Photonic effects on the fluorescence lifetimes of dyes in thin PVA layers

Jord C. Prangsma, Robert Molenaar, Vinod Subramaniam, Christian Blum

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

In this paper we investigate the expected change in fluorescent decay rate when a fluorophore in aqueous solution is moved to a thin spin-coated layer of poly(vinyl alcohol). We take into account the local field effect due to the change in the refractive index of the medium around the fluorophore and the photonic effect due to the layers. The obtained results are compared with experimental results for the organic dye Atto565 and the fluorescent protein mCherry. We find that the effects for the organic dye can be well described with the model, for the fluorescent protein (FP) the model is less accurate. We discuss the possible explanations for this.

Original languageEnglish
Title of host publicationNanoimaging and Nanospectroscopy III
PublisherSPIE
Volume9554
ISBN (Electronic)9781628417203
DOIs
Publication statusPublished - 26 Aug 2015

Keywords

  • fluorescent protein
  • local density of states
  • local field effect
  • PVA

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