Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 83A-83A |
| Number of pages | 1 |
| Journal | Biophysical journal |
| Volume | 106 |
| Issue number | 2, Suppl. 1 |
| DOIs | |
| Publication status | Published - 16 Feb 2014 |
| Event | 58th Annual Biophysical Society Meeting 2014 - San Francisco, United States Duration: 15 Feb 2014 → 19 Feb 2014 Conference number: 58 |
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Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format. / Stimberg, Verena C.; Bomer, Johan G.; de Boer, Hans L.; van den Berg, Albert; le Gac, Severine.
In: Biophysical journal, Vol. 106, No. 2, Suppl. 1, 16.02.2014, p. 83A-83A.Research output: Contribution to journal › Article › Academic
TY - JOUR
T1 - Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format
AU - Stimberg, Verena C.
AU - Bomer, Johan G.
AU - de Boer, Hans L.
AU - van den Berg, Albert
AU - le Gac, Severine
PY - 2014/2/16
Y1 - 2014/2/16
N2 - Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.
AB - Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.
U2 - 10.1016/j.bpj.2013.11.536
DO - 10.1016/j.bpj.2013.11.536
M3 - Article
VL - 106
SP - 83A-83A
JO - Biophysical journal
JF - Biophysical journal
SN - 0006-3495
IS - 2, Suppl. 1
ER -