photoacoustic staging of nodal metastases using spios: comparison between in vivi, in toto and ex vivo imaging in a rat model

Diederik Grootendorst, R.M. Fratila, Joost Jacob Pouw, Bernard ten Haken, Richard Jack Anton van Wezel, Sven Rottenberg, Wiendelt Steenbergen, Srirang Manohar, Theo J.M. Ruers

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background and objectives: To determine prognosis and treatment, accurate nodal staging is essential in many tumor types. After injection of clinical grade superparamagnetic iron oxide (SPIO) nanoparticles, it has been shown that metastatic lymph nodes can be distinguished from benign specimens using MR imaging. However, MR does not benefit per-operative nodal staging which requires a non-ionizing, small volume, high resolution, fast imaging technique. In vivo non-invasive photoacoustic (PA) imaging of lymph nodes might facilitate nodal staging during surgery, thereby benefiting both surgeon and patient. Materials and methods: In order to investigate the feasibility of an in vivo nodal staging approach using photoacoustics, six Mat-lylu inoculated Copenhagen rats were photo-acoustically imaged after injection of a new Class IIa medical device SPIO magnetic tracer (Sienna+). Lymph nodes were imaged in vivo, in toto (after euthanization) and ex vivo using multiple wavelength illumination. Results were compared with MRI, immunohistochemistry and photographs of the sectioned nodes. Results: These experiments demonstrate that in an ex vivo setting, the PA contrast of Sienna+ is able to facilitate a distinction between metastatic and benign nodes. A non-invasive distinction between both groups is partially impeded by the low amount of PA contrast generated by the SPIO particles compared to that of endogenous absorbers such as hemoglobins. Conclusions: This comparison between in vivo, in toto and ex vivo PA imaging of lymph nodes after SPIO injection demonstrates that the clinical potential of combined PA/SPIO staging should initially be exploited in an ex vivo setting. Improved distinction between chromophores by for example multi-spectral unmixing might in the near future enable non-invasive assessment of nodal involvement.
Original languageUndefined
Pages (from-to)71-87
Number of pages17
JournalBiomedical spectroscopy and imaging
Volume5
Issue number1
DOIs
Publication statusPublished - 14 Jan 2016

Keywords

  • EWI-27822
  • SPIONS
  • sentinel lymph nodes (SLN)
  • in vivo and in toto
  • non-invasive
  • optoacoustic
  • photoacoustic
  • METIS-314219
  • IR-100102

Cite this

@article{6df884238a174cc5b9091debdd2b6d81,
title = "photoacoustic staging of nodal metastases using spios: comparison between in vivi, in toto and ex vivo imaging in a rat model",
abstract = "Background and objectives: To determine prognosis and treatment, accurate nodal staging is essential in many tumor types. After injection of clinical grade superparamagnetic iron oxide (SPIO) nanoparticles, it has been shown that metastatic lymph nodes can be distinguished from benign specimens using MR imaging. However, MR does not benefit per-operative nodal staging which requires a non-ionizing, small volume, high resolution, fast imaging technique. In vivo non-invasive photoacoustic (PA) imaging of lymph nodes might facilitate nodal staging during surgery, thereby benefiting both surgeon and patient. Materials and methods: In order to investigate the feasibility of an in vivo nodal staging approach using photoacoustics, six Mat-lylu inoculated Copenhagen rats were photo-acoustically imaged after injection of a new Class IIa medical device SPIO magnetic tracer (Sienna+). Lymph nodes were imaged in vivo, in toto (after euthanization) and ex vivo using multiple wavelength illumination. Results were compared with MRI, immunohistochemistry and photographs of the sectioned nodes. Results: These experiments demonstrate that in an ex vivo setting, the PA contrast of Sienna+ is able to facilitate a distinction between metastatic and benign nodes. A non-invasive distinction between both groups is partially impeded by the low amount of PA contrast generated by the SPIO particles compared to that of endogenous absorbers such as hemoglobins. Conclusions: This comparison between in vivo, in toto and ex vivo PA imaging of lymph nodes after SPIO injection demonstrates that the clinical potential of combined PA/SPIO staging should initially be exploited in an ex vivo setting. Improved distinction between chromophores by for example multi-spectral unmixing might in the near future enable non-invasive assessment of nodal involvement.",
keywords = "EWI-27822, SPIONS, sentinel lymph nodes (SLN), in vivo and in toto, non-invasive, optoacoustic, photoacoustic, METIS-314219, IR-100102",
author = "Diederik Grootendorst and R.M. Fratila and Pouw, {Joost Jacob} and {ten Haken}, Bernard and {van Wezel}, {Richard Jack Anton} and Sven Rottenberg and Wiendelt Steenbergen and Srirang Manohar and Ruers, {Theo J.M.}",
year = "2016",
month = "1",
day = "14",
doi = "10.3233/BSI-150127",
language = "Undefined",
volume = "5",
pages = "71--87",
journal = "Biomedical spectroscopy and imaging",
issn = "2212-8794",
publisher = "IOS Press",
number = "1",

}

photoacoustic staging of nodal metastases using spios: comparison between in vivi, in toto and ex vivo imaging in a rat model. / Grootendorst, Diederik; Fratila, R.M.; Pouw, Joost Jacob; ten Haken, Bernard; van Wezel, Richard Jack Anton; Rottenberg, Sven; Steenbergen, Wiendelt; Manohar, Srirang; Ruers, Theo J.M.

