Prism-based excitation wavelength selection for multicolor fluorescence coincidence measurements

R. Kassies, Aufrid T.M. Lenferink, Gezina M.J. Segers-Nolten, Cornelis Otto

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)


We have designed and constructed a prism setup for multiple-color confocal fluorescence experiments. The prism setup permits easy selection of any color or any combination of colors from a multicolor light source, such as a mixed-gas argon-krypton-ion laser. The selected colors emerging from the prism setup are, by design, optimally overlapped in the focus of a high-numerical-aperture objective, such as that commonly used in single-molecule fluorescence experiments. The various excitation powers can be easily adjusted in this setup. We will exemplify the potential of this setup in single-molecule fluorescence emission spectroscopy
Original languageUndefined
Pages (from-to)893-897
Number of pages5
JournalApplied Optics
Issue number6
Publication statusPublished - 2005


  • IR-96425
  • METIS-223815

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