Performance of different three-dimensional scaffolds for in vivo endochondral bone generation

W. Yang, S. K. Both, G. J.V.M. Van Osch, Y. Wang, J. A. Jansen, Fang Yang

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

In the context of skeletal tissue development and repair, endochondral ossification has inspired a new approach to regenerate bone tissue in vivo using a cartilage intermediate as an osteoinductive template. The aim of this study was to investigate the behavior of mesenchymal stem cells (MSCs) in regard to in vitro cartilage formation and in vivo bone regeneration when combined with different threedimensional (3D) scaffold materials, i.e., hydroxyapatite/ tricalcium phosphate (HA/TCP) composite block, polyurethane (PU) foam, poly(lactic-co-glycolic acid)/ poly(ε-caprolactone) electrospun fibers (PLGA/PCL) and collagen I gel. To this end, rat MSCs were seeded on these scaffolds and chondrogenically differentiated in vitro for 4 weeks followed by in vivo subcutaneous implantation for 8 weeks. After in vitro chondrogenic priming, comparable cell amounts and cartilage formation were observed in all types of scaffolds. Nonetheless, the quality and maturity of in vivo ectopic bone formation appeared to be scaffold/ material-dependent. Eight weeks of implantation was not sufficient to ossify the entire PLGA/PCL constructs, albeit a comprehensive remodeling of the cartilage had occurred. For HA/TCP, PU and collagen I scaffolds, more mature bone formation with rich vascularity and marrow stroma development could be observed. These data suggest that chondrogenic priming of MSCs in the presence of different scaffold materials allows the establishment of reliable templates for generating functional endochondral bone tissue in vivo without using osteoinductive growth factors. The morphology and maturity of bone formation can be dictated by the scaffold properties.

Original languageEnglish
Pages (from-to)350-364
Number of pages15
JournalEuropean Cells and Materials
Volume27
DOIs
Publication statusPublished - 10 Jun 2014
Externally publishedYes

Fingerprint

Osteogenesis
Scaffolds
Cartilage
Bone
Mesenchymal Stromal Cells
Bone and Bones
Durapatite
Stem cells
Collagen
Tissue
Hydroxyapatite
Polyurethanes
Bone Regeneration
Phosphates
Intercellular Signaling Peptides and Proteins
Acids
Fibers
Bone Marrow
Gels
Scaffolds (biology)

Keywords

  • Bone regeneration
  • Endochondral bone formation
  • Mesenchymal stem cells
  • Scaffold
  • Subcutaneous implantation

Cite this

Yang, W. ; Both, S. K. ; Van Osch, G. J.V.M. ; Wang, Y. ; Jansen, J. A. ; Yang, Fang. / Performance of different three-dimensional scaffolds for in vivo endochondral bone generation. In: European Cells and Materials. 2014 ; Vol. 27. pp. 350-364.
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Performance of different three-dimensional scaffolds for in vivo endochondral bone generation. / Yang, W.; Both, S. K.; Van Osch, G. J.V.M.; Wang, Y.; Jansen, J. A.; Yang, Fang.

In: European Cells and Materials, Vol. 27, 10.06.2014, p. 350-364.

Research output: Contribution to journalArticleAcademicpeer-review

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