Primary chondrocytes enhance cartilage tissue formation upon co-culture with expanded chondrocytes, dermal fibroblasts, 3T3 feeder cells and embryonic stem cells

J.A.A. Hendriks, Razvan L. Miclea, Roka Schotel, Ewart de Bruijn, Lorenzo Moroni, Hermanus Bernardus Johannes Karperien, J.U. Riesle, Clemens van Blitterswijk

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

Co-culture models have been increasingly used in tissue engineering applications to understand cell–cell interactions and consequently improve regenerative medicine strategies. Aiming at further elucidating cartilage tissue formation, we co-cultured bovine primary chondrocytes (BPCs) with human expanded chondrocytes (HECs), human dermal fibroblasts (HDFs), mouse embryonic stem cells (MESCs), or mouse-3T3 feeder cells (M3T3s) in micromasses. BPCs were either co-cultured (1:5 ratio) with all cell types allowing direct cell–cell contacts or as separate micromasses in the same well with HECs. In co-culture groups with direct cell–cell contacts cartilaginous tissue was formed in all experimental groups. In situ hybridization showed that only 16–27% of the cells expressed type II collagen mRNA. Corresponding with the fact that micromasses consisted for approximately 20% only of BPCs, the amount of GAG was similar between 100% BPC micromass and the co-culture groups with HECs and HDFs. Therefore, co-culture micromasses support cartilage tissue formation predominantly originating from primary chondrocytes in direct contact with a variety of cell types. These findings potentially could be applied to optimize cell-therapy treatments for cartilage regeneration.
Original languageEnglish
Pages (from-to)5080-5088
JournalSoft matter
Volume6
Issue number20
DOIs
Publication statusPublished - 2010

Keywords

  • IR-80628
  • METIS-274256

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