Preparation and evaluation of molucularly-defined collagen-elastin-glycosaminoglycan scaffolds for tissue engineering

W.F. Daamen, H.Th.B van Moerkerk, H. van Moerkerk, T. Hafmans, L. Buttafoco, Andreas A. Poot, J.H. Veerkamp, T.H. van Kuppevelt

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Abstract

Extracellular matrix components are valuable building blocks for the preparation of biomaterials involved in tissue engineering, especially if their biological, chemical and physical characteristics can be controlled. In this study, isolated type I collagen fibrils, elastin fibres and chondroitin sulphate (CS) were used for the preparation of molecularly-defined collagen-elastin-glycosaminoglycan scaffolds. A total of 12 different scaffolds were prepared with four different ratios of collagen and elastin (1:9, 1:1, 9:1 and 1:0), with and without chemical crosslinking, and with and without CS. Collagen was essential to fabricate coherent, porous scaffolds. Electron microscopy showed that collagen and elastin physically interacted with each other and that elastin fibres were enveloped by collagen. By carbodiimide-crosslinking, amine groups were coupled to carboxylic groups and CS could be incorporated. More CS could be bound to collagen scaffolds (10%) than to collagen-elastin scaffolds (2.4-8.5% depending on the ratio). The attachment of CS increased the water-binding capacity to up to 65%. Scaffolds with a higher collagen content had a higher tensile strength whereas addition of elastin increased elasticity. Scaffolds were cytocompatible as was established using human myoblast and fibroblast culture systems. It is concluded that molecularly-defined composite scaffolds can be composed from individual, purified, extracellular matrix components. Data are important in the design and application of tailor-made biomaterials for tissue engineering.
Original languageUndefined
Pages (from-to)4001-4009
JournalBiomaterials
Volume24
Issue number22
DOIs
Publication statusPublished - 2003

Keywords

  • IR-67201
  • Glycosaminoglycans
  • Collagen
  • Biomaterial
  • Tissue Engineering
  • METIS-217030
  • Elastin

Cite this

Daamen, W. F., van Moerkerk, H. T. B., van Moerkerk, H., Hafmans, T., Buttafoco, L., Poot, A. A., ... van Kuppevelt, T. H. (2003). Preparation and evaluation of molucularly-defined collagen-elastin-glycosaminoglycan scaffolds for tissue engineering. Biomaterials, 24(22), 4001-4009. https://doi.org/10.1016/S0142-9612(03)00273-4
Daamen, W.F. ; van Moerkerk, H.Th.B ; van Moerkerk, H. ; Hafmans, T. ; Buttafoco, L. ; Poot, Andreas A. ; Veerkamp, J.H. ; van Kuppevelt, T.H. / Preparation and evaluation of molucularly-defined collagen-elastin-glycosaminoglycan scaffolds for tissue engineering. In: Biomaterials. 2003 ; Vol. 24, No. 22. pp. 4001-4009.
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abstract = "Extracellular matrix components are valuable building blocks for the preparation of biomaterials involved in tissue engineering, especially if their biological, chemical and physical characteristics can be controlled. In this study, isolated type I collagen fibrils, elastin fibres and chondroitin sulphate (CS) were used for the preparation of molecularly-defined collagen-elastin-glycosaminoglycan scaffolds. A total of 12 different scaffolds were prepared with four different ratios of collagen and elastin (1:9, 1:1, 9:1 and 1:0), with and without chemical crosslinking, and with and without CS. Collagen was essential to fabricate coherent, porous scaffolds. Electron microscopy showed that collagen and elastin physically interacted with each other and that elastin fibres were enveloped by collagen. By carbodiimide-crosslinking, amine groups were coupled to carboxylic groups and CS could be incorporated. More CS could be bound to collagen scaffolds (10{\%}) than to collagen-elastin scaffolds (2.4-8.5{\%} depending on the ratio). The attachment of CS increased the water-binding capacity to up to 65{\%}. Scaffolds with a higher collagen content had a higher tensile strength whereas addition of elastin increased elasticity. Scaffolds were cytocompatible as was established using human myoblast and fibroblast culture systems. It is concluded that molecularly-defined composite scaffolds can be composed from individual, purified, extracellular matrix components. Data are important in the design and application of tailor-made biomaterials for tissue engineering.",
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author = "W.F. Daamen and {van Moerkerk}, H.Th.B and {van Moerkerk}, H. and T. Hafmans and L. Buttafoco and Poot, {Andreas A.} and J.H. Veerkamp and {van Kuppevelt}, T.H.",
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Daamen, WF, van Moerkerk, HTB, van Moerkerk, H, Hafmans, T, Buttafoco, L, Poot, AA, Veerkamp, JH & van Kuppevelt, TH 2003, 'Preparation and evaluation of molucularly-defined collagen-elastin-glycosaminoglycan scaffolds for tissue engineering' Biomaterials, vol. 24, no. 22, pp. 4001-4009. https://doi.org/10.1016/S0142-9612(03)00273-4

