Photosynthesis in a different light: Spectro-microscopy for in vivo characterization of chloroplasts

Sébastien Peter, Martina B. Zell, Christian Blum, Alexander Stuhl, Kirstin Elgass, Marcus Sackrow, Vinod Subramaniam, Alfred J. Meixner, Klaus Harter, Veronica G. Maurino, Frank E. Schleifenbaum

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

During photosynthesis, energy conversion at the two photosystems is controlled by highly complex and dynamic adaptation processes triggered by external factors such as light quality, intensity, and duration, or internal cues such as carbon availability. These dynamics have remained largely concealed so far, because current analytical techniques are based on the investigation of isolated chloroplasts lacking full adaptation ability and are performed at non-physiologically low temperatures. Here, we use non-invasive in planta spectro-microscopic approaches to investigate living chloroplasts in their native environment at ambient temperatures. This is a valuable approach to study the complex function of these systems, because an intrinsic property-the fluorescence emission-is exploited and no additional external perturbations are introduced. Our analysis demonstrates a dynamic adjustment of not only the photosystemI/photosystemII (PSI/PSII) intensity ratio in the chloroplasts but also of the capacity of the LHCs for energy transfer in response to environmental and internal cues.

Original languageEnglish
Article number292
JournalFrontiers in Plant Science
Volume5
Issue numberJUN
DOIs
Publication statusPublished - 30 Jun 2014

Fingerprint

microscopy
chloroplasts
photosynthesis
energy conversion
light quality
energy transfer
Plantae
analytical methods
ambient temperature
fluorescence
duration
carbon
temperature

Keywords

  • Chloroplast
  • Fluorescence
  • Photochemistry
  • Photosystem
  • Spectromicroscopy
  • Spectroscopy

Cite this

Peter, Sébastien ; Zell, Martina B. ; Blum, Christian ; Stuhl, Alexander ; Elgass, Kirstin ; Sackrow, Marcus ; Subramaniam, Vinod ; Meixner, Alfred J. ; Harter, Klaus ; Maurino, Veronica G. ; Schleifenbaum, Frank E. / Photosynthesis in a different light : Spectro-microscopy for in vivo characterization of chloroplasts. In: Frontiers in Plant Science. 2014 ; Vol. 5, No. JUN.
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Peter, S, Zell, MB, Blum, C, Stuhl, A, Elgass, K, Sackrow, M, Subramaniam, V, Meixner, AJ, Harter, K, Maurino, VG & Schleifenbaum, FE 2014, 'Photosynthesis in a different light: Spectro-microscopy for in vivo characterization of chloroplasts', Frontiers in Plant Science, vol. 5, no. JUN, 292. https://doi.org/10.3389/fpls.2014.00292

Photosynthesis in a different light : Spectro-microscopy for in vivo characterization of chloroplasts. / Peter, Sébastien; Zell, Martina B.; Blum, Christian; Stuhl, Alexander; Elgass, Kirstin; Sackrow, Marcus; Subramaniam, Vinod; Meixner, Alfred J.; Harter, Klaus; Maurino, Veronica G.; Schleifenbaum, Frank E.

In: Frontiers in Plant Science, Vol. 5, No. JUN, 292, 30.06.2014.

Research output: Contribution to journalArticleAcademicpeer-review

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T2 - Spectro-microscopy for in vivo characterization of chloroplasts

AU - Peter, Sébastien

AU - Zell, Martina B.

AU - Blum, Christian

AU - Stuhl, Alexander

AU - Elgass, Kirstin

AU - Sackrow, Marcus

AU - Subramaniam, Vinod

AU - Meixner, Alfred J.

AU - Harter, Klaus

AU - Maurino, Veronica G.

AU - Schleifenbaum, Frank E.

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