Phase 1 and 2 drug metabolites generated using a miniaturized electrochemical cell with an attached esi needle

Floris Teunis Gerardus van den Brink, Lars Büter, Mathieu Odijk, Wouter Olthuis, U. Karst, Albert van den Berg

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    Abstract

    In this work we show phase 1 and 2 drug metabolism using a miniaturized electrochemical cell followed by on-line metabolite detection using electrospray ionization – mass spectrometry (ESI-MS). The most common form of in vivo drug metabolism is enzyme-catalyzed oxidation [1]. Therefore, a method to rapidly mimic these reactions and subsequently analyze the products is interesting for drug screening applications. With the versatile experimental setup presented, phase 1 metabolism of clozapine is carried out with a very short transfer time to detection, as well as phase 1 and 2 metabolism of paracetamol which is first oxidized and subsequently conjugated to glutathione
    Original languageUndefined
    Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS
    EditorsR. Zengerle
    Place of PublicationFreiburg
    PublisherThe Printing House
    Pages11-13
    Number of pages3
    ISBN (Print)978-0-9798064-6-9
    Publication statusPublished - 27 Oct 2013
    Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013 - Freiburg, Germany
    Duration: 27 Oct 201331 Oct 2013
    Conference number: 17

    Publication series

    NameMicroTAS
    PublisherThe Printing House
    NumberMicroTAS 2013
    ISSN (Print)1556-5904

    Conference

    Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013
    Abbreviated titleMicroTAS
    CountryGermany
    CityFreiburg
    Period27/10/1331/10/13

    Keywords

    • EWI-24045
    • METIS-300191
    • IR-88072

    Cite this

    van den Brink, F. T. G., Büter, L., Odijk, M., Olthuis, W., Karst, U., & van den Berg, A. (2013). Phase 1 and 2 drug metabolites generated using a miniaturized electrochemical cell with an attached esi needle. In R. Zengerle (Ed.), 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS (pp. 11-13). (MicroTAS; No. MicroTAS 2013). Freiburg: The Printing House.