Preclinical detection of liver fibrosis using dual-modality photoacoustic/ultrasound system

P.J. van den Berg, Ruchi Bansal, Khalid Daoudi, Wiendelt Steenbergen, Jai Prakash

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Abstract

Liver fibrosis is a major cause for increasing mortality worldwide. Preclinical research using animal models is required for the discovery of new anti-fibrotic therapies, but currently relies on endpoint liver histology. In this study, we investigated a cost-effective and portable photoacoustic/ultrasound (PA/US) imaging system as a potential non-invasive alternative. Fibrosis was induced in mice using CCl4 followed by liver imaging and histological analysis. Imaging showed significantly increased PA features with higher frequency signals in fibrotic livers versus healthy livers. This corresponds to more heterogeneous liver structure resulting from collagen deposition and angiogenesis. Importantly, PA response and its frequency were highly correlated with histological parameters. These results demonstrate the preclinical feasibility of the PA imaging approach and applicability of dual PA/US system.
Original languageEnglish
Pages (from-to)5081-5091
JournalBiomedical optics express
Volume7
Issue number12
DOIs
Publication statusPublished - 2016

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fibrosis
liver
Liver Cirrhosis
Liver
angiogenesis
animal models
histology
mortality
collagens
Ultrasonography
Histology
Fibrosis
Collagen
Animal Models
mice
therapy
Costs and Cost Analysis
Mortality
costs
Research

Keywords

  • METIS-321090
  • IR-103419

Cite this

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abstract = "Liver fibrosis is a major cause for increasing mortality worldwide. Preclinical research using animal models is required for the discovery of new anti-fibrotic therapies, but currently relies on endpoint liver histology. In this study, we investigated a cost-effective and portable photoacoustic/ultrasound (PA/US) imaging system as a potential non-invasive alternative. Fibrosis was induced in mice using CCl4 followed by liver imaging and histological analysis. Imaging showed significantly increased PA features with higher frequency signals in fibrotic livers versus healthy livers. This corresponds to more heterogeneous liver structure resulting from collagen deposition and angiogenesis. Importantly, PA response and its frequency were highly correlated with histological parameters. These results demonstrate the preclinical feasibility of the PA imaging approach and applicability of dual PA/US system.",
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Preclinical detection of liver fibrosis using dual-modality photoacoustic/ultrasound system. / van den Berg, P.J.; Bansal, Ruchi; Daoudi, Khalid; Steenbergen, Wiendelt; Prakash, Jai.

In: Biomedical optics express, Vol. 7, No. 12, 2016, p. 5081-5091.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Preclinical detection of liver fibrosis using dual-modality photoacoustic/ultrasound system

AU - van den Berg, P.J.

AU - Bansal, Ruchi

AU - Daoudi, Khalid

AU - Steenbergen, Wiendelt

AU - Prakash, Jai

N1 - Open access

PY - 2016

Y1 - 2016

N2 - Liver fibrosis is a major cause for increasing mortality worldwide. Preclinical research using animal models is required for the discovery of new anti-fibrotic therapies, but currently relies on endpoint liver histology. In this study, we investigated a cost-effective and portable photoacoustic/ultrasound (PA/US) imaging system as a potential non-invasive alternative. Fibrosis was induced in mice using CCl4 followed by liver imaging and histological analysis. Imaging showed significantly increased PA features with higher frequency signals in fibrotic livers versus healthy livers. This corresponds to more heterogeneous liver structure resulting from collagen deposition and angiogenesis. Importantly, PA response and its frequency were highly correlated with histological parameters. These results demonstrate the preclinical feasibility of the PA imaging approach and applicability of dual PA/US system.

AB - Liver fibrosis is a major cause for increasing mortality worldwide. Preclinical research using animal models is required for the discovery of new anti-fibrotic therapies, but currently relies on endpoint liver histology. In this study, we investigated a cost-effective and portable photoacoustic/ultrasound (PA/US) imaging system as a potential non-invasive alternative. Fibrosis was induced in mice using CCl4 followed by liver imaging and histological analysis. Imaging showed significantly increased PA features with higher frequency signals in fibrotic livers versus healthy livers. This corresponds to more heterogeneous liver structure resulting from collagen deposition and angiogenesis. Importantly, PA response and its frequency were highly correlated with histological parameters. These results demonstrate the preclinical feasibility of the PA imaging approach and applicability of dual PA/US system.

KW - METIS-321090

KW - IR-103419

U2 - 10.1364/BOE.7.005081

DO - 10.1364/BOE.7.005081

M3 - Article

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EP - 5091

JO - Biomedical optics express

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SN - 2156-7085

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