Planar Junctionless Field-Effect Transistor for Detecting Biomolecular Interactions

Rajendra P. Shukla, J. G. Bomer, Daniel Wijnperle, Naveen Kumar, Vihar P. Georgiev, Aruna Chandra Singh, Sivashankar Krishnamoorthy, César Pascual García, Sergii Pud, Wouter Olthuis

Research output: Contribution to journalArticleAcademicpeer-review

6 Downloads (Pure)

Abstract

Label-free field-effect transistor-based immunosensors are promising candidates for proteomics and peptidomics-based diagnostics and therapeutics due to their high multiplexing capability, fast response time, and ability to increase the sensor sensitivity due to the short length of peptides. In this work, planar junctionless field-effect transistor sensors (FETs) were fabricated and characterized for pH sensing. The device with SiO2 gate oxide has shown voltage sensitivity of 41.8 ± 1.4, 39.9 ± 1.4, 39.0 ± 1.1, and 37.6 ± 1.0 mV/pH for constant drain currents of 5, 10, 20, and 50 nA, respectively, with a drain to source voltage of 0.05 V. The drift analysis shows a stability over time of -18 nA/h (pH 7.75), -3.5 nA/h (pH 6.84), -0.5 nA/h (pH 4.91), 0.5 nA/h (pH 3.43), corresponding to a pH drift of -0.45, -0.09, -0.01, and 0.01 per h. Theoretical modeling and simulation resulted in a mean value of the surface states of 3.8 × 1015/cm2 with a standard deviation of 3.6 × 1015/cm2. We have experimentally verified the number of surface sites due to APTES, peptide, and protein immobilization, which is in line with the theoretical calculations for FETs to be used for detecting peptide-protein interactions for future applications.

Original languageEnglish
Article number5783
JournalSensors (Basel, Switzerland)
Volume22
Issue number15
DOIs
Publication statusPublished - 2 Aug 2022

Keywords

  • diagnostics
  • peptide-protein interaction
  • peptidomics
  • pH sensor
  • planar junctionless FETs
  • proteomics
  • therapeutics

Fingerprint

Dive into the research topics of 'Planar Junctionless Field-Effect Transistor for Detecting Biomolecular Interactions'. Together they form a unique fingerprint.

Cite this