Photonic emitter manipulation to sample nanoscale topography

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Abstract

We demonstrate that photonic emitter manipulation can be used to image the nanoscale topography of a fluorescently labeled layer in confocal imaging. We exploit the fact that a metallic probe manipulates a fluorophore’s photonic environment, and thereby its fluorescent lifetime, in a strongly distance-dependent manner. To image surface topography, a metallic probe that is not in contact with the surface is rasterscanned over a fluorescently labeled sample. The axial position of the probe is kept constant. At each lateral probe position, the fluorescence decay is recorded and analyzed to obtain probe – sample distances and hence, the topography of the sample. We present images resolving a microfabricated step of 14 nm in topography, with the probe positioned at different axial positions.

Original languageEnglish
Pages (from-to)11698-11708
Number of pages11
JournalOptics express
Volume27
Issue number8
DOIs
Publication statusPublished - 15 Apr 2019

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manipulators
topography
emitters
photonics
probes
life (durability)
fluorescence
decay

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title = "Photonic emitter manipulation to sample nanoscale topography",
abstract = "We demonstrate that photonic emitter manipulation can be used to image the nanoscale topography of a fluorescently labeled layer in confocal imaging. We exploit the fact that a metallic probe manipulates a fluorophore’s photonic environment, and thereby its fluorescent lifetime, in a strongly distance-dependent manner. To image surface topography, a metallic probe that is not in contact with the surface is rasterscanned over a fluorescently labeled sample. The axial position of the probe is kept constant. At each lateral probe position, the fluorescence decay is recorded and analyzed to obtain probe – sample distances and hence, the topography of the sample. We present images resolving a microfabricated step of 14 nm in topography, with the probe positioned at different axial positions.",
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Photonic emitter manipulation to sample nanoscale topography. / Mateos, N.; Molenaar, R.; Claessens, M. M.A.E.; Blum, C.

In: Optics express, Vol. 27, No. 8, 15.04.2019, p. 11698-11708.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Mateos, N.

AU - Molenaar, R.

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AB - We demonstrate that photonic emitter manipulation can be used to image the nanoscale topography of a fluorescently labeled layer in confocal imaging. We exploit the fact that a metallic probe manipulates a fluorophore’s photonic environment, and thereby its fluorescent lifetime, in a strongly distance-dependent manner. To image surface topography, a metallic probe that is not in contact with the surface is rasterscanned over a fluorescently labeled sample. The axial position of the probe is kept constant. At each lateral probe position, the fluorescence decay is recorded and analyzed to obtain probe – sample distances and hence, the topography of the sample. We present images resolving a microfabricated step of 14 nm in topography, with the probe positioned at different axial positions.

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