Polyethylene glycol-grafted polystyrene particles

Fenghua Meng; G.H.M. Engbers; Jan Feijen

doi:10.1002/jbm.a.30056

Polyethylene glycol-grafted polystyrene particles

Fenghua Meng, G.H.M. Engbers, Jan Feijen

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Densely pegylated particles that can serve as a model system for artificial cells were prepared by covalently grafting amino polyethylene glycol (PEG, molecular weight 3400 or 5000) onto carboxyl polystyrene particles (PS-COOH) using carbodiimide chemistry. PEG-modified particles (PS-PEG) were characterized by determination of the PEG surface concentration, -potential, size, and morphology. Under optimized grafting conditions, a dense brush-like PEG layer was formed. A PEG surface concentration of approximately 60 pmol/cm2, corresponding with an average distance between grafted PEG chains of 17 Å can be realized. It was shown that grafting of PEG onto PS-COOH reduced the adsorption of proteins from human plasma (85 vol %) in phosphate-buffered saline up to 90%

Original language	Undefined
Pages (from-to)	49-58
Journal	Journal of biomedical materials research. Part A
Volume	70A
Issue number	1
DOIs	https://doi.org/10.1002/jbm.a.30056
Publication status	Published - 2004

Keywords

IR-71380
METIS-223580

Access to Document

10.1002/jbm.a.30056

Cite this

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title = "Polyethylene glycol-grafted polystyrene particles",

abstract = "Densely pegylated particles that can serve as a model system for artificial cells were prepared by covalently grafting amino polyethylene glycol (PEG, molecular weight 3400 or 5000) onto carboxyl polystyrene particles (PS-COOH) using carbodiimide chemistry. PEG-modified particles (PS-PEG) were characterized by determination of the PEG surface concentration, -potential, size, and morphology. Under optimized grafting conditions, a dense brush-like PEG layer was formed. A PEG surface concentration of approximately 60 pmol/cm2, corresponding with an average distance between grafted PEG chains of 17 {\AA} can be realized. It was shown that grafting of PEG onto PS-COOH reduced the adsorption of proteins from human plasma (85 vol %) in phosphate-buffered saline up to 90%",

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T1 - Polyethylene glycol-grafted polystyrene particles

AU - Meng, Fenghua

AU - Engbers, G.H.M.

AU - Feijen, Jan

PY - 2004

Y1 - 2004

N2 - Densely pegylated particles that can serve as a model system for artificial cells were prepared by covalently grafting amino polyethylene glycol (PEG, molecular weight 3400 or 5000) onto carboxyl polystyrene particles (PS-COOH) using carbodiimide chemistry. PEG-modified particles (PS-PEG) were characterized by determination of the PEG surface concentration, -potential, size, and morphology. Under optimized grafting conditions, a dense brush-like PEG layer was formed. A PEG surface concentration of approximately 60 pmol/cm2, corresponding with an average distance between grafted PEG chains of 17 Å can be realized. It was shown that grafting of PEG onto PS-COOH reduced the adsorption of proteins from human plasma (85 vol %) in phosphate-buffered saline up to 90%

AB - Densely pegylated particles that can serve as a model system for artificial cells were prepared by covalently grafting amino polyethylene glycol (PEG, molecular weight 3400 or 5000) onto carboxyl polystyrene particles (PS-COOH) using carbodiimide chemistry. PEG-modified particles (PS-PEG) were characterized by determination of the PEG surface concentration, -potential, size, and morphology. Under optimized grafting conditions, a dense brush-like PEG layer was formed. A PEG surface concentration of approximately 60 pmol/cm2, corresponding with an average distance between grafted PEG chains of 17 Å can be realized. It was shown that grafting of PEG onto PS-COOH reduced the adsorption of proteins from human plasma (85 vol %) in phosphate-buffered saline up to 90%

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KW - METIS-223580

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DO - 10.1002/jbm.a.30056

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JO - Journal of biomedical materials research. Part A

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