Photon-Counting Device Compatible with Conventional Flow Cytometric Data Acquisition Electronics

Alexandra Agronskaia, Alex Florians, Kees O. van der Werf, Juleon M. Schins, Bart G. de Grooth, Jan Greve

    Research output: Contribution to journalArticleAcademicpeer-review

    10 Citations (Scopus)

    Abstract

    We present an electronic scheme that enables us to use a photon-counting device (photomultiplier or avalanche photodetector) for measuring extremely weak signals in a flow cytometer. It can be used as a sole detector, or in combination with other (conventional) detectors using the data acquisition hardware of a conventional flow cytometer. The essential principle is that photon-counting pulses are converted to an analogue signal that is continuously proportional to the number of detected photons during the last integration time. The integration time should be approximately equal to the time an object is illuminated in the flow chamber. In this way, the photon burst due to real events is measured correctly and discriminated from the background pulses (fluorescence and Raman). The use of this scheme for the measurement of single DNA molecules is illustrated.
    Original languageEnglish
    Pages (from-to)255-259
    Number of pages5
    JournalCytometry
    Volume32
    Issue number3
    DOIs
    Publication statusPublished - 1998

    Keywords

    • Single molecule measurements
    • Photon-counting
    • Flow cytometry

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