Phase 1 and 2 drug metabolites generated using a miniaturized electrochemical cell with an attached esi needle

Floris Teunis Gerardus van den Brink, Lars Büter, Mathieu Odijk, Wouter Olthuis, U. Karst, Albert van den Berg

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Abstract

In this work we show phase 1 and 2 drug metabolism using a miniaturized electrochemical cell followed by on-line metabolite detection using electrospray ionization – mass spectrometry (ESI-MS). The most common form of in vivo drug metabolism is enzyme-catalyzed oxidation [1]. Therefore, a method to rapidly mimic these reactions and subsequently analyze the products is interesting for drug screening applications. With the versatile experimental setup presented, phase 1 metabolism of clozapine is carried out with a very short transfer time to detection, as well as phase 1 and 2 metabolism of paracetamol which is first oxidized and subsequently conjugated to glutathione
Original languageUndefined
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS
EditorsR. Zengerle
Place of PublicationFreiburg
PublisherThe Printing House
Pages11-13
Number of pages3
ISBN (Print)978-0-9798064-6-9
Publication statusPublished - 27 Oct 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013 - Freiburg, Germany
Duration: 27 Oct 201331 Oct 2013
Conference number: 17

Publication series

NameMicroTAS
PublisherThe Printing House
NumberMicroTAS 2013
ISSN (Print)1556-5904

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013
Abbreviated titleMicroTAS
CountryGermany
CityFreiburg
Period27/10/1331/10/13

Keywords

  • EWI-24045
  • METIS-300191
  • IR-88072

Cite this

van den Brink, F. T. G., Büter, L., Odijk, M., Olthuis, W., Karst, U., & van den Berg, A. (2013). Phase 1 and 2 drug metabolites generated using a miniaturized electrochemical cell with an attached esi needle. In R. Zengerle (Ed.), 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS (pp. 11-13). (MicroTAS; No. MicroTAS 2013). Freiburg: The Printing House.
van den Brink, Floris Teunis Gerardus ; Büter, Lars ; Odijk, Mathieu ; Olthuis, Wouter ; Karst, U. ; van den Berg, Albert. / Phase 1 and 2 drug metabolites generated using a miniaturized electrochemical cell with an attached esi needle. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS. editor / R. Zengerle. Freiburg : The Printing House, 2013. pp. 11-13 (MicroTAS; MicroTAS 2013).
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abstract = "In this work we show phase 1 and 2 drug metabolism using a miniaturized electrochemical cell followed by on-line metabolite detection using electrospray ionization – mass spectrometry (ESI-MS). The most common form of in vivo drug metabolism is enzyme-catalyzed oxidation [1]. Therefore, a method to rapidly mimic these reactions and subsequently analyze the products is interesting for drug screening applications. With the versatile experimental setup presented, phase 1 metabolism of clozapine is carried out with a very short transfer time to detection, as well as phase 1 and 2 metabolism of paracetamol which is first oxidized and subsequently conjugated to glutathione",
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isbn = "978-0-9798064-6-9",
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van den Brink, FTG, Büter, L, Odijk, M, Olthuis, W, Karst, U & van den Berg, A 2013, Phase 1 and 2 drug metabolites generated using a miniaturized electrochemical cell with an attached esi needle. in R Zengerle (ed.), 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS. MicroTAS, no. MicroTAS 2013, The Printing House, Freiburg, pp. 11-13, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013, Freiburg, Germany, 27/10/13.

Phase 1 and 2 drug metabolites generated using a miniaturized electrochemical cell with an attached esi needle. / van den Brink, Floris Teunis Gerardus; Büter, Lars; Odijk, Mathieu; Olthuis, Wouter; Karst, U.; van den Berg, Albert.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS. ed. / R. Zengerle. Freiburg : The Printing House, 2013. p. 11-13 (MicroTAS; No. MicroTAS 2013).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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T1 - Phase 1 and 2 drug metabolites generated using a miniaturized electrochemical cell with an attached esi needle

AU - van den Brink, Floris Teunis Gerardus

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AU - Olthuis, Wouter

AU - Karst, U.

AU - van den Berg, Albert

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Y1 - 2013/10/27

N2 - In this work we show phase 1 and 2 drug metabolism using a miniaturized electrochemical cell followed by on-line metabolite detection using electrospray ionization – mass spectrometry (ESI-MS). The most common form of in vivo drug metabolism is enzyme-catalyzed oxidation [1]. Therefore, a method to rapidly mimic these reactions and subsequently analyze the products is interesting for drug screening applications. With the versatile experimental setup presented, phase 1 metabolism of clozapine is carried out with a very short transfer time to detection, as well as phase 1 and 2 metabolism of paracetamol which is first oxidized and subsequently conjugated to glutathione

AB - In this work we show phase 1 and 2 drug metabolism using a miniaturized electrochemical cell followed by on-line metabolite detection using electrospray ionization – mass spectrometry (ESI-MS). The most common form of in vivo drug metabolism is enzyme-catalyzed oxidation [1]. Therefore, a method to rapidly mimic these reactions and subsequently analyze the products is interesting for drug screening applications. With the versatile experimental setup presented, phase 1 metabolism of clozapine is carried out with a very short transfer time to detection, as well as phase 1 and 2 metabolism of paracetamol which is first oxidized and subsequently conjugated to glutathione

KW - EWI-24045

KW - METIS-300191

KW - IR-88072

M3 - Conference contribution

SN - 978-0-9798064-6-9

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van den Brink FTG, Büter L, Odijk M, Olthuis W, Karst U, van den Berg A. Phase 1 and 2 drug metabolites generated using a miniaturized electrochemical cell with an attached esi needle. In Zengerle R, editor, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS. Freiburg: The Printing House. 2013. p. 11-13. (MicroTAS; MicroTAS 2013).