Particle size determination in impedance flow cytometry using measured opacity

Koen F.A. Jorissen, Wouter Olthuis, Albert van den Berg

Research output: Contribution to conferencePosterAcademic

Abstract

Microfluidic electrical flow cytometry is a popular method to study a wide variety of biological cell properties. Unfortunately, when using coplanar electrodes, this method is sensitive to positional differences of passing particles or cells.
In this work we present a novel compensation method to account for the particle position in a coplanar electrode setup using the measured electrical opacity.
We demonstrate an accurate size discrimination of 5, 6 and 7 µm polystyrene beads irrespective of their position using the measured electrical opacity making use of the variation of electrical field strength with height in the channel. Thus, only two electrodes are required, which is favorable for microfluidic devices with size limitations.
Original languageEnglish
Publication statusPublished - 2021
Event21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021 - Virtual, Online, United States
Duration: 20 Jun 202125 Jun 2021
Conference number: 21

Conference

Conference21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021
Abbreviated titleTransducers 2021
CountryUnited States
CityVirtual, Online
Period20/06/2125/06/21

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