Plasmonic Sensing Assay for Long-Term Monitoring (PSALM) of Neurotransmitters in Urine

Wei-Hsin Chen; Wenting Wang; Qianqi Lin; David Benjamin Grys; Marika Niihori; Junyang Huang; Shu Hu; Bart de Nijs; Oren A. Scherman; Jeremy J. Baumberg

doi:10.1021/acsnanoscienceau.2c00048

Plasmonic Sensing Assay for Long-Term Monitoring (PSALM) of Neurotransmitters in Urine

Wei-Hsin Chen, Wenting Wang, Qianqi Lin, David Benjamin Grys, Marika Niihori, Junyang Huang, Shu Hu, Bart de Nijs, Oren A. Scherman, Jeremy J. Baumberg^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A liquid-based surface-enhanced Raman spectroscopy assay termed PSALM is developed for the selective sensing of neurotransmitters (NTs) with a limit of detection below the physiological range of NT concentrations in urine. This assay is formed by quick and simple nanoparticle (NP) "mix-and-measure"protocols, in which FeIII bridges NTs and gold NPs inside the sensing hotspots. Detection limits of NTs from PreNP PSALM are significantly lower than those of PostNP PSALM, when urine is pretreated by affinity separation. Optimized PSALM enables the long-term monitoring of NT variation in urine in conventional settings for the first time, allowing the development of NTs as predictive or correlative biomarkers for clinical diagnosis.

Original language	English
Pages (from-to)	161–171
Journal	ACS Nanoscience Au
Volume	3
Issue number	2
Early online date	24 Dec 2022
DOIs	https://doi.org/10.1021/acsnanoscienceau.2c00048
Publication status	Published - 2023
Externally published	Yes

Keywords

dopamine
neurotransmitters
self-assembly
sensing
SERS
urine

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Chen-2022-Plasmonic-sensing-assay-for-long-teFinal published version, 5.36 MBLicence: CC BY

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title = "Plasmonic Sensing Assay for Long-Term Monitoring (PSALM) of Neurotransmitters in Urine",

abstract = "A liquid-based surface-enhanced Raman spectroscopy assay termed PSALM is developed for the selective sensing of neurotransmitters (NTs) with a limit of detection below the physiological range of NT concentrations in urine. This assay is formed by quick and simple nanoparticle (NP) {"}mix-and-measure{"}protocols, in which FeIII bridges NTs and gold NPs inside the sensing hotspots. Detection limits of NTs from PreNP PSALM are significantly lower than those of PostNP PSALM, when urine is pretreated by affinity separation. Optimized PSALM enables the long-term monitoring of NT variation in urine in conventional settings for the first time, allowing the development of NTs as predictive or correlative biomarkers for clinical diagnosis.",

keywords = "dopamine, neurotransmitters, self-assembly, sensing, SERS, urine",

author = "Wei-Hsin Chen and Wenting Wang and Qianqi Lin and Grys, {David Benjamin} and Marika Niihori and Junyang Huang and Shu Hu and {de Nijs}, Bart and Scherman, {Oren A.} and Baumberg, {Jeremy J.}",

note = "Funding Information: The authors acknowledge financial support from EPSRC Grants EP/L027151/1, EP/L015889/1, and EP/R020965/1 and the Cambridge IAA account. W.H.C. acknowledges support from Mursla and B.d.N. acknowledges support from the Royal Society (URF/R1/211162). The authors greatly appreciate useful discussions with Mursla, LGC, and BBI. M.N. was supported by a Gates Cambridge fellowship (OPP1144). Publisher Copyright: {\textcopyright} 2022 The Authors. Published by American Chemical Society.",

year = "2023",

doi = "10.1021/acsnanoscienceau.2c00048",

language = "English",

volume = "3",

pages = "161–171",

journal = "ACS Nanoscience Au",

issn = "2694-2496",

publisher = "American Chemical Society",

number = "2",

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T1 - Plasmonic Sensing Assay for Long-Term Monitoring (PSALM) of Neurotransmitters in Urine

AU - Chen, Wei-Hsin

AU - Wang, Wenting

AU - Lin, Qianqi

AU - Grys, David Benjamin

AU - Niihori, Marika

AU - Huang, Junyang

AU - Hu, Shu

AU - de Nijs, Bart

AU - Scherman, Oren A.

AU - Baumberg, Jeremy J.

N1 - Funding Information: The authors acknowledge financial support from EPSRC Grants EP/L027151/1, EP/L015889/1, and EP/R020965/1 and the Cambridge IAA account. W.H.C. acknowledges support from Mursla and B.d.N. acknowledges support from the Royal Society (URF/R1/211162). The authors greatly appreciate useful discussions with Mursla, LGC, and BBI. M.N. was supported by a Gates Cambridge fellowship (OPP1144). Publisher Copyright: © 2022 The Authors. Published by American Chemical Society.

PY - 2023

Y1 - 2023

N2 - A liquid-based surface-enhanced Raman spectroscopy assay termed PSALM is developed for the selective sensing of neurotransmitters (NTs) with a limit of detection below the physiological range of NT concentrations in urine. This assay is formed by quick and simple nanoparticle (NP) "mix-and-measure"protocols, in which FeIII bridges NTs and gold NPs inside the sensing hotspots. Detection limits of NTs from PreNP PSALM are significantly lower than those of PostNP PSALM, when urine is pretreated by affinity separation. Optimized PSALM enables the long-term monitoring of NT variation in urine in conventional settings for the first time, allowing the development of NTs as predictive or correlative biomarkers for clinical diagnosis.

AB - A liquid-based surface-enhanced Raman spectroscopy assay termed PSALM is developed for the selective sensing of neurotransmitters (NTs) with a limit of detection below the physiological range of NT concentrations in urine. This assay is formed by quick and simple nanoparticle (NP) "mix-and-measure"protocols, in which FeIII bridges NTs and gold NPs inside the sensing hotspots. Detection limits of NTs from PreNP PSALM are significantly lower than those of PostNP PSALM, when urine is pretreated by affinity separation. Optimized PSALM enables the long-term monitoring of NT variation in urine in conventional settings for the first time, allowing the development of NTs as predictive or correlative biomarkers for clinical diagnosis.

KW - dopamine

KW - neurotransmitters

KW - self-assembly

KW - sensing

KW - SERS

KW - urine

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JO - ACS Nanoscience Au

JF - ACS Nanoscience Au

IS - 2

ER -

Plasmonic Sensing Assay for Long-Term Monitoring (PSALM) of Neurotransmitters in Urine

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Keywords

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