Photonic effects on the fluorescence lifetimes of dyes in thin PVA layers

Jord C. Prangsma; Robert Molenaar; Vinod Subramaniam; Christian Blum

doi:10.1117/12.2190521

Photonic effects on the fluorescence lifetimes of dyes in thin PVA layers

Jord C. Prangsma, Robert Molenaar, Vinod Subramaniam, Christian Blum

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

In this paper we investigate the expected change in fluorescent decay rate when a fluorophore in aqueous solution is moved to a thin spin-coated layer of poly(vinyl alcohol). We take into account the local field effect due to the change in the refractive index of the medium around the fluorophore and the photonic effect due to the layers. The obtained results are compared with experimental results for the organic dye Atto565 and the fluorescent protein mCherry. We find that the effects for the organic dye can be well described with the model, for the fluorescent protein (FP) the model is less accurate. We discuss the possible explanations for this.

Original language	English
Title of host publication	Nanoimaging and Nanospectroscopy III
Publisher	SPIE
Volume	9554
ISBN (Electronic)	9781628417203
DOIs	https://doi.org/10.1117/12.2190521
Publication status	Published - 26 Aug 2015
Event	Nanoimaging and Nanospectroscopy III - San Diego, United States Duration: 9 Aug 2015 → 12 Aug 2015

Conference

Conference	Nanoimaging and Nanospectroscopy III
Country/Territory	United States
City	San Diego
Period	9/08/15 → 12/08/15

Keywords

fluorescent protein
local density of states
local field effect
PVA

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10.1117/12.2190521

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@inproceedings{90ef724cf01c4adfb7d26a75f04bea7a,

title = "Photonic effects on the fluorescence lifetimes of dyes in thin PVA layers",

abstract = "In this paper we investigate the expected change in fluorescent decay rate when a fluorophore in aqueous solution is moved to a thin spin-coated layer of poly(vinyl alcohol). We take into account the local field effect due to the change in the refractive index of the medium around the fluorophore and the photonic effect due to the layers. The obtained results are compared with experimental results for the organic dye Atto565 and the fluorescent protein mCherry. We find that the effects for the organic dye can be well described with the model, for the fluorescent protein (FP) the model is less accurate. We discuss the possible explanations for this.",

keywords = "fluorescent protein, local density of states, local field effect, PVA",

author = "Prangsma, {Jord C.} and Robert Molenaar and Vinod Subramaniam and Christian Blum",

year = "2015",

month = aug,

day = "26",

doi = "10.1117/12.2190521",

language = "English",

volume = "9554",

booktitle = "Nanoimaging and Nanospectroscopy III",

publisher = "SPIE",

address = "United States",

note = "Nanoimaging and Nanospectroscopy III ; Conference date: 09-08-2015 Through 12-08-2015",

}

TY - GEN

T1 - Photonic effects on the fluorescence lifetimes of dyes in thin PVA layers

AU - Prangsma, Jord C.

AU - Molenaar, Robert

AU - Subramaniam, Vinod

AU - Blum, Christian

PY - 2015/8/26

Y1 - 2015/8/26

N2 - In this paper we investigate the expected change in fluorescent decay rate when a fluorophore in aqueous solution is moved to a thin spin-coated layer of poly(vinyl alcohol). We take into account the local field effect due to the change in the refractive index of the medium around the fluorophore and the photonic effect due to the layers. The obtained results are compared with experimental results for the organic dye Atto565 and the fluorescent protein mCherry. We find that the effects for the organic dye can be well described with the model, for the fluorescent protein (FP) the model is less accurate. We discuss the possible explanations for this.

AB - In this paper we investigate the expected change in fluorescent decay rate when a fluorophore in aqueous solution is moved to a thin spin-coated layer of poly(vinyl alcohol). We take into account the local field effect due to the change in the refractive index of the medium around the fluorophore and the photonic effect due to the layers. The obtained results are compared with experimental results for the organic dye Atto565 and the fluorescent protein mCherry. We find that the effects for the organic dye can be well described with the model, for the fluorescent protein (FP) the model is less accurate. We discuss the possible explanations for this.

KW - fluorescent protein

KW - local density of states

KW - local field effect

KW - PVA

UR - http://www.scopus.com/inward/record.url?scp=84951839451&partnerID=8YFLogxK

U2 - 10.1117/12.2190521

DO - 10.1117/12.2190521

M3 - Conference contribution

AN - SCOPUS:84951839451

VL - 9554

BT - Nanoimaging and Nanospectroscopy III

PB - SPIE

T2 - Nanoimaging and Nanospectroscopy III

Y2 - 9 August 2015 through 12 August 2015

ER -

Photonic effects on the fluorescence lifetimes of dyes in thin PVA layers

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