Portable optoelectronic system for monitoring enzymatic chemiluminescent reaction

F. Costantini; R.M. Tiggelaar; R. Salvio; M. Nardecchia; S. Schlautmann; C. Manetti; H.J.G.E. Gardeniers; D. Caputo; A. Nascetti; G. de Cesare

doi:10.1007/978-3-030-04324-7_25

Portable optoelectronic system for monitoring enzymatic chemiluminescent reaction

F. Costantini, R.M. Tiggelaar, R. Salvio, M. Nardecchia, S. Schlautmann, C. Manetti, H.J.G.E. Gardeniers, D. Caputo^*, A. Nascetti, G. de Cesare

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

This work presents a portable lab-on-chip system, based on thin film electronic devices and an all-glass microfluidic network, for the real-time monitoring of enzymatic chemiluminescent reactions. The microfluidic network is patterned, through wet etching, in a 1.1 mm-thick glass substrate that is subsequently bonded to a 0.5 mm-thick glass substrate. The electronic devices are amorphous silicon p-i-n photosensors, deposited on the outer side of the thinner glass substrate. The photosensors, the microfluidic network and the electronic boards reading out the photodiodes’ current are enclosed in a small metallic box (10×8×15×cm ³ ) in order to ensure shielding from electromagnetic interferences. Preliminary tests have been performed immobilizing horseradish peroxidase on the inner wall of the microchannel as model enzyme for detecting hydrogen peroxide. Limits of detection and quantification equal to 18 and 60 μM, respectively, have been found. These values are comparable to the best performances reported in literature for chemiluminescent-based optofluidic sensors.

Original language	English
Title of host publication	Sensors - Proceedings of the 4th National Conference on Sensors, 2018
Editors	Francesco Baldini, Pietro Siciliano, Marco Rossi, Lorenzo Scalise, Corrado Di Natale, Vittorio Ferrari, Valeria Militello, Giorgia Miolo, Bruno Andò, Vincenzo Marletta, Giovanna Marrazza
Publisher	Springer
Pages	189-194
Number of pages	6
ISBN (Print)	9783030043230
DOIs	https://doi.org/10.1007/978-3-030-04324-7_25
Publication status	Published - 2019
Event	4th National Conference on Sensors, 2018 - Catania, Italy Duration: 21 Feb 2018 → 23 Feb 2018 Conference number: 4

Publication series

Name	Lecture Notes in Electrical Engineering
Volume	539
ISSN (Print)	1876-1100
ISSN (Electronic)	1876-1119

Conference

Conference	4th National Conference on Sensors, 2018
Country/Territory	Italy
City	Catania
Period	21/02/18 → 23/02/18

Keywords

Amorphous silicon
Anodic bonding
Enzymatic reactions
Horseradish peroxidase
Microfluidics
Photosensors

Access to Document

10.1007/978-3-030-04324-7_25

Cite this

Costantini, F., Tiggelaar, R. M., Salvio, R., Nardecchia, M., Schlautmann, S., Manetti, C., Gardeniers, H. J. G. E., Caputo, D., Nascetti, A., & de Cesare, G. (2019). Portable optoelectronic system for monitoring enzymatic chemiluminescent reaction. In F. Baldini, P. Siciliano, M. Rossi, L. Scalise, C. Di Natale, V. Ferrari, V. Militello, G. Miolo, B. Andò, V. Marletta, & G. Marrazza (Eds.), Sensors - Proceedings of the 4th National Conference on Sensors, 2018 (pp. 189-194). (Lecture Notes in Electrical Engineering; Vol. 539). Springer. https://doi.org/10.1007/978-3-030-04324-7_25

Costantini, F. ; Tiggelaar, R.M. ; Salvio, R. et al. / Portable optoelectronic system for monitoring enzymatic chemiluminescent reaction. Sensors - Proceedings of the 4th National Conference on Sensors, 2018. editor / Francesco Baldini ; Pietro Siciliano ; Marco Rossi ; Lorenzo Scalise ; Corrado Di Natale ; Vittorio Ferrari ; Valeria Militello ; Giorgia Miolo ; Bruno Andò ; Vincenzo Marletta ; Giovanna Marrazza. Springer, 2019. pp. 189-194 (Lecture Notes in Electrical Engineering).

