Perfusion Intensity Correlates with Expression Levels of Psoriasis-Related Genes and Proteins

A.G.M. Hendriks, W. Steenbergen, P.L.J.M. Zeeuwen, J. Schalkwijk, E. Hondebrink, M.A.J. Klitsie, R. Schreurs, P.C.M. van de Kerkhof, M.M.B. Seyger

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Background: Previous research revealed heterogeneity in the perfusion intensity within clinically homogenous-Appearing plaques, without differences in erythema. In addition, an increased perfusion was found within the perilesional skin. This raises the question whether the heterogeneity in perfusion found both inside and outside a lesion influences the expression levels of genes and proteins involved in the pathogenesis of psoriasis.

Objectives: To correlate the perfusion intensity to mRNA and protein expression of genes associated with the pathogenesis of psoriasis and to visualize the dynamics of the perfusion intensity over time using laser Doppler perfusion imaging.

Methods: Fourteen patients with plaque psoriasis were included. The superficial microcirculation and clinical local scores (single usability metric, SUM, scores) were analysed in one representative lesion every 2 weeks. After 8 weeks 4 biopsies were taken, one from a highly perfused area (hotspot) and one from a low perfusion area (coldspot) of the lesional skin, one biopsy from the highly perfused perilesional skin and one from the distant uninvolved skin.

Results: Statistically significant differences in mRNA and protein expression, including IL-17 and TBX21/T-Bet, were found between hotspots and coldspots, and between the highly perfused perilesional and the uninvolved skin. Hotspots tend to remain on the same location during 8 weeks of follow-up.

Conclusions: Within homogenous-Appearing psoriatic plaques, there are remarkable differences in mRNA and protein levels, which are correlated with the perfusion intensity and can be detected by using laser Doppler perfusion imaging. In addition, differences in mRNA and protein expression between the highly perfused perilesional skin and the uninvolved skin were found, indicating that several biological changes occur well before clinical changes become manifest.

Original languageEnglish
Pages (from-to)296-306
Number of pages11
JournalSkin Pharmacology and Physiology
Volume28
Issue number6
DOIs
Publication statusPublished - 16 Oct 2015

Fingerprint

Psoriasis
Perfusion
Skin
Proteins
Messenger RNA
Perfusion Imaging
Lasers
Biopsy
Interleukin-17
Erythema
Microcirculation
Gene Expression
Research

Keywords

  • Laser Doppler perfusion imaging
  • Perfusion
  • Plaque heterogeneity
  • Psoriasis
  • Twente Optical Perfusion Camera

Cite this

Hendriks, A. G. M., Steenbergen, W., Zeeuwen, P. L. J. M., Schalkwijk, J., Hondebrink, E., Klitsie, M. A. J., ... Seyger, M. M. B. (2015). Perfusion Intensity Correlates with Expression Levels of Psoriasis-Related Genes and Proteins. Skin Pharmacology and Physiology, 28(6), 296-306. https://doi.org/10.1159/000381628
Hendriks, A.G.M. ; Steenbergen, W. ; Zeeuwen, P.L.J.M. ; Schalkwijk, J. ; Hondebrink, E. ; Klitsie, M.A.J. ; Schreurs, R. ; van de Kerkhof, P.C.M. ; Seyger, M.M.B. / Perfusion Intensity Correlates with Expression Levels of Psoriasis-Related Genes and Proteins. In: Skin Pharmacology and Physiology. 2015 ; Vol. 28, No. 6. pp. 296-306.
@article{f1e083b3cc9f4257be892109223dfcca,
title = "Perfusion Intensity Correlates with Expression Levels of Psoriasis-Related Genes and Proteins",
abstract = "Background: Previous research revealed heterogeneity in the perfusion intensity within clinically homogenous-Appearing plaques, without differences in erythema. In addition, an increased perfusion was found within the perilesional skin. This raises the question whether the heterogeneity in perfusion found both inside and outside a lesion influences the expression levels of genes and proteins involved in the pathogenesis of psoriasis.Objectives: To correlate the perfusion intensity to mRNA and protein expression of genes associated with the pathogenesis of psoriasis and to visualize the dynamics of the perfusion intensity over time using laser Doppler perfusion imaging.Methods: Fourteen patients with plaque psoriasis were included. The superficial microcirculation and clinical local scores (single usability metric, SUM, scores) were analysed in one representative lesion every 2 weeks. After 8 weeks 4 biopsies were taken, one from a highly perfused area (hotspot) and one from a low perfusion area (coldspot) of the lesional skin, one biopsy from the highly perfused perilesional skin and one from the distant uninvolved skin.Results: Statistically significant differences in mRNA and protein expression, including IL-17 and TBX21/T-Bet, were found between hotspots and coldspots, and between the highly perfused perilesional and the uninvolved skin. Hotspots tend to remain on the same location during 8 weeks of follow-up.Conclusions: Within homogenous-Appearing psoriatic plaques, there are remarkable differences in mRNA and protein levels, which are correlated with the perfusion intensity and can be detected by using laser Doppler perfusion imaging. In addition, differences in mRNA and protein expression between the highly perfused perilesional skin and the uninvolved skin were found, indicating that several biological changes occur well before clinical changes become manifest.",
keywords = "Laser Doppler perfusion imaging, Perfusion, Plaque heterogeneity, Psoriasis, Twente Optical Perfusion Camera",
author = "A.G.M. Hendriks and W. Steenbergen and P.L.J.M. Zeeuwen and J. Schalkwijk and E. Hondebrink and M.A.J. Klitsie and R. Schreurs and {van de Kerkhof}, P.C.M. and M.M.B. Seyger",
year = "2015",
month = "10",
day = "16",
doi = "10.1159/000381628",
language = "English",
volume = "28",
pages = "296--306",
journal = "Skin Pharmacology and Physiology",
issn = "1660-5527",
publisher = "Karger",
number = "6",

