Pattern Formation by Staphylococcus epidermidis via Droplet Evaporation on Micropillars Arrays at a Surface

A. Susarrey Arce, Alvaro Gomez Marin, A. Massey, A. Oknianska, Y. Diaz-Fernandez, J.F. Hernandez Sanchez, E. Griffiths, Johannes G.E. Gardeniers, Jacobus Hendrikus Snoeijer, Detlef Lohse, R. Raval

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

We evaluate the effect of epoxy surface structuring on the evaporation of water droplets containing Staphylococcus epidermidis (S. epidermidis). During evaporation, droplets with S. epidermidis cells yield to complex wetting patterns such as the zipping-wetting1−3 and the coffee-stain effects. Depending on the height of the microstructure, the wetting fronts propagate circularly or in a stepwise manner, leading to the formation of octagonal or square-shaped deposition patterns.4,5 We observed that the shape of the dried droplets has considerable influence on the local spatial distribution of S. epidermidis deposited between micropillars. These changes are attributed to an unexplored interplay between the zipping-wetting1 and the coffee-stain6 effects in polygonally shaped droplets containing S. epidermidis. Induced capillary flows during evaporation of S. epidermidis are modeled with polystyrene particles. Bacterial viability measurements for S. epidermidis show high viability of planktonic cells, but low biomass deposition on the microstructured surfaces. Our findings provide insights into design criteria for the development of microstructured surfaces on which bacterial propagation could be controlled, limiting the use of biocides.
Original languageEnglish
Pages (from-to)7159-7169
Number of pages11
JournalLangmuir
Volume32
Issue number28
DOIs
Publication statusPublished - 2016

Fingerprint

staphylococcus
Evaporation
evaporation
Coffee
coffee
Wetting
Capillary flow
viability
Biocides
wetting
Disinfectants
Polystyrenes
Spatial distribution
capillary flow
Biomass
Coloring Agents
Cells
biomass
cells
Microstructure

Keywords

  • IR-101180
  • METIS-317372

Cite this

Susarrey Arce, A. ; Gomez Marin, Alvaro ; Massey, A. ; Oknianska, A. ; Diaz-Fernandez, Y. ; Hernandez Sanchez, J.F. ; Griffiths, E. ; Gardeniers, Johannes G.E. ; Snoeijer, Jacobus Hendrikus ; Lohse, Detlef ; Raval, R. / Pattern Formation by Staphylococcus epidermidis via Droplet Evaporation on Micropillars Arrays at a Surface. In: Langmuir. 2016 ; Vol. 32, No. 28. pp. 7159-7169.
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title = "Pattern Formation by Staphylococcus epidermidis via Droplet Evaporation on Micropillars Arrays at a Surface",
abstract = "We evaluate the effect of epoxy surface structuring on the evaporation of water droplets containing Staphylococcus epidermidis (S. epidermidis). During evaporation, droplets with S. epidermidis cells yield to complex wetting patterns such as the zipping-wetting1−3 and the coffee-stain effects. Depending on the height of the microstructure, the wetting fronts propagate circularly or in a stepwise manner, leading to the formation of octagonal or square-shaped deposition patterns.4,5 We observed that the shape of the dried droplets has considerable influence on the local spatial distribution of S. epidermidis deposited between micropillars. These changes are attributed to an unexplored interplay between the zipping-wetting1 and the coffee-stain6 effects in polygonally shaped droplets containing S. epidermidis. Induced capillary flows during evaporation of S. epidermidis are modeled with polystyrene particles. Bacterial viability measurements for S. epidermidis show high viability of planktonic cells, but low biomass deposition on the microstructured surfaces. Our findings provide insights into design criteria for the development of microstructured surfaces on which bacterial propagation could be controlled, limiting the use of biocides.",
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Pattern Formation by Staphylococcus epidermidis via Droplet Evaporation on Micropillars Arrays at a Surface. / Susarrey Arce, A.; Gomez Marin, Alvaro; Massey, A.; Oknianska, A.; Diaz-Fernandez, Y.; Hernandez Sanchez, J.F.; Griffiths, E.; Gardeniers, Johannes G.E.; Snoeijer, Jacobus Hendrikus; Lohse, Detlef; Raval, R.

In: Langmuir, Vol. 32, No. 28, 2016, p. 7159-7169.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Pattern Formation by Staphylococcus epidermidis via Droplet Evaporation on Micropillars Arrays at a Surface

AU - Susarrey Arce, A.

AU - Gomez Marin, Alvaro

AU - Massey, A.

AU - Oknianska, A.

AU - Diaz-Fernandez, Y.

AU - Hernandez Sanchez, J.F.

AU - Griffiths, E.

AU - Gardeniers, Johannes G.E.

AU - Snoeijer, Jacobus Hendrikus

AU - Lohse, Detlef

AU - Raval, R.

PY - 2016

Y1 - 2016

N2 - We evaluate the effect of epoxy surface structuring on the evaporation of water droplets containing Staphylococcus epidermidis (S. epidermidis). During evaporation, droplets with S. epidermidis cells yield to complex wetting patterns such as the zipping-wetting1−3 and the coffee-stain effects. Depending on the height of the microstructure, the wetting fronts propagate circularly or in a stepwise manner, leading to the formation of octagonal or square-shaped deposition patterns.4,5 We observed that the shape of the dried droplets has considerable influence on the local spatial distribution of S. epidermidis deposited between micropillars. These changes are attributed to an unexplored interplay between the zipping-wetting1 and the coffee-stain6 effects in polygonally shaped droplets containing S. epidermidis. Induced capillary flows during evaporation of S. epidermidis are modeled with polystyrene particles. Bacterial viability measurements for S. epidermidis show high viability of planktonic cells, but low biomass deposition on the microstructured surfaces. Our findings provide insights into design criteria for the development of microstructured surfaces on which bacterial propagation could be controlled, limiting the use of biocides.

AB - We evaluate the effect of epoxy surface structuring on the evaporation of water droplets containing Staphylococcus epidermidis (S. epidermidis). During evaporation, droplets with S. epidermidis cells yield to complex wetting patterns such as the zipping-wetting1−3 and the coffee-stain effects. Depending on the height of the microstructure, the wetting fronts propagate circularly or in a stepwise manner, leading to the formation of octagonal or square-shaped deposition patterns.4,5 We observed that the shape of the dried droplets has considerable influence on the local spatial distribution of S. epidermidis deposited between micropillars. These changes are attributed to an unexplored interplay between the zipping-wetting1 and the coffee-stain6 effects in polygonally shaped droplets containing S. epidermidis. Induced capillary flows during evaporation of S. epidermidis are modeled with polystyrene particles. Bacterial viability measurements for S. epidermidis show high viability of planktonic cells, but low biomass deposition on the microstructured surfaces. Our findings provide insights into design criteria for the development of microstructured surfaces on which bacterial propagation could be controlled, limiting the use of biocides.

KW - IR-101180

KW - METIS-317372

U2 - 10.1021/acs.langmuir.6b01658

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