In: Biomedical spectroscopy and imaging, Vol. 5, No. 1, 14.01.2016, p. 71-87.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - photoacoustic staging of nodal metastases using spios: comparison between in vivi, in toto and ex vivo imaging in a rat model

AU - Grootendorst, Diederik

AU - Fratila, R.M.

AU - Pouw, Joost Jacob

AU - ten Haken, Bernard

AU - van Wezel, Richard Jack Anton

AU - Rottenberg, Sven

AU - Steenbergen, Wiendelt

AU - Manohar, Srirang

AU - Ruers, Theo J.M.

PY - 2016/1/14

Y1 - 2016/1/14

N2 - Background and objectives: To determine prognosis and treatment, accurate nodal staging is essential in many tumor types. After injection of clinical grade superparamagnetic iron oxide (SPIO) nanoparticles, it has been shown that metastatic lymph nodes can be distinguished from benign specimens using MR imaging. However, MR does not benefit per-operative nodal staging which requires a non-ionizing, small volume, high resolution, fast imaging technique. In vivo non-invasive photoacoustic (PA) imaging of lymph nodes might facilitate nodal staging during surgery, thereby benefiting both surgeon and patient. Materials and methods: In order to investigate the feasibility of an in vivo nodal staging approach using photoacoustics, six Mat-lylu inoculated Copenhagen rats were photo-acoustically imaged after injection of a new Class IIa medical device SPIO magnetic tracer (Sienna+). Lymph nodes were imaged in vivo, in toto (after euthanization) and ex vivo using multiple wavelength illumination. Results were compared with MRI, immunohistochemistry and photographs of the sectioned nodes. Results: These experiments demonstrate that in an ex vivo setting, the PA contrast of Sienna+ is able to facilitate a distinction between metastatic and benign nodes. A non-invasive distinction between both groups is partially impeded by the low amount of PA contrast generated by the SPIO particles compared to that of endogenous absorbers such as hemoglobins. Conclusions: This comparison between in vivo, in toto and ex vivo PA imaging of lymph nodes after SPIO injection demonstrates that the clinical potential of combined PA/SPIO staging should initially be exploited in an ex vivo setting. Improved distinction between chromophores by for example multi-spectral unmixing might in the near future enable non-invasive assessment of nodal involvement.

AB - Background and objectives: To determine prognosis and treatment, accurate nodal staging is essential in many tumor types. After injection of clinical grade superparamagnetic iron oxide (SPIO) nanoparticles, it has been shown that metastatic lymph nodes can be distinguished from benign specimens using MR imaging. However, MR does not benefit per-operative nodal staging which requires a non-ionizing, small volume, high resolution, fast imaging technique. In vivo non-invasive photoacoustic (PA) imaging of lymph nodes might facilitate nodal staging during surgery, thereby benefiting both surgeon and patient. Materials and methods: In order to investigate the feasibility of an in vivo nodal staging approach using photoacoustics, six Mat-lylu inoculated Copenhagen rats were photo-acoustically imaged after injection of a new Class IIa medical device SPIO magnetic tracer (Sienna+). Lymph nodes were imaged in vivo, in toto (after euthanization) and ex vivo using multiple wavelength illumination. Results were compared with MRI, immunohistochemistry and photographs of the sectioned nodes. Results: These experiments demonstrate that in an ex vivo setting, the PA contrast of Sienna+ is able to facilitate a distinction between metastatic and benign nodes. A non-invasive distinction between both groups is partially impeded by the low amount of PA contrast generated by the SPIO particles compared to that of endogenous absorbers such as hemoglobins. Conclusions: This comparison between in vivo, in toto and ex vivo PA imaging of lymph nodes after SPIO injection demonstrates that the clinical potential of combined PA/SPIO staging should initially be exploited in an ex vivo setting. Improved distinction between chromophores by for example multi-spectral unmixing might in the near future enable non-invasive assessment of nodal involvement.

KW - EWI-27822

KW - SPIONS

KW - sentinel lymph nodes (SLN)

KW - in vivo and in toto

KW - non-invasive

KW - optoacoustic

KW - photoacoustic

KW - METIS-314219

KW - IR-100102

U2 - 10.3233/BSI-150127

DO - 10.3233/BSI-150127

M3 - Article

VL - 5

SP - 71

EP - 87

JO - Biomedical spectroscopy and imaging

JF - Biomedical spectroscopy and imaging

SN - 2212-8794

IS - 1

ER -