TY - JOUR
T1 - Presence and strength of binding of IgM, IgG and IgA antibodies against SARS-CoV-2 during CoViD-19 infection
AU - Schasfoort, Richard B.M.
AU - van Weperen, Jos
AU - van Amsterdam, Margot
AU - Parisot, Judicaël
AU - Hendriks, Jan
AU - Koerselman, Michelle
AU - Karperien, Marcel
AU - Mentink, Anouk
AU - Bennink, Martin
AU - Krabbe, Hans
AU - Terstappen, Leon W.M.M.
AU - Mulder, A.H. Leontine
N1 - Funding Information:
The study was performed in accordance with the guidelines for sharing of patient data of observational scientific research in emergency situations as issued by the Commission on Codes of Conduct of the Foundation Federation of Dutch Medical Scientific Societies (https://www.federa.org/federa-english). This project has received funding from the ATTRACT project funded by the EC under Grant Agreement 777222. The Dutch Research Council (NWO), (Kirsten Ampt) is acknowledged for support of sensors and reagents.
Funding Information:
The study was performed in accordance with the guidelines for sharing of patient data of observational scientific research in emergency situations as issued by the Commission on Codes of Conduct of the Foundation Federation of Dutch Medical Scientific Societies ( https://www.federa.org/federa-english ). This project has received funding from the ATTRACT project funded by the EC under Grant Agreement 777222 . The Dutch Research Council (NWO), (Kirsten Ampt) is acknowledged for support of sensors and reagents.
Publisher Copyright:
© 2021 The Author(s)
PY - 2021/7/1
Y1 - 2021/7/1
N2 - Surface Plasmon Resonance imaging (SPRi) was used to determine the presence and strength of binding of IgG, IgM and IgA against the Receptor Binding Domain (RBD) of SARS-CoV-2 in sera of 119 CoViD-19 patients. The SPRi assay measures the antibody isotype levels and the strength of binding to the RBD of ultimate 384 patient samples in one run. It turns out that during the course of the disease, the IgG levels and strength of binding increased while generally the IgM and IgA levels go down. Recovered patients all show high strength of binding of the IgG type to the RBD protein. The anti-RBD immunoglobulins SPRi assay provides additional insights in the immune status of patients recovering from CoViD-19 and this new method can furthermore be applied for the assessment of the quality of the immune reaction of healthy individuals to SARS-CoV-2 in vaccination programs.
AB - Surface Plasmon Resonance imaging (SPRi) was used to determine the presence and strength of binding of IgG, IgM and IgA against the Receptor Binding Domain (RBD) of SARS-CoV-2 in sera of 119 CoViD-19 patients. The SPRi assay measures the antibody isotype levels and the strength of binding to the RBD of ultimate 384 patient samples in one run. It turns out that during the course of the disease, the IgG levels and strength of binding increased while generally the IgM and IgA levels go down. Recovered patients all show high strength of binding of the IgG type to the RBD protein. The anti-RBD immunoglobulins SPRi assay provides additional insights in the immune status of patients recovering from CoViD-19 and this new method can furthermore be applied for the assessment of the quality of the immune reaction of healthy individuals to SARS-CoV-2 in vaccination programs.
KW - UT-Hybrid-D
KW - Biosensor
KW - off-rate
KW - SPR
KW - Surface plasmon resonance imaging
KW - Affinity
UR - http://www.scopus.com/inward/record.url?scp=85104352916&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2021.113165
DO - 10.1016/j.bios.2021.113165
M3 - Article
C2 - 33799060
AN - SCOPUS:85104352916
SN - 0956-5663
VL - 183
JO - Biosensors & bioelectronics
JF - Biosensors & bioelectronics
M1 - 113165
ER -
