was negligible. An average separation factor for LZ of about 150 was calculated by numerical integration of the protein concentrations in the elution curve during the filtration run. The effect of the filtration flow rate, protein concentration and ionic strength on the membrane's performance was investigated to determine the optimum operation parameters.
Original languageUndefined
Pages (from-to)306-313
JournalJournal of membrane science
Volume322
Issue number2
DOIs
Publication statusPublished - 2008

Keywords

  • METIS-250816
  • IR-71535

Cite this