pH-gradient chromatofocusing of proteins on a chip

Hoon Suk Rho; Alexander Thomas Hanke; Marcel Ottens; J.G.E. Gardeniers

pH-gradient chromatofocusing of proteins on a chip

Hoon Suk Rho
, Alexander Thomas Hanke
, Marcel Ottens
, J.G.E. Gardeniers^*

^*Corresponding author for this work

Mesoscale Chemical Systems

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

We present a novel microfluidic system for the pH-gradient focusing of proteins with the integration of 16 parallel micro-mixers, a micro-column, and a multiplexer. In this work we successfully achieved the creation of 16 non-linear gradients and the generation of a solid-phase micro-column for the realization of anion exchange chromatography on a single chip. With the device we demonstrated the separation of a protein mixture of R-phycoerythrin and FITC-BSA based on pH-gradient chromatofocusing.

Original language	English
Title of host publication	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)
Publisher	The Chemical and Biological Microsystems Society
Pages	245-247
Number of pages	3
ISBN (Electronic)	978-0-9798-0648-3
ISBN (Print)	978-1-5108-1787-6
Publication status	Published - 2015
Event	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 - Hwabaek International Convention Center (HICO), Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 Conference number: 19 http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

Conference

Conference	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015
Abbreviated title	MicroTas 2015
Country/Territory	Korea, Republic of
City	Gyeongju
Period	25/10/15 → 29/10/15
Internet address	http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf

Keywords

Chromatofocusing
Micro-column
Microfluidics
PH gradient

Cite this

@inproceedings{6bd985281209483a8e9dd05eedded0d6,

title = "pH-gradient chromatofocusing of proteins on a chip",

abstract = "We present a novel microfluidic system for the pH-gradient focusing of proteins with the integration of 16 parallel micro-mixers, a micro-column, and a multiplexer. In this work we successfully achieved the creation of 16 non-linear gradients and the generation of a solid-phase micro-column for the realization of anion exchange chromatography on a single chip. With the device we demonstrated the separation of a protein mixture of R-phycoerythrin and FITC-BSA based on pH-gradient chromatofocusing.",

keywords = "Chromatofocusing, Micro-column, Microfluidics, PH gradient",

author = "Rho, \{Hoon Suk\} and Hanke, \{Alexander Thomas\} and Marcel Ottens and J.G.E. Gardeniers",

year = "2015",

language = "English",

isbn = "978-1-5108-1787-6",

pages = "245--247",

booktitle = "19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)",

publisher = "The Chemical and Biological Microsystems Society",

note = "19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015, MicroTas 2015 ; Conference date: 25-10-2015 Through 29-10-2015",

url = "http://www.rsc.org/images/LOC/2015/PDFs/Copyrite.pdf",

}

Rho, HS, Hanke, AT, Ottens, M & Gardeniers, JGE 2015, pH-gradient chromatofocusing of proteins on a chip. in 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015). The Chemical and Biological Microsystems Society, pp. 245-247, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015, Gyeongju, Korea, Republic of, 25/10/15.

pH-gradient chromatofocusing of proteins on a chip. / Rho, Hoon Suk; Hanke, Alexander Thomas; Ottens, Marcel et al.
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015). The Chemical and Biological Microsystems Society, 2015. p. 245-247.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - pH-gradient chromatofocusing of proteins on a chip

AU - Rho, Hoon Suk

AU - Hanke, Alexander Thomas

AU - Ottens, Marcel

AU - Gardeniers, J.G.E.

N1 - Conference code: 19

PY - 2015

Y1 - 2015

N2 - We present a novel microfluidic system for the pH-gradient focusing of proteins with the integration of 16 parallel micro-mixers, a micro-column, and a multiplexer. In this work we successfully achieved the creation of 16 non-linear gradients and the generation of a solid-phase micro-column for the realization of anion exchange chromatography on a single chip. With the device we demonstrated the separation of a protein mixture of R-phycoerythrin and FITC-BSA based on pH-gradient chromatofocusing.

AB - We present a novel microfluidic system for the pH-gradient focusing of proteins with the integration of 16 parallel micro-mixers, a micro-column, and a multiplexer. In this work we successfully achieved the creation of 16 non-linear gradients and the generation of a solid-phase micro-column for the realization of anion exchange chromatography on a single chip. With the device we demonstrated the separation of a protein mixture of R-phycoerythrin and FITC-BSA based on pH-gradient chromatofocusing.

KW - Chromatofocusing

KW - Micro-column

KW - Microfluidics

KW - PH gradient

UR - https://www.scopus.com/pages/publications/84983371690

M3 - Conference contribution

AN - SCOPUS:84983371690

SN - 978-1-5108-1787-6

SP - 245

EP - 247

BT - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)

PB - The Chemical and Biological Microsystems Society

T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015

Y2 - 25 October 2015 through 29 October 2015

ER -

pH-gradient chromatofocusing of proteins on a chip

Abstract

Conference

Keywords

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