Platelet deposition in a capillary perfusion model: quantitative and morphological aspects

A. Poot, T. Beugeling, J.P. Cazenave, A. Bantjes, W.G. van Aken

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Abstract

The capillary perfusion model according to Cazenave and co-workers was characterized by investigatingium labelling of human platelets and scanning electron microscopy (SEM). Compared with uncoated polyethylene, platelet deposition was increased after precoating with purified human von Willebrand factor, fibrinogen or fibronectin, and decreased by preadsorbed immunoglobulin G, albumin or whole plasma. Platelet aggregates were observed on immunoglobulin G-coated polyethylene, whereas all other surfaces showed single adherent platelets. Complete platelet spreading was only observed after precoating with fibronectin. The quantitative data concerning platelet deposition were evaluated by using the convective-diffusion theory. Our results indicate the applicability of this perfusion model for the in vitro testing of biomaterials.
Original languageEnglish
Pages (from-to)126-132
JournalBiomaterials
Volume9
Issue number2
DOIs
Publication statusPublished - 1988

Keywords

  • IR-70302
  • washed platelets
  • platelet deposition
  • perfusion model
  • Protein adsorption
  • Blood

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