Patterns of amino Acid metabolism by proliferating human mesenchymal stem cells

Gustavo A. Higuera, Deborah Schop, Tim W.G.M. Spitters, Riemke van Dijkhuizen-Radersma, Madelon Bracke, Joost D. de Bruijn, Dirk Martens, Marcel Karperien, Anton van Boxtel, Clemens van Blitterswijk

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

The nutritional requirements of stem cells have not been determined; in particular, the amino acid metabolism of stem cells is largely unknown. In this study, we investigated the amino acid metabolism of human mesenchymal stem cells (hMSCs), with focus on two questions: Which amino acids are consumed and/or secreted by hMSCs and at what rates? To answer these questions, hMSCs were cultured on tissue culture plastic and in a bioreactor, and their amino acid profile was analyzed. The results showed that the kinetics of hMSCs growth and amino acid metabolism were significantly higher for hMSCs in tissue culture plastic than in the bioreactor. Despite differences in culture conditions, 8 essential and 6 nonessential amino acids were consumed by hMSCs in both tissue culture plastic and bioreactor cultures. Glutamine was the most consumed amino acid with significantly higher rates than for any other amino acid. The metabolism of nonessential amino acids by hMSCs deviated significantly from that of other cell lines. The secretion of alanine, glycine, glutamate, and ornithine by hMSCs showed that there is a strong overflow metabolism that can be due to the high concentrations of amino acids provided in the medium. In addition, the data showed that there is a metabolic pattern for proliferating hMSCs, which can contribute to the design of medium without animal serum for stem cells. Further, this study shows how to implement amino acid rates and metabolic principles in three-dimensional stem cell biology.
Original languageEnglish
Pages (from-to)654-664
JournalTissue engineering. Part A
Volume18
Issue number5-6
DOIs
Publication statusPublished - 2012

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Stem cells
Mesenchymal Stromal Cells
Metabolism
Amino acids
Amino Acids
Bioreactors
Tissue culture
Stem Cells
Plastics
Nutritional Requirements
Cytology
Ornithine
Enzyme kinetics
Glutamine
Alanine
Glycine
Cell growth
Cell Biology
Glutamic Acid
Cell culture

Keywords

  • IR-80570
  • METIS-286523

Cite this

Higuera, G. A., Schop, D., Spitters, T. W. G. M., van Dijkhuizen-Radersma, R., Bracke, M., de Bruijn, J. D., ... van Blitterswijk, C. (2012). Patterns of amino Acid metabolism by proliferating human mesenchymal stem cells. Tissue engineering. Part A, 18(5-6), 654-664. https://doi.org/10.1089/ten.tea.2011.0223
Higuera, Gustavo A. ; Schop, Deborah ; Spitters, Tim W.G.M. ; van Dijkhuizen-Radersma, Riemke ; Bracke, Madelon ; de Bruijn, Joost D. ; Martens, Dirk ; Karperien, Marcel ; van Boxtel, Anton ; van Blitterswijk, Clemens. / Patterns of amino Acid metabolism by proliferating human mesenchymal stem cells. In: Tissue engineering. Part A. 2012 ; Vol. 18, No. 5-6. pp. 654-664.
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abstract = "The nutritional requirements of stem cells have not been determined; in particular, the amino acid metabolism of stem cells is largely unknown. In this study, we investigated the amino acid metabolism of human mesenchymal stem cells (hMSCs), with focus on two questions: Which amino acids are consumed and/or secreted by hMSCs and at what rates? To answer these questions, hMSCs were cultured on tissue culture plastic and in a bioreactor, and their amino acid profile was analyzed. The results showed that the kinetics of hMSCs growth and amino acid metabolism were significantly higher for hMSCs in tissue culture plastic than in the bioreactor. Despite differences in culture conditions, 8 essential and 6 nonessential amino acids were consumed by hMSCs in both tissue culture plastic and bioreactor cultures. Glutamine was the most consumed amino acid with significantly higher rates than for any other amino acid. The metabolism of nonessential amino acids by hMSCs deviated significantly from that of other cell lines. The secretion of alanine, glycine, glutamate, and ornithine by hMSCs showed that there is a strong overflow metabolism that can be due to the high concentrations of amino acids provided in the medium. In addition, the data showed that there is a metabolic pattern for proliferating hMSCs, which can contribute to the design of medium without animal serum for stem cells. Further, this study shows how to implement amino acid rates and metabolic principles in three-dimensional stem cell biology.",
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author = "Higuera, {Gustavo A.} and Deborah Schop and Spitters, {Tim W.G.M.} and {van Dijkhuizen-Radersma}, Riemke and Madelon Bracke and {de Bruijn}, {Joost D.} and Dirk Martens and Marcel Karperien and {van Boxtel}, Anton and {van Blitterswijk}, Clemens",
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Higuera, GA, Schop, D, Spitters, TWGM, van Dijkhuizen-Radersma, R, Bracke, M, de Bruijn, JD, Martens, D, Karperien, M, van Boxtel, A & van Blitterswijk, C 2012, 'Patterns of amino Acid metabolism by proliferating human mesenchymal stem cells' Tissue engineering. Part A, vol. 18, no. 5-6, pp. 654-664. https://doi.org/10.1089/ten.tea.2011.0223