Preparation and evaluation of molucularly-defined collagen-elastin-glycosaminoglycan scaffolds for tissue engineering. / Daamen, W.F.; van Moerkerk, H.Th.B; van Moerkerk, H.; Hafmans, T.; Buttafoco, L.; Poot, Andreas A.; Veerkamp, J.H.; van Kuppevelt, T.H.

In: Biomaterials, Vol. 24, No. 22, 2003, p. 4001-4009.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Preparation and evaluation of molucularly-defined collagen-elastin-glycosaminoglycan scaffolds for tissue engineering

AU - Daamen, W.F.

AU - van Moerkerk, H.Th.B

AU - van Moerkerk, H.

AU - Hafmans, T.

AU - Buttafoco, L.

AU - Poot, Andreas A.

AU - Veerkamp, J.H.

AU - van Kuppevelt, T.H.

PY - 2003

Y1 - 2003

N2 - Extracellular matrix components are valuable building blocks for the preparation of biomaterials involved in tissue engineering, especially if their biological, chemical and physical characteristics can be controlled. In this study, isolated type I collagen fibrils, elastin fibres and chondroitin sulphate (CS) were used for the preparation of molecularly-defined collagen-elastin-glycosaminoglycan scaffolds. A total of 12 different scaffolds were prepared with four different ratios of collagen and elastin (1:9, 1:1, 9:1 and 1:0), with and without chemical crosslinking, and with and without CS. Collagen was essential to fabricate coherent, porous scaffolds. Electron microscopy showed that collagen and elastin physically interacted with each other and that elastin fibres were enveloped by collagen. By carbodiimide-crosslinking, amine groups were coupled to carboxylic groups and CS could be incorporated. More CS could be bound to collagen scaffolds (10%) than to collagen-elastin scaffolds (2.4-8.5% depending on the ratio). The attachment of CS increased the water-binding capacity to up to 65%. Scaffolds with a higher collagen content had a higher tensile strength whereas addition of elastin increased elasticity. Scaffolds were cytocompatible as was established using human myoblast and fibroblast culture systems. It is concluded that molecularly-defined composite scaffolds can be composed from individual, purified, extracellular matrix components. Data are important in the design and application of tailor-made biomaterials for tissue engineering.

AB - Extracellular matrix components are valuable building blocks for the preparation of biomaterials involved in tissue engineering, especially if their biological, chemical and physical characteristics can be controlled. In this study, isolated type I collagen fibrils, elastin fibres and chondroitin sulphate (CS) were used for the preparation of molecularly-defined collagen-elastin-glycosaminoglycan scaffolds. A total of 12 different scaffolds were prepared with four different ratios of collagen and elastin (1:9, 1:1, 9:1 and 1:0), with and without chemical crosslinking, and with and without CS. Collagen was essential to fabricate coherent, porous scaffolds. Electron microscopy showed that collagen and elastin physically interacted with each other and that elastin fibres were enveloped by collagen. By carbodiimide-crosslinking, amine groups were coupled to carboxylic groups and CS could be incorporated. More CS could be bound to collagen scaffolds (10%) than to collagen-elastin scaffolds (2.4-8.5% depending on the ratio). The attachment of CS increased the water-binding capacity to up to 65%. Scaffolds with a higher collagen content had a higher tensile strength whereas addition of elastin increased elasticity. Scaffolds were cytocompatible as was established using human myoblast and fibroblast culture systems. It is concluded that molecularly-defined composite scaffolds can be composed from individual, purified, extracellular matrix components. Data are important in the design and application of tailor-made biomaterials for tissue engineering.

KW - IR-67201

KW - Glycosaminoglycans

KW - Collagen

KW - Biomaterial

KW - Tissue Engineering

KW - METIS-217030

KW - Elastin

U2 - 10.1016/S0142-9612(03)00273-4

DO - 10.1016/S0142-9612(03)00273-4

M3 - Article

VL - 24

SP - 4001

EP - 4009

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 22

ER -