@inproceedings{25ad9d8e1de543279f66a0fbcd3e7752,

title = "Portable optoelectronic system for monitoring enzymatic chemiluminescent reaction",

abstract = " This work presents a portable lab-on-chip system, based on thin film electronic devices and an all-glass microfluidic network, for the real-time monitoring of enzymatic chemiluminescent reactions. The microfluidic network is patterned, through wet etching, in a 1.1 mm-thick glass substrate that is subsequently bonded to a 0.5 mm-thick glass substrate. The electronic devices are amorphous silicon p-i-n photosensors, deposited on the outer side of the thinner glass substrate. The photosensors, the microfluidic network and the electronic boards reading out the photodiodes{\textquoteright} current are enclosed in a small metallic box (10×8×15×cm 3 ) in order to ensure shielding from electromagnetic interferences. Preliminary tests have been performed immobilizing horseradish peroxidase on the inner wall of the microchannel as model enzyme for detecting hydrogen peroxide. Limits of detection and quantification equal to 18 and 60 μM, respectively, have been found. These values are comparable to the best performances reported in literature for chemiluminescent-based optofluidic sensors. ",

keywords = "Amorphous silicon, Anodic bonding, Enzymatic reactions, Horseradish peroxidase, Microfluidics, Photosensors",

author = "F. Costantini and R.M. Tiggelaar and R. Salvio and M. Nardecchia and S. Schlautmann and C. Manetti and H.J.G.E. Gardeniers and D. Caputo and A. Nascetti and {de Cesare}, G.",

year = "2019",

doi = "10.1007/978-3-030-04324-7_25",

language = "English",

isbn = "9783030043230",

series = "Lecture Notes in Electrical Engineering",

publisher = "Springer",

pages = "189--194",

editor = "Francesco Baldini and Pietro Siciliano and Marco Rossi and Lorenzo Scalise and {Di Natale}, Corrado and Vittorio Ferrari and Valeria Militello and Giorgia Miolo and Bruno And{\`o} and Vincenzo Marletta and Giovanna Marrazza",

booktitle = "Sensors - Proceedings of the 4th National Conference on Sensors, 2018",

address = "Germany",

note = "4th National Conference on Sensors, 2018 ; Conference date: 21-02-2018 Through 23-02-2018",

}

Costantini, F, Tiggelaar, RM, Salvio, R, Nardecchia, M, Schlautmann, S, Manetti, C, Gardeniers, HJGE, Caputo, D, Nascetti, A & de Cesare, G 2019, Portable optoelectronic system for monitoring enzymatic chemiluminescent reaction. in F Baldini, P Siciliano, M Rossi, L Scalise, C Di Natale, V Ferrari, V Militello, G Miolo, B Andò, V Marletta & G Marrazza (eds), Sensors - Proceedings of the 4th National Conference on Sensors, 2018. Lecture Notes in Electrical Engineering, vol. 539, Springer, pp. 189-194, 4th National Conference on Sensors, 2018, Catania, Italy, 21/02/18. https://doi.org/10.1007/978-3-030-04324-7_25

Portable optoelectronic system for monitoring enzymatic chemiluminescent reaction. / Costantini, F.; Tiggelaar, R.M.; Salvio, R. et al.
Sensors - Proceedings of the 4th National Conference on Sensors, 2018. ed. / Francesco Baldini; Pietro Siciliano; Marco Rossi; Lorenzo Scalise; Corrado Di Natale; Vittorio Ferrari; Valeria Militello; Giorgia Miolo; Bruno Andò; Vincenzo Marletta; Giovanna Marrazza. Springer, 2019. p. 189-194 (Lecture Notes in Electrical Engineering; Vol. 539).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Portable optoelectronic system for monitoring enzymatic chemiluminescent reaction

AU - Costantini, F.

