Portable optoelectronic system for monitoring enzymatic chemiluminescent reaction

F. Costantini, R. M. Tiggelaar, R. Salvio, M. Nardecchia, S. Schlautmann, C. Manetti, H. J.G.E. Gardeniers, D. Caputo*, A. Nascetti, G. de Cesare

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

This work presents a portable lab-on-chip system, based on thin film electronic devices and an all-glass microfluidic network, for the real-time monitoring of enzymatic chemiluminescent reactions. The microfluidic network is patterned, through wet etching, in a 1.1 mm-thick glass substrate that is subsequently bonded to a 0.5 mm-thick glass substrate. The electronic devices are amorphous silicon p-i-n photosensors, deposited on the outer side of the thinner glass substrate. The photosensors, the microfluidic network and the electronic boards reading out the photodiodes’ current are enclosed in a small metallic box (10×8×15×cm 3 ) in order to ensure shielding from electromagnetic interferences. Preliminary tests have been performed immobilizing horseradish peroxidase on the inner wall of the microchannel as model enzyme for detecting hydrogen peroxide. Limits of detection and quantification equal to 18 and 60 μM, respectively, have been found. These values are comparable to the best performances reported in literature for chemiluminescent-based optofluidic sensors.

Original languageEnglish
Title of host publicationSensors - Proceedings of the 4th National Conference on Sensors, 2018
EditorsFrancesco Baldini, Pietro Siciliano, Marco Rossi, Lorenzo Scalise, Corrado Di Natale, Vittorio Ferrari, Valeria Militello, Giorgia Miolo, Bruno Andò, Vincenzo Marletta, Giovanna Marrazza
PublisherSpringer Verlag
Pages189-194
Number of pages6
ISBN (Print)9783030043230
DOIs
Publication statusPublished - 2019
Event4th National Conference on Sensors, 2018 - Catania, Italy
Duration: 21 Feb 201823 Feb 2018
Conference number: 4

Publication series

NameLecture Notes in Electrical Engineering
Volume539
ISSN (Print)1876-1100
ISSN (Electronic)1876-1119

Conference

Conference4th National Conference on Sensors, 2018
CountryItaly
CityCatania
Period21/02/1823/02/18

Keywords

  • Amorphous silicon
  • Anodic bonding
  • Enzymatic reactions
  • Horseradish peroxidase
  • Microfluidics
  • Photosensors

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