Peristaltic on-chip pump for tunable media circulation and whole blood perfusion in PDMS-free organ-on-chip and Organ-Disc systems

Stefan Schneider, Marvin Bubeck, Julia Rogal, Huub J. Weener, Cristhian Rojas, Martin Weiss, Michael Heymann, Andries D. van der Meer, Peter Loskill*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Organ-on-chip (OoC) systems have become a promising tool for personalized medicine and drug development with advantages over conventional animal models and cell assays. However, the utility of OoCs in industrial settings is still limited, as external pumps and tubing for on-chip fluid transport are dependent on error-prone, manual handling. Here, we present an on-chip pump for OoC and Organ-Disc systems, to perfuse media without external pumps or tubing. Peristaltic pumping is implemented through periodic compression of a flexible pump layer. The disc-shaped, microfluidic module contains four independent systems, each lined with endothelial cells cultured under defined, peristaltic perfusion. Both cell viability and functionality were maintained over several days shown by supernatant analysis and immunostaining. Integrated, on-disc perfusion was further used for cytokine-induced cell activation with physiologic cell responses and for whole blood perfusion assays, both demonstrating the versatility of our system for OoC applications.

Original languageEnglish
Pages (from-to)3963-3978
Number of pages16
JournalLab on a chip
Volume21
Issue number20
DOIs
Publication statusPublished - 21 Oct 2021

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