Pores in synthetic nerve conduits are beneficial to regeneration

C.L.A.M. Vleggeert-lankamp, G.C.W. de Ruiter, J.F.C. Wolfs, A.P. Pêgo, R.J. van den Berg, H.K.P. Feirabend, M.J.A. Malessy, E.A.J.F. Lakke

Research output: Contribution to journalArticleAcademic

56 Citations (Scopus)

Abstract

Current opinion holds that pores in synthetic nerve guides facilitate nerve regeneration. Solid factual support for this opinion, however, is absent; most of the relevant studies assessed only morphological parameters and results have been contradictory. To evaluate the effect of pores, the rat sciatic nerve was either autografted or grafted with nonporous, macroporous (10-230 m), and microporous (1-10 m) biodegradable -caprolactone grafts. Twelve weeks later, the grafted nerves were resected, and the electrophysiological properties were determined in vitro. Subsequently midgraft-level sections were inspected, and peroneal nerve sections were evaluated morphometrically. Finally, the gastrocnemic and tibial muscle morphometrical properties were quantified. The microporous nerve graft performed much better than the nonporous and macroporous grafts with respect to most parameters: it was bridged by a free floating bundle that contained myelinated nerve fibers, there were more nerve fibers present distal to the graft, the electrophysiological response rate was higher, and the decrease in muscle cross-sectional area was markedly smaller. Hence, the present study demonstrates the beneficial effect of synthetic nerve guide pores on nerve regeneration, although with the caveat that not pores per se, but only small (1-10 m) pores were effective.
Original languageUndefined
Pages (from-to)965-982
JournalJournal of biomedical materials research. Part A
Volume80
Issue number4
DOIs
Publication statusPublished - 2007

Keywords

  • Nerve regeneration
  • Nerve guide
  • IR-72337
  • Porosity
  • nerve morphology
  • Electrophysiology

Cite this

Vleggeert-lankamp, C. L. A. M., de Ruiter, G. C. W., Wolfs, J. F. C., Pêgo, A. P., van den Berg, R. J., Feirabend, H. K. P., ... Lakke, E. A. J. F. (2007). Pores in synthetic nerve conduits are beneficial to regeneration. Journal of biomedical materials research. Part A, 80(4), 965-982. https://doi.org/10.1002/jbm.a.30941
Vleggeert-lankamp, C.L.A.M. ; de Ruiter, G.C.W. ; Wolfs, J.F.C. ; Pêgo, A.P. ; van den Berg, R.J. ; Feirabend, H.K.P. ; Malessy, M.J.A. ; Lakke, E.A.J.F. / Pores in synthetic nerve conduits are beneficial to regeneration. In: Journal of biomedical materials research. Part A. 2007 ; Vol. 80, No. 4. pp. 965-982.
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abstract = "Current opinion holds that pores in synthetic nerve guides facilitate nerve regeneration. Solid factual support for this opinion, however, is absent; most of the relevant studies assessed only morphological parameters and results have been contradictory. To evaluate the effect of pores, the rat sciatic nerve was either autografted or grafted with nonporous, macroporous (10-230 m), and microporous (1-10 m) biodegradable -caprolactone grafts. Twelve weeks later, the grafted nerves were resected, and the electrophysiological properties were determined in vitro. Subsequently midgraft-level sections were inspected, and peroneal nerve sections were evaluated morphometrically. Finally, the gastrocnemic and tibial muscle morphometrical properties were quantified. The microporous nerve graft performed much better than the nonporous and macroporous grafts with respect to most parameters: it was bridged by a free floating bundle that contained myelinated nerve fibers, there were more nerve fibers present distal to the graft, the electrophysiological response rate was higher, and the decrease in muscle cross-sectional area was markedly smaller. Hence, the present study demonstrates the beneficial effect of synthetic nerve guide pores on nerve regeneration, although with the caveat that not pores per se, but only small (1-10 m) pores were effective.",
keywords = "Nerve regeneration, Nerve guide, IR-72337, Porosity, nerve morphology, Electrophysiology",
author = "C.L.A.M. Vleggeert-lankamp and {de Ruiter}, G.C.W. and J.F.C. Wolfs and A.P. P{\^e}go and {van den Berg}, R.J. and H.K.P. Feirabend and M.J.A. Malessy and E.A.J.F. Lakke",
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Vleggeert-lankamp, CLAM, de Ruiter, GCW, Wolfs, JFC, Pêgo, AP, van den Berg, RJ, Feirabend, HKP, Malessy, MJA & Lakke, EAJF 2007, 'Pores in synthetic nerve conduits are beneficial to regeneration' Journal of biomedical materials research. Part A, vol. 80, no. 4, pp. 965-982. https://doi.org/10.1002/jbm.a.30941

