Peritoneal transforming growth factor beta-1 expression during laparoscopic surgery: A clinical trial

Walter J A Brokelman, Lena Holmdahl, Maria Bergström, Peter Falk, Jean H G Klinkonbijl, Michael M P J Reijnen

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

Background: Transforming growth factor-beta 1 (TGF-β1) is a growth factor involved in various biologic processes, including peritoneal wound healing and dissemination of malignancies. Laparoscopic surgery is evolving rapidly, and indications are increasing. The peritoneal TGF-β1 expression during laparoscopic surgery is unknown. Methods: For this study, 50 patients scheduled for laparoscopic cholecystectomy were randomized into five groups, then surgically treated with various pressures, light intensities, and dissection devices. Peritoneal biopsies were taken at the beginning and end of surgery. Tissue concentrations of total and active TGF-β1 were measured using enzyme-linked immunosorbent assay (ELISA) techniques. Results: There was no significant difference in either total or active TGF-β1 concentration between peritoneal biopsies taken at the start of surgery and samples taken at the end of the procedure. Patients who underwent surgery with the ultrasonic scalpel had significant lower levels of both active (p < 0.005) and total (p < 0.01) TGF-β1 at the end of surgery than patients treated with electrocautery. Patients who had surgery with a high light intensity had significantly lower levels of total TGF-β1 levels (p < 0.005) with an unchanged active part than patients who had surgery with low light intensity. Conclusion: The choice of dissection device and the light intensity used in laparoscopic surgery affect peritoneal TGF-β1 concentrations, indicating that peritoneal biology can be affected by laparoscopic surgery. Because TGF-β1 is involved in various biologic processes in the peritoneal cavity, this observation may have important clinical consequences.

Original languageEnglish
Pages (from-to)1537-1541
Number of pages5
JournalSurgical Endoscopy and Other Interventional Techniques
Volume21
Issue number9
DOIs
Publication statusPublished - 1 Sep 2007
Externally publishedYes

Fingerprint

Transforming Growth Factor beta
Laparoscopy
Clinical Trials
Light
Ultrasonic Surgical Procedures
Dissection
Immunosorbent Techniques
Biopsy
Equipment and Supplies
Electrocoagulation
Laparoscopic Cholecystectomy
Peritoneal Cavity
Wound Healing
Intercellular Signaling Peptides and Proteins
Enzyme-Linked Immunosorbent Assay
Pressure
Neoplasms

Keywords

  • Adhesions
  • Fibrinolyse
  • Laparoscopy
  • Peritoneum
  • Transforming growth factor-β1

Cite this

Brokelman, Walter J A ; Holmdahl, Lena ; Bergström, Maria ; Falk, Peter ; Klinkonbijl, Jean H G ; Reijnen, Michael M P J. / Peritoneal transforming growth factor beta-1 expression during laparoscopic surgery : A clinical trial. In: Surgical Endoscopy and Other Interventional Techniques. 2007 ; Vol. 21, No. 9. pp. 1537-1541.
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Peritoneal transforming growth factor beta-1 expression during laparoscopic surgery : A clinical trial. / Brokelman, Walter J A; Holmdahl, Lena; Bergström, Maria; Falk, Peter; Klinkonbijl, Jean H G; Reijnen, Michael M P J.

In: Surgical Endoscopy and Other Interventional Techniques, Vol. 21, No. 9, 01.09.2007, p. 1537-1541.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Peritoneal transforming growth factor beta-1 expression during laparoscopic surgery

T2 - A clinical trial

AU - Brokelman, Walter J A

AU - Holmdahl, Lena

AU - Bergström, Maria

AU - Falk, Peter

AU - Klinkonbijl, Jean H G

AU - Reijnen, Michael M P J

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N2 - Background: Transforming growth factor-beta 1 (TGF-β1) is a growth factor involved in various biologic processes, including peritoneal wound healing and dissemination of malignancies. Laparoscopic surgery is evolving rapidly, and indications are increasing. The peritoneal TGF-β1 expression during laparoscopic surgery is unknown. Methods: For this study, 50 patients scheduled for laparoscopic cholecystectomy were randomized into five groups, then surgically treated with various pressures, light intensities, and dissection devices. Peritoneal biopsies were taken at the beginning and end of surgery. Tissue concentrations of total and active TGF-β1 were measured using enzyme-linked immunosorbent assay (ELISA) techniques. Results: There was no significant difference in either total or active TGF-β1 concentration between peritoneal biopsies taken at the start of surgery and samples taken at the end of the procedure. Patients who underwent surgery with the ultrasonic scalpel had significant lower levels of both active (p < 0.005) and total (p < 0.01) TGF-β1 at the end of surgery than patients treated with electrocautery. Patients who had surgery with a high light intensity had significantly lower levels of total TGF-β1 levels (p < 0.005) with an unchanged active part than patients who had surgery with low light intensity. Conclusion: The choice of dissection device and the light intensity used in laparoscopic surgery affect peritoneal TGF-β1 concentrations, indicating that peritoneal biology can be affected by laparoscopic surgery. Because TGF-β1 is involved in various biologic processes in the peritoneal cavity, this observation may have important clinical consequences.

AB - Background: Transforming growth factor-beta 1 (TGF-β1) is a growth factor involved in various biologic processes, including peritoneal wound healing and dissemination of malignancies. Laparoscopic surgery is evolving rapidly, and indications are increasing. The peritoneal TGF-β1 expression during laparoscopic surgery is unknown. Methods: For this study, 50 patients scheduled for laparoscopic cholecystectomy were randomized into five groups, then surgically treated with various pressures, light intensities, and dissection devices. Peritoneal biopsies were taken at the beginning and end of surgery. Tissue concentrations of total and active TGF-β1 were measured using enzyme-linked immunosorbent assay (ELISA) techniques. Results: There was no significant difference in either total or active TGF-β1 concentration between peritoneal biopsies taken at the start of surgery and samples taken at the end of the procedure. Patients who underwent surgery with the ultrasonic scalpel had significant lower levels of both active (p < 0.005) and total (p < 0.01) TGF-β1 at the end of surgery than patients treated with electrocautery. Patients who had surgery with a high light intensity had significantly lower levels of total TGF-β1 levels (p < 0.005) with an unchanged active part than patients who had surgery with low light intensity. Conclusion: The choice of dissection device and the light intensity used in laparoscopic surgery affect peritoneal TGF-β1 concentrations, indicating that peritoneal biology can be affected by laparoscopic surgery. Because TGF-β1 is involved in various biologic processes in the peritoneal cavity, this observation may have important clinical consequences.

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SN - 0930-2794

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