Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities

Jeanne Elisabeth van Dongen*, Johanna Theodora Wilhelmina Berendsen, Renske D.M. Steenbergen, Rob M. F. Wolthuis, Jan C.T. Eijkel, Loes Segerink

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)
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Abstract

With the trend of moving molecular tests from clinical diagnostic laboratories to on-site testing, there is a need for nucleic acid based diagnostic tools combining the sensitivity, specificity and flexibility of established genetic diagnostics with the ease, cost effectiveness and speed of isothermal amplification and detection methods.

A promising new nucleic acid detection method is Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated nuclease (Cas)-based sensing. In this method Cas effector proteins are used as highly specific sequence recognition elements that can be combined with many different read-out methods for on-site point-of-care testing.

This review covers the technical aspects of integrating CRISPR/Cas technology in miniaturized sensors for analysis on-site. We start with a short introduction to CRISPR/Cas systems and the different effector proteins and continue with reviewing the recent developments of integrating CRISPR sensing in miniaturized sensors for point-of-care applications. Finally, we discuss the challenges of point-of-care CRISPR sensing and describe future research perspectives.
Original languageEnglish
Article number112445
Number of pages17
JournalBiosensors and bioelectronics
Volume166
Issue number112445
DOIs
Publication statusPublished - 15 Oct 2020

Keywords

  • point-of-care
  • CRISPR/Cas
  • DNA sensing
  • Genomic diagnostic tools
  • CRISPR sensing
  • Diagnosis

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