Physical discrimination between human T-lymphocyte subpopulations by means of light scattering, revealing two populations of T8-positive cells

Leonardus Wendelinus Mathias Marie Terstappen; B.G. de Grooth; G.M.J. Nolten; C.H.H. ten Napel; W. van Berkel; Jan Greve

doi:10.1002/cyto.990070209

Physical discrimination between human T-lymphocyte subpopulations by means of light scattering, revealing two populations of T8-positive cells

Leonardus Wendelinus Mathias Marie Terstappen, B.G. de Grooth, G.M.J. Nolten, C.H.H. ten Napel, W. van Berkel, Jan Greve

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Abstract

Light-scattering properties of human T-lymphocyte subpopulations selected by immunofluorescence were studied. Based on differences in orthogonal light scattering, two subpopulations of T8-positive cells can be distinguished. The first population (T8a) has the same orthogonal light-scattering properties as T4-positive cells, whereas the orthogonal light scattering of the second population (T8b) was about 70% larger. Orthogonal light scattering of Leu7-positive lymphocytes resembles that of the T8b population. We have studied the occurrence of the subpopulation in healthy individuals and we discuss their possible functional identification. Light-scattering properties of lymphocyte subpopulations in two patients with B-cell chronic lymphatic leukemia suggest that this observation is of clinical interest.

Original language	Undefined
Pages (from-to)	178-183
Journal	Cytometry
Volume	7
Issue number	2
DOIs	https://doi.org/10.1002/cyto.990070209
Publication status	Published - 1986

Keywords

Flow cytometry
IR-60748
T-lymphocyte subpopulations
Light scattering

Access to Document

10.1002/cyto.990070209

Terstappen86physical

Cite this

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title = "Physical discrimination between human T-lymphocyte subpopulations by means of light scattering, revealing two populations of T8-positive cells",

abstract = "Light-scattering properties of human T-lymphocyte subpopulations selected by immunofluorescence were studied. Based on differences in orthogonal light scattering, two subpopulations of T8-positive cells can be distinguished. The first population (T8a) has the same orthogonal light-scattering properties as T4-positive cells, whereas the orthogonal light scattering of the second population (T8b) was about 70% larger. Orthogonal light scattering of Leu7-positive lymphocytes resembles that of the T8b population. We have studied the occurrence of the subpopulation in healthy individuals and we discuss their possible functional identification. Light-scattering properties of lymphocyte subpopulations in two patients with B-cell chronic lymphatic leukemia suggest that this observation is of clinical interest.",

keywords = "Flow cytometry, IR-60748, T-lymphocyte subpopulations, Light scattering",

author = "Terstappen, {Leonardus Wendelinus Mathias Marie} and {de Grooth}, B.G. and G.M.J. Nolten and {ten Napel}, C.H.H. and {van Berkel}, W. and Jan Greve",

year = "1986",

doi = "10.1002/cyto.990070209",

language = "Undefined",

volume = "7",

pages = "178--183",

journal = "Cytometry",

issn = "0196-4763",

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number = "2",

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TY - JOUR

T1 - Physical discrimination between human T-lymphocyte subpopulations by means of light scattering, revealing two populations of T8-positive cells

AU - Terstappen, Leonardus Wendelinus Mathias Marie

AU - de Grooth, B.G.

AU - Nolten, G.M.J.

AU - ten Napel, C.H.H.

AU - van Berkel, W.

AU - Greve, Jan

PY - 1986

Y1 - 1986

N2 - Light-scattering properties of human T-lymphocyte subpopulations selected by immunofluorescence were studied. Based on differences in orthogonal light scattering, two subpopulations of T8-positive cells can be distinguished. The first population (T8a) has the same orthogonal light-scattering properties as T4-positive cells, whereas the orthogonal light scattering of the second population (T8b) was about 70% larger. Orthogonal light scattering of Leu7-positive lymphocytes resembles that of the T8b population. We have studied the occurrence of the subpopulation in healthy individuals and we discuss their possible functional identification. Light-scattering properties of lymphocyte subpopulations in two patients with B-cell chronic lymphatic leukemia suggest that this observation is of clinical interest.

AB - Light-scattering properties of human T-lymphocyte subpopulations selected by immunofluorescence were studied. Based on differences in orthogonal light scattering, two subpopulations of T8-positive cells can be distinguished. The first population (T8a) has the same orthogonal light-scattering properties as T4-positive cells, whereas the orthogonal light scattering of the second population (T8b) was about 70% larger. Orthogonal light scattering of Leu7-positive lymphocytes resembles that of the T8b population. We have studied the occurrence of the subpopulation in healthy individuals and we discuss their possible functional identification. Light-scattering properties of lymphocyte subpopulations in two patients with B-cell chronic lymphatic leukemia suggest that this observation is of clinical interest.

KW - Flow cytometry

KW - IR-60748

KW - T-lymphocyte subpopulations

KW - Light scattering

U2 - 10.1002/cyto.990070209

DO - 10.1002/cyto.990070209

M3 - Article

VL - 7

SP - 178

EP - 183

JO - Cytometry

JF - Cytometry

SN - 0196-4763

IS - 2

ER -