Poly(Amido Amine)s containing agmatine and butanol side chains as efficient gene carriers

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

A new type of bioreducible poly(amido amine) copolymer is synthesized by the Michael addition polymerization of cystamine bisacrylamide (CBA) with 4-aminobutylguanidine (agmatine, AGM) and 4-aminobutanol (ABOL). Since the positively charged guanidinium groups of AGM and the hydroxybutyl groups of ABOL in the side chains have shown to improve the overall transfection efficiency of poly(amido amine)s, it is hypothesized that poly(CBA-ABOL/AGM) synthesized at the optimal ratio of both components would result in high transfection efficiency and minimal toxicity. In this study, a series of the poly(CBA-ABOL/AGM) copolymers is synthesized as gene carriers. The polymers are characterized and luciferase transfection efficiencies of the polymers in various cell lines are investigated to select the ideal ratio between AGM and ABOL. The poly(CBA-ABOL/AGM) containing 80% AGM and 20% ABOL has shown the best transfection efficiency with the lowest cytotoxicity, indicating that this polymer is very promising as a potent and nontoxic gene carrier
Original languageEnglish
Pages (from-to)619-626
JournalMacromolecular bioscience
Volume16
Issue number4
DOIs
Publication statusPublished - 11 Dec 2016

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Agmatine
Butanols
Butenes
Amines
Cystamine
Genes
Transfection
Polymers
Copolymers
Cytotoxicity
Toxicity
Polymerization
Cells
Guanidine
4-aminobutanol
Luciferases
Cell Line

Keywords

  • IR-99629
  • METIS-315331

Cite this

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title = "Poly(Amido Amine)s containing agmatine and butanol side chains as efficient gene carriers",
abstract = "A new type of bioreducible poly(amido amine) copolymer is synthesized by the Michael addition polymerization of cystamine bisacrylamide (CBA) with 4-aminobutylguanidine (agmatine, AGM) and 4-aminobutanol (ABOL). Since the positively charged guanidinium groups of AGM and the hydroxybutyl groups of ABOL in the side chains have shown to improve the overall transfection efficiency of poly(amido amine)s, it is hypothesized that poly(CBA-ABOL/AGM) synthesized at the optimal ratio of both components would result in high transfection efficiency and minimal toxicity. In this study, a series of the poly(CBA-ABOL/AGM) copolymers is synthesized as gene carriers. The polymers are characterized and luciferase transfection efficiencies of the polymers in various cell lines are investigated to select the ideal ratio between AGM and ABOL. The poly(CBA-ABOL/AGM) containing 80{\%} AGM and 20{\%} ABOL has shown the best transfection efficiency with the lowest cytotoxicity, indicating that this polymer is very promising as a potent and nontoxic gene carrier",
keywords = "IR-99629, METIS-315331",
author = "Young-Wook Won and Ankone, {Martinus J.K.} and Engbersen, {Johannes F.J.} and Jan Feijen and S.W. Kim",
year = "2016",
month = "12",
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doi = "10.1002/mabi.201500369",
language = "English",
volume = "16",
pages = "619--626",
journal = "Macromolecular bioscience",
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Poly(Amido Amine)s containing agmatine and butanol side chains as efficient gene carriers. / Won, Young-Wook; Ankone, Martinus J.K.; Engbersen, Johannes F.J.; Feijen, Jan; Kim, S.W.

In: Macromolecular bioscience, Vol. 16, No. 4, 11.12.2016, p. 619-626.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Poly(Amido Amine)s containing agmatine and butanol side chains as efficient gene carriers

AU - Won, Young-Wook

AU - Ankone, Martinus J.K.

AU - Engbersen, Johannes F.J.

AU - Feijen, Jan

AU - Kim, S.W.

PY - 2016/12/11

Y1 - 2016/12/11

N2 - A new type of bioreducible poly(amido amine) copolymer is synthesized by the Michael addition polymerization of cystamine bisacrylamide (CBA) with 4-aminobutylguanidine (agmatine, AGM) and 4-aminobutanol (ABOL). Since the positively charged guanidinium groups of AGM and the hydroxybutyl groups of ABOL in the side chains have shown to improve the overall transfection efficiency of poly(amido amine)s, it is hypothesized that poly(CBA-ABOL/AGM) synthesized at the optimal ratio of both components would result in high transfection efficiency and minimal toxicity. In this study, a series of the poly(CBA-ABOL/AGM) copolymers is synthesized as gene carriers. The polymers are characterized and luciferase transfection efficiencies of the polymers in various cell lines are investigated to select the ideal ratio between AGM and ABOL. The poly(CBA-ABOL/AGM) containing 80% AGM and 20% ABOL has shown the best transfection efficiency with the lowest cytotoxicity, indicating that this polymer is very promising as a potent and nontoxic gene carrier

AB - A new type of bioreducible poly(amido amine) copolymer is synthesized by the Michael addition polymerization of cystamine bisacrylamide (CBA) with 4-aminobutylguanidine (agmatine, AGM) and 4-aminobutanol (ABOL). Since the positively charged guanidinium groups of AGM and the hydroxybutyl groups of ABOL in the side chains have shown to improve the overall transfection efficiency of poly(amido amine)s, it is hypothesized that poly(CBA-ABOL/AGM) synthesized at the optimal ratio of both components would result in high transfection efficiency and minimal toxicity. In this study, a series of the poly(CBA-ABOL/AGM) copolymers is synthesized as gene carriers. The polymers are characterized and luciferase transfection efficiencies of the polymers in various cell lines are investigated to select the ideal ratio between AGM and ABOL. The poly(CBA-ABOL/AGM) containing 80% AGM and 20% ABOL has shown the best transfection efficiency with the lowest cytotoxicity, indicating that this polymer is very promising as a potent and nontoxic gene carrier

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