Photoacoustic Nodal Metastases detection in a rat model using a clinical superparamagnetic iron oxide nanoparticle dispersion

Research output: Contribution to conferencePosterOther research output

Abstract

Background: Detection of tumor metastases in the lymphatic system is essential for accurate staging of different malignancies, however fast and accurate pre- or intra-operative evaluation of the nodal status remains difficult to perform with common available medical imaging techniques. In recent years, numerous studies have confirmed the additional value of superparamagnetic iron oxide dispersions (SPIOs) for nodal staging purposes, prompting the clearance of different SPIO dispersions for clinical practice. Here we evaluate whether a combination of photoacoustic (PA) imaging and a clinically approved SPIO dispersion, could have the potential for sensitive assessment of the nodal status both pre- and intra-operatively.

Materials and methods: Prostate adenocarcinoma cells (MAT-lylu) were inoculated in the footpad of Copenhagen rats. For lymph node imaging animals received a subcutaneous injection of a SPIO dispersion (Endorem®, Guerbet, France) either 5 (n=4) or 8 (n=4) days after tumor inoculation. The resected nodes were photoacoustically imaged at 720 nm using 20 mJ/cm2, 24 hours later, and imaging results were compared to 14 Tesla MR imaging and histology. Control animals were imaged without SPIO injection in the involved leg or received SPIO injections on the contra lateral site harboring no tumor inoculation and hence normal lymph nodes.

Results: In the tumor groups clear irregularities, as small as 1 mm, were observed in the PA contrast pattern of the nodes. These irregularities could be correlated to the absence of contrast in the MR images and could directly be linked to metastatic deposits seen on the histological slides. The PA and MR images of the control animals did not show these irregularities.

Conclusions: We conclude that the combination of photoacoustic imaging with a clinically approved iron oxide nanoparticle dispersion is able to detect lymph node metastases as small as 1 mm. This approach opens up new possibilities for pre- or intra-operative nodal staging.
Original languageEnglish
Pages-
DOIs
Publication statusPublished - 19 Sep 2012
Event32nd Congress of the European Society of Surgical Oncology 2012: Individualising Cancer Surgery - Valencia, Spain
Duration: 19 Sep 201221 Sep 2012
Conference number: 32
http://www.ecco-org.eu/Events/Past-conferences/ESSO-32

Conference

Conference32nd Congress of the European Society of Surgical Oncology 2012
CountrySpain
CityValencia
Period19/09/1221/09/12
Internet address

Fingerprint

Nanoparticles
Neoplasm Metastasis
Lymph Nodes
Neoplasms
Lymphatic System
Injections
Diagnostic Imaging
Subcutaneous Injections
ferric oxide
France
Prostate
Leg
Histology
Adenocarcinoma

Keywords

  • METIS-289273

Cite this

Grootendorst, D., Fratila, R. M., Visscher, M., ten Haken, B., van Wezel, R. J. A., Steenbergen, W., ... Ruers, T. J. M. (2012). Photoacoustic Nodal Metastases detection in a rat model using a clinical superparamagnetic iron oxide nanoparticle dispersion. -. Poster session presented at 32nd Congress of the European Society of Surgical Oncology 2012, Valencia, Spain. https://doi.org/10.1016/j.ejso.2012.06.349
Grootendorst, Diederik ; Fratila, R.M. ; Visscher, M. ; ten Haken, Bernard ; van Wezel, Richard Jack Anton ; Steenbergen, Wiendelt ; Manohar, Srirang ; Ruers, Theo J.M. / Photoacoustic Nodal Metastases detection in a rat model using a clinical superparamagnetic iron oxide nanoparticle dispersion. Poster session presented at 32nd Congress of the European Society of Surgical Oncology 2012, Valencia, Spain.
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title = "Photoacoustic Nodal Metastases detection in a rat model using a clinical superparamagnetic iron oxide nanoparticle dispersion",
abstract = "Background: Detection of tumor metastases in the lymphatic system is essential for accurate staging of different malignancies, however fast and accurate pre- or intra-operative evaluation of the nodal status remains difficult to perform with common available medical imaging techniques. In recent years, numerous studies have confirmed the additional value of superparamagnetic iron oxide dispersions (SPIOs) for nodal staging purposes, prompting the clearance of different SPIO dispersions for clinical practice. Here we evaluate whether a combination of photoacoustic (PA) imaging and a clinically approved SPIO dispersion, could have the potential for sensitive assessment of the nodal status both pre- and intra-operatively.Materials and methods: Prostate adenocarcinoma cells (MAT-lylu) were inoculated in the footpad of Copenhagen rats. For lymph node imaging animals received a subcutaneous injection of a SPIO dispersion (Endorem{\circledR}, Guerbet, France) either 5 (n=4) or 8 (n=4) days after tumor inoculation. The resected nodes were photoacoustically imaged at 720 nm using 20 mJ/cm2, 24 hours later, and imaging results were compared to 14 Tesla MR imaging and histology. Control animals were imaged without SPIO injection in the involved leg or received SPIO injections on the contra lateral site harboring no tumor inoculation and hence normal lymph nodes.Results: In the tumor groups clear irregularities, as small as 1 mm, were observed in the PA contrast pattern of the nodes. These irregularities could be correlated to the absence of contrast in the MR images and could directly be linked to metastatic deposits seen on the histological slides. The PA and MR images of the control animals did not show these irregularities.Conclusions: We conclude that the combination of photoacoustic imaging with a clinically approved iron oxide nanoparticle dispersion is able to detect lymph node metastases as small as 1 mm. This approach opens up new possibilities for pre- or intra-operative nodal staging.",
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author = "Diederik Grootendorst and R.M. Fratila and M. Visscher and {ten Haken}, Bernard and {van Wezel}, {Richard Jack Anton} and Wiendelt Steenbergen and Srirang Manohar and Ruers, {Theo J.M.}",
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Grootendorst, D, Fratila, RM, Visscher, M, ten Haken, B, van Wezel, RJA, Steenbergen, W, Manohar, S & Ruers, TJM 2012, 'Photoacoustic Nodal Metastases detection in a rat model using a clinical superparamagnetic iron oxide nanoparticle dispersion' 32nd Congress of the European Society of Surgical Oncology 2012, Valencia, Spain, 19/09/12 - 21/09/12, pp. -. https://doi.org/10.1016/j.ejso.2012.06.349

