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Physical discrimination between human T-lymphocyte subpopulations by means of light scattering, revealing two populations of T8-positive cells

  • L.W.M.M. Terstappen
  • , B.G. de Grooth
  • , G.M.J. Nolten
  • , C.H.H. ten Napel
  • , W. van Berkel
  • , J. Greve

Research output: Contribution to journalArticleAcademic

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Abstract

Light-scattering properties of human T-lymphocyte subpopulations selected by immunofluorescence were studied. Based on differences in orthogonal light scattering, two subpopulations of T8-positive cells can be distinguished. The first population (T8a) has the same orthogonal light-scattering properties as T4-positive cells, whereas the orthogonal light scattering of the second population (T8b) was about 70% larger. Orthogonal light scattering of Leu7-positive lymphocytes resembles that of the T8b population. We have studied the occurrence of the subpopulation in healthy individuals and we discuss their possible functional identification. Light-scattering properties of lymphocyte subpopulations in two patients with B-cell chronic lymphatic leukemia suggest that this observation is of clinical interest.
Original languageEnglish
Pages (from-to)178-183
JournalCytometry
Volume7
Issue number2
DOIs
Publication statusPublished - 1986

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

Keywords

  • Flow cytometry
  • T-lymphocyte subpopulations
  • Light scattering

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