Polarization-sensitive coherent anti-Stokes Raman scattering (PSCARS) is used to investigate the secondary structure of the protein chymotrypsin, both free and bound to antranilic acid. Advantage is taken of the extreme sensitivity of the PSCARS spectra to the orientation of the analyser. Clear changes are observed in the protein spectra as a result of binding to antranilic acid. It is concluded that PSCARS can be fruitfully applied to detect changes in the bonds of the peptide backbone of enzymatic proteins as a result of substrate binding.
|Number of pages||4|
|Journal||Journal of raman spectroscopy|
|Publication status||Published - 1996|