Pressure-Driven Perfusion System to Control, Multiplex and Recirculate Cell Culture Medium for Organs-on-Chips

Mees N.S. de Graaf, Aisen Vivas, Andries D. van der Meer, Christine L. Mummery, Valeria V. Orlova*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)
57 Downloads (Pure)

Abstract

Organ-on-chip (OoC) devices are increasingly used to mimic the tissue microenvironment of cells in intact organs. This includes microchannels to mimic, for example, fluidic flow through blood vessels. Present methods for controlling microfluidic flow in these systems rely on gravity, rocker systems or external pressure pumps. For many purposes, pressure pumps give the most consistent flow profiles, but they are not well-suited for high throughput as might be required for testing drug responses. Here, we describe a method which allows for multiplexing of microfluidic channels in OoC devices plus the accompanying custom software necessary to run the system. Moreover, we show the approach is also suitable for recirculation of culture medium, an essential cost consideration when expensive culture reagents are used and are not “spent” through uptake by the cells during transient unidirectional flow.

Original languageEnglish
Article number1359
JournalMicromachines
Volume13
Issue number8
DOIs
Publication statusPublished - 20 Aug 2022

Keywords

  • Fluidic circuit board (FCB)
  • Multiplexing
  • Organ-on-a-chip (OoC)
  • Perfusion
  • Recirculation
  • Vessels-on-chip (VoC)
  • UT-Gold-D

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