Porous PEOT/PBT scaffolds for bone tissue engineering: preparation, characterization, and in vitro bone marrow cell culturing

Menno B. Claase, Dirk W. Grijpma, Sandra C. Mendes, Joost D. de Bruijn, Jan Feijen

Research output: Contribution to journalArticleAcademicpeer-review

52 Citations (Scopus)

Abstract

The preparation, characterization, and in vitro bone marrow cell culturing on porous PEOT/PBT copolymer scaffolds are described. These scaffolds are meant for use in bone tissue engineering. Previous research has shown that PEOT/PBT copolymers showed in vivo degradation, calcification, and bone bonding. Despite this, several of these copolymers do not support bone marrow cell growth in vitro. Surface modification, such as gas-plasma treatment, is needed to improve the in vitro cell attachment. Porous structures were prepared using a freeze-drying and a salt-leaching technique, the latter one resulting in highly porous interconnected structures of large pore size. Gas-plasma treatment with CO2 generated a surface throughout the entire structure that enabled bone marrow cells to attach. The amount of DNA was determined as a measure for the amount of cells present on the scaffolds. No significant effect of pore size on the amount of DNA present was seen for scaffolds with pore sizes between 250-1000 m. Light microscopy data showed cells in the center of the scaffolds, more cells were observed in the scaffolds of 425-500 m and 500-710 m pore size compared to the ones with 250-425 m and 710-1000 m pores
Original languageEnglish
Pages (from-to)291-300
JournalJournal of biomedical materials research. Part A
Volume64
Issue number2
DOIs
Publication statusPublished - 2003

Keywords

  • IR-71388
  • METIS-210918

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