AU - Tiggelaar, R.M.

AU - Salvio, R.

AU - Nardecchia, M.

AU - Schlautmann, S.

AU - Manetti, C.

AU - Gardeniers, H.J.G.E.

AU - Caputo, D.

AU - Nascetti, A.

AU - de Cesare, G.

N1 - Conference code: 4

PY - 2019

Y1 - 2019

N2 - This work presents a portable lab-on-chip system, based on thin film electronic devices and an all-glass microfluidic network, for the real-time monitoring of enzymatic chemiluminescent reactions. The microfluidic network is patterned, through wet etching, in a 1.1 mm-thick glass substrate that is subsequently bonded to a 0.5 mm-thick glass substrate. The electronic devices are amorphous silicon p-i-n photosensors, deposited on the outer side of the thinner glass substrate. The photosensors, the microfluidic network and the electronic boards reading out the photodiodes’ current are enclosed in a small metallic box (10×8×15×cm 3 ) in order to ensure shielding from electromagnetic interferences. Preliminary tests have been performed immobilizing horseradish peroxidase on the inner wall of the microchannel as model enzyme for detecting hydrogen peroxide. Limits of detection and quantification equal to 18 and 60 μM, respectively, have been found. These values are comparable to the best performances reported in literature for chemiluminescent-based optofluidic sensors.

AB - This work presents a portable lab-on-chip system, based on thin film electronic devices and an all-glass microfluidic network, for the real-time monitoring of enzymatic chemiluminescent reactions. The microfluidic network is patterned, through wet etching, in a 1.1 mm-thick glass substrate that is subsequently bonded to a 0.5 mm-thick glass substrate. The electronic devices are amorphous silicon p-i-n photosensors, deposited on the outer side of the thinner glass substrate. The photosensors, the microfluidic network and the electronic boards reading out the photodiodes’ current are enclosed in a small metallic box (10×8×15×cm 3 ) in order to ensure shielding from electromagnetic interferences. Preliminary tests have been performed immobilizing horseradish peroxidase on the inner wall of the microchannel as model enzyme for detecting hydrogen peroxide. Limits of detection and quantification equal to 18 and 60 μM, respectively, have been found. These values are comparable to the best performances reported in literature for chemiluminescent-based optofluidic sensors.

KW - Amorphous silicon

KW - Anodic bonding

KW - Enzymatic reactions

KW - Horseradish peroxidase

KW - Microfluidics

KW - Photosensors

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U2 - 10.1007/978-3-030-04324-7_25

DO - 10.1007/978-3-030-04324-7_25

M3 - Conference contribution

AN - SCOPUS:85061083410

SN - 9783030043230

T3 - Lecture Notes in Electrical Engineering

SP - 189

EP - 194

BT - Sensors - Proceedings of the 4th National Conference on Sensors, 2018

A2 - Baldini, Francesco

A2 - Siciliano, Pietro

A2 - Rossi, Marco

A2 - Scalise, Lorenzo

A2 - Di Natale, Corrado

A2 - Ferrari, Vittorio

A2 - Militello, Valeria

A2 - Miolo, Giorgia

A2 - Andò, Bruno

A2 - Marletta, Vincenzo

A2 - Marrazza, Giovanna

PB - Springer

T2 - 4th National Conference on Sensors, 2018

Y2 - 21 February 2018 through 23 February 2018

ER -

Costantini F, Tiggelaar RM, Salvio R, Nardecchia M, Schlautmann S, Manetti C et al. Portable optoelectronic system for monitoring enzymatic chemiluminescent reaction. In Baldini F, Siciliano P, Rossi M, Scalise L, Di Natale C, Ferrari V, Militello V, Miolo G, Andò B, Marletta V, Marrazza G, editors, Sensors - Proceedings of the 4th National Conference on Sensors, 2018. Springer. 2019. p. 189-194. (Lecture Notes in Electrical Engineering). doi: 10.1007/978-3-030-04324-7_25

Portable optoelectronic system for monitoring enzymatic chemiluminescent reaction

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