Physical discrimination between human T-lymphocyte subpopulations by means of light scattering, revealing two populations of T8-positive cells

Leonardus Wendelinus Mathias Marie Terstappen, B.G. de Grooth, G.M.J. Nolten, C.H.H. ten Napel, W. van Berkel, Jan Greve

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Abstract

Light-scattering properties of human T-lymphocyte subpopulations selected by immunofluorescence were studied. Based on differences in orthogonal light scattering, two subpopulations of T8-positive cells can be distinguished. The first population (T8a) has the same orthogonal light-scattering properties as T4-positive cells, whereas the orthogonal light scattering of the second population (T8b) was about 70% larger. Orthogonal light scattering of Leu7-positive lymphocytes resembles that of the T8b population. We have studied the occurrence of the subpopulation in healthy individuals and we discuss their possible functional identification. Light-scattering properties of lymphocyte subpopulations in two patients with B-cell chronic lymphatic leukemia suggest that this observation is of clinical interest.
Original languageUndefined
Pages (from-to)178-183
JournalCytometry
Volume7
Issue number2
DOIs
Publication statusPublished - 1986

Keywords

  • Flow cytometry
  • IR-60748
  • T-lymphocyte subpopulations
  • Light scattering

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