Preparation of Biomolecule Microstructures and Microarrays by Thiol–ene Photoimmobilization

Dirk Weinrich, Maja Köhn, Pascal Jonkheijm, Ulrika Westerlind, Leif Dehmelt, Hans Engelkamp, Peter C.M. Christianen, Jürgen Kuhlmann, Jan C. Maan, Dirk Nüsse, Hendrik Schröder, Ron Wacker, Edgar Voges , Rolf Breinbauer, Horst Kunz, Christof M. Niemeyer, Herbert Waldmann

Research output: Contribution to journalArticleAcademic

46 Citations (Scopus)

Abstract

A mild, fast and flexible method for photoimmobilization of biomolecules based on the light-initiated thiol–ene reaction has been developed. After investigation and optimization of various surface materials, surface chemistries and reaction parameters, microstructures and microarrays of biotin, oligonucleotides, peptides, and MUC1 tandem repeat glycopeptides were prepared with this photoimmobilization method. Furthermore, MUC1 tandem repeat glycopeptide microarrays were successfully used to probe antibodies in mouse serum obtained from vaccinated mice. Dimensions of biomolecule microstructures were shown to be freely controllable through photolithographic techniques, and features down to 5 μm in size covering an area of up to 75×25 mm were created. Use of a confocal laser microscope with a UV laser as UV-light source enabled further reduction of biotin feature size opening access to nanostructured biochips.
Original languageUndefined
Pages (from-to)235-247
JournalChemBioChem
Volume11
Issue number2
DOIs
Publication statusPublished - 2010

Keywords

  • photochemistry
  • IR-72760
  • thiol–ene reaction
  • Biochips
  • Microarrays
  • immobilization

Cite this