Platelet deposition in a capillary perfusion model: quantitative and morphological aspects

A. Poot; T. Beugeling; J.P. Cazenave; A. Bantjes; W.G. van Aken

doi:10.1016/0142-9612(88)90110-X

Platelet deposition in a capillary perfusion model: quantitative and morphological aspects

A. Poot, T. Beugeling, J.P. Cazenave, A. Bantjes, W.G. van Aken

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Abstract

The capillary perfusion model according to Cazenave and co-workers was characterized by investigatingium labelling of human platelets and scanning electron microscopy (SEM). Compared with uncoated polyethylene, platelet deposition was increased after precoating with purified human von Willebrand factor, fibrinogen or fibronectin, and decreased by preadsorbed immunoglobulin G, albumin or whole plasma. Platelet aggregates were observed on immunoglobulin G-coated polyethylene, whereas all other surfaces showed single adherent platelets. Complete platelet spreading was only observed after precoating with fibronectin. The quantitative data concerning platelet deposition were evaluated by using the convective-diffusion theory. Our results indicate the applicability of this perfusion model for the in vitro testing of biomaterials.

Original language	English
Pages (from-to)	126-132
Journal	Biomaterials
Volume	9
Issue number	2
DOIs	https://doi.org/10.1016/0142-9612(88)90110-X
Publication status	Published - 1988

Keywords

IR-70302
washed platelets
platelet deposition
perfusion model
Protein adsorption
Blood

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title = "Platelet deposition in a capillary perfusion model: quantitative and morphological aspects",

abstract = "The capillary perfusion model according to Cazenave and co-workers was characterized by investigatingium labelling of human platelets and scanning electron microscopy (SEM). Compared with uncoated polyethylene, platelet deposition was increased after precoating with purified human von Willebrand factor, fibrinogen or fibronectin, and decreased by preadsorbed immunoglobulin G, albumin or whole plasma. Platelet aggregates were observed on immunoglobulin G-coated polyethylene, whereas all other surfaces showed single adherent platelets. Complete platelet spreading was only observed after precoating with fibronectin. The quantitative data concerning platelet deposition were evaluated by using the convective-diffusion theory. Our results indicate the applicability of this perfusion model for the in vitro testing of biomaterials.",

keywords = "IR-70302, washed platelets, platelet deposition, perfusion model, Protein adsorption, Blood",

author = "A. Poot and T. Beugeling and J.P. Cazenave and A. Bantjes and {van Aken}, W.G.",

year = "1988",

doi = "10.1016/0142-9612(88)90110-X",

language = "English",

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pages = "126--132",

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T1 - Platelet deposition in a capillary perfusion model: quantitative and morphological aspects

AU - Poot, A.

AU - Beugeling, T.

AU - Cazenave, J.P.

AU - Bantjes, A.

AU - van Aken, W.G.

PY - 1988

Y1 - 1988

N2 - The capillary perfusion model according to Cazenave and co-workers was characterized by investigatingium labelling of human platelets and scanning electron microscopy (SEM). Compared with uncoated polyethylene, platelet deposition was increased after precoating with purified human von Willebrand factor, fibrinogen or fibronectin, and decreased by preadsorbed immunoglobulin G, albumin or whole plasma. Platelet aggregates were observed on immunoglobulin G-coated polyethylene, whereas all other surfaces showed single adherent platelets. Complete platelet spreading was only observed after precoating with fibronectin. The quantitative data concerning platelet deposition were evaluated by using the convective-diffusion theory. Our results indicate the applicability of this perfusion model for the in vitro testing of biomaterials.

AB - The capillary perfusion model according to Cazenave and co-workers was characterized by investigatingium labelling of human platelets and scanning electron microscopy (SEM). Compared with uncoated polyethylene, platelet deposition was increased after precoating with purified human von Willebrand factor, fibrinogen or fibronectin, and decreased by preadsorbed immunoglobulin G, albumin or whole plasma. Platelet aggregates were observed on immunoglobulin G-coated polyethylene, whereas all other surfaces showed single adherent platelets. Complete platelet spreading was only observed after precoating with fibronectin. The quantitative data concerning platelet deposition were evaluated by using the convective-diffusion theory. Our results indicate the applicability of this perfusion model for the in vitro testing of biomaterials.

KW - IR-70302

KW - washed platelets

KW - platelet deposition

KW - perfusion model

KW - Protein adsorption

KW - Blood

U2 - 10.1016/0142-9612(88)90110-X

DO - 10.1016/0142-9612(88)90110-X

M3 - Article

VL - 9

SP - 126

EP - 132

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 2

ER -

Platelet deposition in a capillary perfusion model: quantitative and morphological aspects

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Keywords

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