}

Hendriks, AGM, Steenbergen, W, Zeeuwen, PLJM, Schalkwijk, J, Hondebrink, E, Klitsie, MAJ, Schreurs, R, van de Kerkhof, PCM & Seyger, MMB 2015, 'Perfusion Intensity Correlates with Expression Levels of Psoriasis-Related Genes and Proteins', Skin Pharmacology and Physiology, vol. 28, no. 6, pp. 296-306. https://doi.org/10.1159/000381628

Perfusion Intensity Correlates with Expression Levels of Psoriasis-Related Genes and Proteins. / Hendriks, A.G.M.; Steenbergen, W.; Zeeuwen, P.L.J.M.; Schalkwijk, J.; Hondebrink, E.; Klitsie, M.A.J.; Schreurs, R.; van de Kerkhof, P.C.M.; Seyger, M.M.B.

In: Skin Pharmacology and Physiology, Vol. 28, No. 6, 16.10.2015, p. 296-306.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Perfusion Intensity Correlates with Expression Levels of Psoriasis-Related Genes and Proteins

AU - Hendriks, A.G.M.

AU - Steenbergen, W.

AU - Zeeuwen, P.L.J.M.

AU - Schalkwijk, J.

AU - Hondebrink, E.

AU - Klitsie, M.A.J.

AU - Schreurs, R.

AU - van de Kerkhof, P.C.M.

AU - Seyger, M.M.B.

PY - 2015/10/16

Y1 - 2015/10/16

N2 - Background: Previous research revealed heterogeneity in the perfusion intensity within clinically homogenous-Appearing plaques, without differences in erythema. In addition, an increased perfusion was found within the perilesional skin. This raises the question whether the heterogeneity in perfusion found both inside and outside a lesion influences the expression levels of genes and proteins involved in the pathogenesis of psoriasis.Objectives: To correlate the perfusion intensity to mRNA and protein expression of genes associated with the pathogenesis of psoriasis and to visualize the dynamics of the perfusion intensity over time using laser Doppler perfusion imaging.Methods: Fourteen patients with plaque psoriasis were included. The superficial microcirculation and clinical local scores (single usability metric, SUM, scores) were analysed in one representative lesion every 2 weeks. After 8 weeks 4 biopsies were taken, one from a highly perfused area (hotspot) and one from a low perfusion area (coldspot) of the lesional skin, one biopsy from the highly perfused perilesional skin and one from the distant uninvolved skin.Results: Statistically significant differences in mRNA and protein expression, including IL-17 and TBX21/T-Bet, were found between hotspots and coldspots, and between the highly perfused perilesional and the uninvolved skin. Hotspots tend to remain on the same location during 8 weeks of follow-up.Conclusions: Within homogenous-Appearing psoriatic plaques, there are remarkable differences in mRNA and protein levels, which are correlated with the perfusion intensity and can be detected by using laser Doppler perfusion imaging. In addition, differences in mRNA and protein expression between the highly perfused perilesional skin and the uninvolved skin were found, indicating that several biological changes occur well before clinical changes become manifest.

AB - Background: Previous research revealed heterogeneity in the perfusion intensity within clinically homogenous-Appearing plaques, without differences in erythema. In addition, an increased perfusion was found within the perilesional skin. This raises the question whether the heterogeneity in perfusion found both inside and outside a lesion influences the expression levels of genes and proteins involved in the pathogenesis of psoriasis.Objectives: To correlate the perfusion intensity to mRNA and protein expression of genes associated with the pathogenesis of psoriasis and to visualize the dynamics of the perfusion intensity over time using laser Doppler perfusion imaging.Methods: Fourteen patients with plaque psoriasis were included. The superficial microcirculation and clinical local scores (single usability metric, SUM, scores) were analysed in one representative lesion every 2 weeks. After 8 weeks 4 biopsies were taken, one from a highly perfused area (hotspot) and one from a low perfusion area (coldspot) of the lesional skin, one biopsy from the highly perfused perilesional skin and one from the distant uninvolved skin.Results: Statistically significant differences in mRNA and protein expression, including IL-17 and TBX21/T-Bet, were found between hotspots and coldspots, and between the highly perfused perilesional and the uninvolved skin. Hotspots tend to remain on the same location during 8 weeks of follow-up.Conclusions: Within homogenous-Appearing psoriatic plaques, there are remarkable differences in mRNA and protein levels, which are correlated with the perfusion intensity and can be detected by using laser Doppler perfusion imaging. In addition, differences in mRNA and protein expression between the highly perfused perilesional skin and the uninvolved skin were found, indicating that several biological changes occur well before clinical changes become manifest.

KW - Laser Doppler perfusion imaging

KW - Perfusion

KW - Plaque heterogeneity

KW - Psoriasis

KW - Twente Optical Perfusion Camera

UR - http://www.scopus.com/inward/record.url?scp=84941766417&partnerID=8YFLogxK

U2 - 10.1159/000381628

DO - 10.1159/000381628

M3 - Article

C2 - 26361329

AN - SCOPUS:84941766417

VL - 28

SP - 296

EP - 306

JO - Skin Pharmacology and Physiology

JF - Skin Pharmacology and Physiology

SN - 1660-5527

IS - 6

ER -