Pores in synthetic nerve conduits are beneficial to regeneration. / Vleggeert-lankamp, C.L.A.M.; de Ruiter, G.C.W.; Wolfs, J.F.C.; Pêgo, A.P.; van den Berg, R.J.; Feirabend, H.K.P.; Malessy, M.J.A.; Lakke, E.A.J.F.

In: Journal of biomedical materials research. Part A, Vol. 80, No. 4, 2007, p. 965-982.

Research output: Contribution to journalArticleAcademic

TY - JOUR

T1 - Pores in synthetic nerve conduits are beneficial to regeneration

AU - Vleggeert-lankamp, C.L.A.M.

AU - de Ruiter, G.C.W.

AU - Wolfs, J.F.C.

AU - Pêgo, A.P.

AU - van den Berg, R.J.

AU - Feirabend, H.K.P.

AU - Malessy, M.J.A.

AU - Lakke, E.A.J.F.

PY - 2007

Y1 - 2007

N2 - Current opinion holds that pores in synthetic nerve guides facilitate nerve regeneration. Solid factual support for this opinion, however, is absent; most of the relevant studies assessed only morphological parameters and results have been contradictory. To evaluate the effect of pores, the rat sciatic nerve was either autografted or grafted with nonporous, macroporous (10-230 m), and microporous (1-10 m) biodegradable -caprolactone grafts. Twelve weeks later, the grafted nerves were resected, and the electrophysiological properties were determined in vitro. Subsequently midgraft-level sections were inspected, and peroneal nerve sections were evaluated morphometrically. Finally, the gastrocnemic and tibial muscle morphometrical properties were quantified. The microporous nerve graft performed much better than the nonporous and macroporous grafts with respect to most parameters: it was bridged by a free floating bundle that contained myelinated nerve fibers, there were more nerve fibers present distal to the graft, the electrophysiological response rate was higher, and the decrease in muscle cross-sectional area was markedly smaller. Hence, the present study demonstrates the beneficial effect of synthetic nerve guide pores on nerve regeneration, although with the caveat that not pores per se, but only small (1-10 m) pores were effective.

AB - Current opinion holds that pores in synthetic nerve guides facilitate nerve regeneration. Solid factual support for this opinion, however, is absent; most of the relevant studies assessed only morphological parameters and results have been contradictory. To evaluate the effect of pores, the rat sciatic nerve was either autografted or grafted with nonporous, macroporous (10-230 m), and microporous (1-10 m) biodegradable -caprolactone grafts. Twelve weeks later, the grafted nerves were resected, and the electrophysiological properties were determined in vitro. Subsequently midgraft-level sections were inspected, and peroneal nerve sections were evaluated morphometrically. Finally, the gastrocnemic and tibial muscle morphometrical properties were quantified. The microporous nerve graft performed much better than the nonporous and macroporous grafts with respect to most parameters: it was bridged by a free floating bundle that contained myelinated nerve fibers, there were more nerve fibers present distal to the graft, the electrophysiological response rate was higher, and the decrease in muscle cross-sectional area was markedly smaller. Hence, the present study demonstrates the beneficial effect of synthetic nerve guide pores on nerve regeneration, although with the caveat that not pores per se, but only small (1-10 m) pores were effective.

KW - Nerve regeneration

KW - Nerve guide

KW - IR-72337

KW - Porosity

KW - nerve morphology

KW - Electrophysiology

U2 - 10.1002/jbm.a.30941

DO - 10.1002/jbm.a.30941

M3 - Article

VL - 80

SP - 965

EP - 982

JO - Journal of biomedical materials research. Part A

JF - Journal of biomedical materials research. Part A

SN - 1549-3296

IS - 4

ER -

Vleggeert-lankamp CLAM, de Ruiter GCW, Wolfs JFC, Pêgo AP, van den Berg RJ, Feirabend HKP et al. Pores in synthetic nerve conduits are beneficial to regeneration. Journal of biomedical materials research. Part A. 2007;80(4):965-982. https://doi.org/10.1002/jbm.a.30941