pH-gradient chromatofocusing of proteins on a chip

Hoon Suk Rho, Alexander Thomas Hanke, Marcel Ottens, J.G.E. Gardeniers

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

We present a novel microfluidic system for the pH-gradient focusing of proteins with the integration of 16 parallel micro-mixers, a micro-column, and a multiplexer. In this work we successfully achieved the creation of 16 non-linear gradients and the generation of a solid-phase micro-column for the realization of anion exchange chromatography on a single chip. With the device we demonstrated the separation of a protein mixture of R-phycoerythrin and FITC-BSA based on pH-gradient chromatofocusing.

Original languageEnglish
Title of host publication19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)
PublisherThe Chemical and Biological Microsystems Society
Pages245-247
Number of pages3
ISBN (Electronic)978-0-9798-0648-3
ISBN (Print)978-1-5108-1787-6
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 - Hwabaek International Convention Center (HICO), Gyeongju, Korea, Republic of
Duration: 25 Oct 201529 Oct 2015
Conference number: 19
http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

Conference

Conference19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015
Abbreviated titleMicroTas 2015
CountryKorea, Republic of
CityGyeongju
Period25/10/1529/10/15
Internet address

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Proteins
Chromatography
Microfluidics
Ion exchange
Negative ions

Keywords

  • Chromatofocusing
  • Micro-column
  • Microfluidics
  • PH gradient

Cite this

Rho, H. S., Hanke, A. T., Ottens, M., & Gardeniers, J. G. E. (2015). pH-gradient chromatofocusing of proteins on a chip. In 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015) (pp. 245-247). The Chemical and Biological Microsystems Society.
Rho, Hoon Suk ; Hanke, Alexander Thomas ; Ottens, Marcel ; Gardeniers, J.G.E. / pH-gradient chromatofocusing of proteins on a chip. 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015). The Chemical and Biological Microsystems Society, 2015. pp. 245-247
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Rho, HS, Hanke, AT, Ottens, M & Gardeniers, JGE 2015, pH-gradient chromatofocusing of proteins on a chip. in 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015). The Chemical and Biological Microsystems Society, pp. 245-247, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015, Gyeongju, Korea, Republic of, 25/10/15.

pH-gradient chromatofocusing of proteins on a chip. / Rho, Hoon Suk; Hanke, Alexander Thomas; Ottens, Marcel; Gardeniers, J.G.E.

19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015). The Chemical and Biological Microsystems Society, 2015. p. 245-247.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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Rho HS, Hanke AT, Ottens M, Gardeniers JGE. pH-gradient chromatofocusing of proteins on a chip. In 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015). The Chemical and Biological Microsystems Society. 2015. p. 245-247