Particle Size Determination in Impedance Flow Cytometry Using Measured Opacity

Douwe S. De Bruijn; Koen F.A. Jorissen; Wouter Olthuis; Albert van den Berg

doi:10.1109/Transducers50396.2021.9495740

Particle Size Determination in Impedance Flow Cytometry Using Measured Opacity

Douwe S. De Bruijn, Koen F.A. Jorissen, Wouter Olthuis, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

3 Citations (Scopus)

216 Downloads (Pure)

Abstract

Microfluidic electrical flow cytometry is a popular method to study a wide variety of biological cell properties. Unfortunately, when using coplanar electrodes, this method is sensitive to positional differences of passing particles or cells. In this work we present a novel compensation method to account for the particle position in a coplanar electrode setup using the measured electrical opacity. We demonstrate an accurate size discrimination of 5, 6 and 7 m polystyrene beads irrespective of their position using the measured electrical opacity making use of the variation of electrical field strength with height in the channel. Thus, only two electrodes are required, which is favorable for microfluidic devices with size limitations.

Original language	English
Title of host publication	21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021
Place of Publication	Piscataway, NJ
Publisher	IEEE
Pages	1036-1039
Number of pages	4
ISBN (Electronic)	978-1-6654-1267-4
ISBN (Print)	978-1-6654-4845-1
DOIs	https://doi.org/10.1109/Transducers50396.2021.9495740
Publication status	Published - 20 Jun 2021
Event	21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021 - Virtual, Online, United States Duration: 20 Jun 2021 → 25 Jun 2021 Conference number: 21

Publication series

Name	International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)
Publisher	IEEE
Volume	2021
ISSN (Print)	2167-0013
ISSN (Electronic)	2167-0021

Conference

Conference	21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021
Abbreviated title	Transducers 2021
Country/Territory	United States
City	Virtual, Online
Period	20/06/21 → 25/06/21

Keywords

2022 OA procedure
Electrical opacity
Flow cytometry
Microfluidics
Size determination
Coplanar electrodes

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10.1109/Transducers50396.2021.9495740

DeBruijn2021particleFinal published version, 875 KBLicence: Taverne

Cite this

De Bruijn, D. S., Jorissen, K. F. A., Olthuis, W., & van den Berg, A. (2021). Particle Size Determination in Impedance Flow Cytometry Using Measured Opacity. In 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021 (pp. 1036-1039). (International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS); Vol. 2021). IEEE. https://doi.org/10.1109/Transducers50396.2021.9495740

De Bruijn, Douwe S. ; Jorissen, Koen F.A. ; Olthuis, Wouter et al. / Particle Size Determination in Impedance Flow Cytometry Using Measured Opacity. 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021. Piscataway, NJ : IEEE, 2021. pp. 1036-1039 (International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)).

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title = "Particle Size Determination in Impedance Flow Cytometry Using Measured Opacity",

abstract = "Microfluidic electrical flow cytometry is a popular method to study a wide variety of biological cell properties. Unfortunately, when using coplanar electrodes, this method is sensitive to positional differences of passing particles or cells. In this work we present a novel compensation method to account for the particle position in a coplanar electrode setup using the measured electrical opacity. We demonstrate an accurate size discrimination of 5, 6 and 7 m polystyrene beads irrespective of their position using the measured electrical opacity making use of the variation of electrical field strength with height in the channel. Thus, only two electrodes are required, which is favorable for microfluidic devices with size limitations.",

keywords = "2022 OA procedure, Electrical opacity, Flow cytometry, Microfluidics, Size determination, Coplanar electrodes",

author = "{De Bruijn}, {Douwe S.} and Jorissen, {Koen F.A.} and Wouter Olthuis and {van den Berg}, Albert",

note = "Funding Information: This work is part of the research programme of the Foundation for Fundamental Research on Matter (FOM), which is part of the Dutch Research Council (NWO). We would like to thank the Max Planck – Center for Complex Fluid Dynamics for support of this project. Funding Information: We would like to thank the Max Planck - Center for Complex Fluid Dynamics for support of this project. Publisher Copyright: {\textcopyright} 2021 IEEE.; 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021, Transducers 2021 ; Conference date: 20-06-2021 Through 25-06-2021",

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doi = "10.1109/Transducers50396.2021.9495740",

language = "English",

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De Bruijn, DS, Jorissen, KFA, Olthuis, W & van den Berg, A 2021, Particle Size Determination in Impedance Flow Cytometry Using Measured Opacity. in 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021. International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), vol. 2021, IEEE, Piscataway, NJ, pp. 1036-1039, 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021, Virtual, Online, United States, 20/06/21. https://doi.org/10.1109/Transducers50396.2021.9495740

Particle Size Determination in Impedance Flow Cytometry Using Measured Opacity. / De Bruijn, Douwe S.; Jorissen, Koen F.A.; Olthuis, Wouter et al.
21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021. Piscataway, NJ: IEEE, 2021. p. 1036-1039 (International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS); Vol. 2021).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Particle Size Determination in Impedance Flow Cytometry Using Measured Opacity

AU - De Bruijn, Douwe S.

AU - Jorissen, Koen F.A.

AU - Olthuis, Wouter

AU - van den Berg, Albert

N1 - Conference code: 21

PY - 2021/6/20

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N2 - Microfluidic electrical flow cytometry is a popular method to study a wide variety of biological cell properties. Unfortunately, when using coplanar electrodes, this method is sensitive to positional differences of passing particles or cells. In this work we present a novel compensation method to account for the particle position in a coplanar electrode setup using the measured electrical opacity. We demonstrate an accurate size discrimination of 5, 6 and 7 m polystyrene beads irrespective of their position using the measured electrical opacity making use of the variation of electrical field strength with height in the channel. Thus, only two electrodes are required, which is favorable for microfluidic devices with size limitations.

AB - Microfluidic electrical flow cytometry is a popular method to study a wide variety of biological cell properties. Unfortunately, when using coplanar electrodes, this method is sensitive to positional differences of passing particles or cells. In this work we present a novel compensation method to account for the particle position in a coplanar electrode setup using the measured electrical opacity. We demonstrate an accurate size discrimination of 5, 6 and 7 m polystyrene beads irrespective of their position using the measured electrical opacity making use of the variation of electrical field strength with height in the channel. Thus, only two electrodes are required, which is favorable for microfluidic devices with size limitations.

KW - 2022 OA procedure

KW - Electrical opacity

KW - Flow cytometry

KW - Microfluidics

KW - Size determination

KW - Coplanar electrodes

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DO - 10.1109/Transducers50396.2021.9495740

M3 - Conference contribution

AN - SCOPUS:85114964911

SN - 978-1-6654-4845-1

T3 - International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)

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EP - 1039

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PB - IEEE

CY - Piscataway, NJ

T2 - 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021

Y2 - 20 June 2021 through 25 June 2021

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De Bruijn DS, Jorissen KFA, Olthuis W, van den Berg A. Particle Size Determination in Impedance Flow Cytometry Using Measured Opacity. In 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021. Piscataway, NJ: IEEE. 2021. p. 1036-1039. (International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)). doi: 10.1109/Transducers50396.2021.9495740

Particle Size Determination in Impedance Flow Cytometry Using Measured Opacity

Abstract

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Keywords

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