Pectin Methacrylate (PEMA) and Gelatin-Based Hydrogels for Cell Delivery: Converting Waste Materials into Biomaterials

Mehdi Mehrali, Ashish Thakur, Firoz Babu Kadumudi, Malgorzata Karolina Pierchala, Julio Alvin Vacacela Cordova, Mohammad-Ali Shahbazi, Mohammad Mehrali, Cristian Pablo Pennisi, Gorka Orive, Akhilesh K. Gaharwar, Alireza Dolatshahi-Pirouz*

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    63 Citations (Scopus)
    96 Downloads (Pure)


    The emergence of nontoxic, eco-friendly, and biocompatible polymers derived from natural sources has added a new and exciting dimension to the development of low-cost and scalable biomaterials for tissue engineering applications. Here, we have developed a mechanically strong and durable hydrogel composed of an eco-friendly biopolymer that exists within the cell walls of fruits and plants. Its trade name is pectin, and it bears many similarities with natural polysaccharides in the native extracellular matrix. Specifically, we have employed a new pathway to transform pectin into a ultraviolet (UV)-cross-linkable pectin methacrylate (PEMA) polymer. To endow this hydrogel matrix with cell differentiation and cell spreading properties, we have also incorporated thiolated gelatin into the system. Notably, we were able to fine-tune the compressive modulus of this hydrogel in the range ∼0.5 to ∼24 kPa: advantageously, our results demonstrated that the hydrogels can support growth and viability for a wide range of three-dimensionally (3D) encapsulated cells that include muscle progenitor (C2C12), neural progenitor (PC12), and human mesenchymal stem cells (hMSCs). Our results also indicate that PEMA-gelatin-encapsulated hMSCs can facilitate the formation of bonelike apatite after 5 weeks in culture. Finally, we have demonstrated that PEMA-gelatin can yield micropatterned cell-laden 3D constructs through UV light-assisted lithography. The simplicity, scalability, processability, tunability, bioactivity, and low-cost features of this new hydrogel system highlight its potential as a stem cell carrier that is capable of bridging the gap between clinic and laboratory.
    Original languageEnglish
    Pages (from-to)12283-12297
    Number of pages15
    JournalACS applied materials & interfaces
    Issue number13
    Publication statusPublished - 3 Apr 2019


    • Tissue engineering
    • Polysaccharide
    • Hydrogel
    • Pectin
    • Gelatin
    • Human mesenchymal stem cells
    • Muscle
    • Neural
    • Bone
    • 22/4 OA procedure


    Dive into the research topics of 'Pectin Methacrylate (PEMA) and Gelatin-Based Hydrogels for Cell Delivery: Converting Waste Materials into Biomaterials'. Together they form a unique fingerprint.

    Cite this