Photoacoustic Nodal Metastases detection in a rat model using a clinical superparamagnetic iron oxide nanoparticle dispersion. / Grootendorst, Diederik; Fratila, R.M.; Visscher, M.; ten Haken, Bernard; van Wezel, Richard Jack Anton; Steenbergen, Wiendelt; Manohar, Srirang; Ruers, Theo J.M.

2012. - Poster session presented at 32nd Congress of the European Society of Surgical Oncology 2012, Valencia, Spain.

Research output: Contribution to conferencePosterOther research output

TY - CONF

T1 - Photoacoustic Nodal Metastases detection in a rat model using a clinical superparamagnetic iron oxide nanoparticle dispersion

AU - Grootendorst, Diederik

AU - Fratila, R.M.

AU - Visscher, M.

AU - ten Haken, Bernard

AU - van Wezel, Richard Jack Anton

AU - Steenbergen, Wiendelt

AU - Manohar, Srirang

AU - Ruers, Theo J.M.

PY - 2012/9/19

Y1 - 2012/9/19

N2 - Background: Detection of tumor metastases in the lymphatic system is essential for accurate staging of different malignancies, however fast and accurate pre- or intra-operative evaluation of the nodal status remains difficult to perform with common available medical imaging techniques. In recent years, numerous studies have confirmed the additional value of superparamagnetic iron oxide dispersions (SPIOs) for nodal staging purposes, prompting the clearance of different SPIO dispersions for clinical practice. Here we evaluate whether a combination of photoacoustic (PA) imaging and a clinically approved SPIO dispersion, could have the potential for sensitive assessment of the nodal status both pre- and intra-operatively.Materials and methods: Prostate adenocarcinoma cells (MAT-lylu) were inoculated in the footpad of Copenhagen rats. For lymph node imaging animals received a subcutaneous injection of a SPIO dispersion (Endorem®, Guerbet, France) either 5 (n=4) or 8 (n=4) days after tumor inoculation. The resected nodes were photoacoustically imaged at 720 nm using 20 mJ/cm2, 24 hours later, and imaging results were compared to 14 Tesla MR imaging and histology. Control animals were imaged without SPIO injection in the involved leg or received SPIO injections on the contra lateral site harboring no tumor inoculation and hence normal lymph nodes.Results: In the tumor groups clear irregularities, as small as 1 mm, were observed in the PA contrast pattern of the nodes. These irregularities could be correlated to the absence of contrast in the MR images and could directly be linked to metastatic deposits seen on the histological slides. The PA and MR images of the control animals did not show these irregularities.Conclusions: We conclude that the combination of photoacoustic imaging with a clinically approved iron oxide nanoparticle dispersion is able to detect lymph node metastases as small as 1 mm. This approach opens up new possibilities for pre- or intra-operative nodal staging.

AB - Background: Detection of tumor metastases in the lymphatic system is essential for accurate staging of different malignancies, however fast and accurate pre- or intra-operative evaluation of the nodal status remains difficult to perform with common available medical imaging techniques. In recent years, numerous studies have confirmed the additional value of superparamagnetic iron oxide dispersions (SPIOs) for nodal staging purposes, prompting the clearance of different SPIO dispersions for clinical practice. Here we evaluate whether a combination of photoacoustic (PA) imaging and a clinically approved SPIO dispersion, could have the potential for sensitive assessment of the nodal status both pre- and intra-operatively.Materials and methods: Prostate adenocarcinoma cells (MAT-lylu) were inoculated in the footpad of Copenhagen rats. For lymph node imaging animals received a subcutaneous injection of a SPIO dispersion (Endorem®, Guerbet, France) either 5 (n=4) or 8 (n=4) days after tumor inoculation. The resected nodes were photoacoustically imaged at 720 nm using 20 mJ/cm2, 24 hours later, and imaging results were compared to 14 Tesla MR imaging and histology. Control animals were imaged without SPIO injection in the involved leg or received SPIO injections on the contra lateral site harboring no tumor inoculation and hence normal lymph nodes.Results: In the tumor groups clear irregularities, as small as 1 mm, were observed in the PA contrast pattern of the nodes. These irregularities could be correlated to the absence of contrast in the MR images and could directly be linked to metastatic deposits seen on the histological slides. The PA and MR images of the control animals did not show these irregularities.Conclusions: We conclude that the combination of photoacoustic imaging with a clinically approved iron oxide nanoparticle dispersion is able to detect lymph node metastases as small as 1 mm. This approach opens up new possibilities for pre- or intra-operative nodal staging.

KW - METIS-289273

U2 - 10.1016/j.ejso.2012.06.349

DO - 10.1016/j.ejso.2012.06.349

M3 - Poster

SP - -

ER -

Grootendorst D, Fratila RM, Visscher M, ten Haken B, van Wezel RJA, Steenbergen W et al. Photoacoustic Nodal Metastases detection in a rat model using a clinical superparamagnetic iron oxide nanoparticle dispersion. 2012. Poster session presented at 32nd Congress of the European Society of Surgical Oncology 2012, Valencia, Spain. https://doi.org/10.1016/j.ejso.2012.06.349