Patterning of peptide nucleic acids using reactive microcontact printing

Alessandro Calabretta, D. Wasserberg, Geertruida A. Posthuma-Trumpie, Vinod Subramaniam, Aart van Amerongen, Roberto Corradini, Jurriaan Huskens, Tullia Tedeschi, Stefano Sforza, Pascal Jonkheijm, David Reinhoudt, Rosangela Marchelli

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Scopus)
3 Downloads (Pure)

Abstract

PNAs (peptide nucleic acids) have been immobilized onto surfaces in a fast, accurate way by employing reactive microcontact printing. Surfaces have been first modified with aldehyde groups to react with the amino end of the synthesized PNAs. When patterning fluorescein-labeled PNAs by reactive microcontact printing using oxygen-oxidized polydimethylsiloxane stamps, homogeneous arrays were fabricated and characterized using optical methods. PNA-patterned surfaces were hybridized with complementary and mismatched dye-labeled oligonucleotides to test their ability to recognize DNA sequences. The stability and selectivity of the PNA-DNA duplexes on surfaces have been verified by fluorescence microscopy, and the melting curves have been recorded. Finally, the technique has been applied to the fabrication of chips by spotting a PNA microarray onto a flat PDMS stamp and reproducing the same features onto many slides. The chips were finally applied to single nucleotide polymorphism detection on oligonucleotides.
Original languageEnglish
Pages (from-to)1536-1542
Number of pages7
JournalLangmuir
Volume27
Issue number4
DOIs
Publication statusPublished - 2011

Keywords

  • METIS-282159
  • IR-76903

Fingerprint Dive into the research topics of 'Patterning of peptide nucleic acids using reactive microcontact printing'. Together they form a unique fingerprint.

Cite this