Patterns of amino Acid metabolism by proliferating human mesenchymal stem cells. / Higuera, Gustavo A.; Schop, Deborah; Spitters, Tim W.G.M.; van Dijkhuizen-Radersma, Riemke; Bracke, Madelon; de Bruijn, Joost D.; Martens, Dirk; Karperien, Marcel; van Boxtel, Anton; van Blitterswijk, Clemens.

In: Tissue engineering. Part A, Vol. 18, No. 5-6, 2012, p. 654-664.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Patterns of amino Acid metabolism by proliferating human mesenchymal stem cells

AU - Higuera, Gustavo A.

AU - Schop, Deborah

AU - Spitters, Tim W.G.M.

AU - van Dijkhuizen-Radersma, Riemke

AU - Bracke, Madelon

AU - de Bruijn, Joost D.

AU - Martens, Dirk

AU - Karperien, Marcel

AU - van Boxtel, Anton

AU - van Blitterswijk, Clemens

PY - 2012

Y1 - 2012

N2 - The nutritional requirements of stem cells have not been determined; in particular, the amino acid metabolism of stem cells is largely unknown. In this study, we investigated the amino acid metabolism of human mesenchymal stem cells (hMSCs), with focus on two questions: Which amino acids are consumed and/or secreted by hMSCs and at what rates? To answer these questions, hMSCs were cultured on tissue culture plastic and in a bioreactor, and their amino acid profile was analyzed. The results showed that the kinetics of hMSCs growth and amino acid metabolism were significantly higher for hMSCs in tissue culture plastic than in the bioreactor. Despite differences in culture conditions, 8 essential and 6 nonessential amino acids were consumed by hMSCs in both tissue culture plastic and bioreactor cultures. Glutamine was the most consumed amino acid with significantly higher rates than for any other amino acid. The metabolism of nonessential amino acids by hMSCs deviated significantly from that of other cell lines. The secretion of alanine, glycine, glutamate, and ornithine by hMSCs showed that there is a strong overflow metabolism that can be due to the high concentrations of amino acids provided in the medium. In addition, the data showed that there is a metabolic pattern for proliferating hMSCs, which can contribute to the design of medium without animal serum for stem cells. Further, this study shows how to implement amino acid rates and metabolic principles in three-dimensional stem cell biology.

AB - The nutritional requirements of stem cells have not been determined; in particular, the amino acid metabolism of stem cells is largely unknown. In this study, we investigated the amino acid metabolism of human mesenchymal stem cells (hMSCs), with focus on two questions: Which amino acids are consumed and/or secreted by hMSCs and at what rates? To answer these questions, hMSCs were cultured on tissue culture plastic and in a bioreactor, and their amino acid profile was analyzed. The results showed that the kinetics of hMSCs growth and amino acid metabolism were significantly higher for hMSCs in tissue culture plastic than in the bioreactor. Despite differences in culture conditions, 8 essential and 6 nonessential amino acids were consumed by hMSCs in both tissue culture plastic and bioreactor cultures. Glutamine was the most consumed amino acid with significantly higher rates than for any other amino acid. The metabolism of nonessential amino acids by hMSCs deviated significantly from that of other cell lines. The secretion of alanine, glycine, glutamate, and ornithine by hMSCs showed that there is a strong overflow metabolism that can be due to the high concentrations of amino acids provided in the medium. In addition, the data showed that there is a metabolic pattern for proliferating hMSCs, which can contribute to the design of medium without animal serum for stem cells. Further, this study shows how to implement amino acid rates and metabolic principles in three-dimensional stem cell biology.

KW - IR-80570

KW - METIS-286523

U2 - 10.1089/ten.tea.2011.0223

DO - 10.1089/ten.tea.2011.0223

M3 - Article

VL - 18

SP - 654

EP - 664

JO - Tissue engineering. Part A

JF - Tissue engineering. Part A

SN - 1937-3341

IS - 5-6

ER -

Higuera GA, Schop D, Spitters TWGM, van Dijkhuizen-Radersma R, Bracke M, de Bruijn JD et al. Patterns of amino Acid metabolism by proliferating human mesenchymal stem cells. Tissue engineering. Part A. 2012;18(5-6):654-664. https://doi.org/10.1089/ten.tea.2011.0223