Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format

Verena C. Stimberg, Johan G. Bomer, Hans L. de Boer, Albert van den Berg, Severine le Gac

    Research output: Contribution to journal › Article › Academic

    Abstract

    Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.
    Original languageEnglish
    Pages (from-to)83A-83A
    Number of pages1
    JournalBiophysical journal
    Volume106
    Issue number2, Suppl. 1
    DOIs
    Publication statusPublished - 16 Feb 2014
    Event58th Annual Biophysical Society Meeting 2014 - San Francisco, United States
    Duration: 15 Feb 2014 → 19 Feb 2014
    Conference number: 58

    Fingerprint

    Microfluidics
    Ion Channels
    Membrane Proteins
    Membrane Fluidity
    Membranes
    Lipid Bilayers
    Membrane Lipids
    Phospholipids
    Cell Membrane

    Cite this

    Stimberg, Verena C. ; Bomer, Johan G. ; de Boer, Hans L. ; van den Berg, Albert ; le Gac, Severine. / Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format. In: Biophysical journal. 2014 ; Vol. 106, No. 2, Suppl. 1. pp. 83A-83A.
    @article{da72b5ceeb9b473f9a779db366adc386,
    title = "Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format",
    abstract = "Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.",
    author = "Stimberg, {Verena C.} and Bomer, {Johan G.} and {de Boer}, {Hans L.} and {van den Berg}, Albert and {le Gac}, Severine",
    year = "2014",
    month = "2",
    day = "16",
    doi = "10.1016/j.bpj.2013.11.536",
    language = "English",
    volume = "106",
    pages = "83A--83A",
    journal = "Biophysical journal",
    issn = "0006-3495",
    publisher = "Biophysical Society",
    number = "2, Suppl. 1",

    }

    Stimberg, VC, Bomer, JG, de Boer, HL, van den Berg, A & le Gac, S 2014, 'Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format', Biophysical journal, vol. 106, no. 2, Suppl. 1, pp. 83A-83A. https://doi.org/10.1016/j.bpj.2013.11.536

    Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format. / Stimberg, Verena C.; Bomer, Johan G.; de Boer, Hans L.; van den Berg, Albert; le Gac, Severine.

    In: Biophysical journal, Vol. 106, No. 2, Suppl. 1, 16.02.2014, p. 83A-83A.

    Research output: Contribution to journal › Article › Academic

    TY - JOUR

    T1 - Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format

    AU - Stimberg, Verena C.

    AU - Bomer, Johan G.

    AU - de Boer, Hans L.

    AU - van den Berg, Albert

    AU - le Gac, Severine

    PY - 2014/2/16

    Y1 - 2014/2/16

    N2 - Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.

    AB - Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.

    U2 - 10.1016/j.bpj.2013.11.536

    DO - 10.1016/j.bpj.2013.11.536

    M3 - Article

    VL - 106

    SP - 83A-83A

    JO - Biophysical journal

    JF - Biophysical journal

    SN - 0006-3495

    IS - 2, Suppl. 1

    ER -

    Stimberg VC, Bomer JG, de Boer HL, van den Berg A, le Gac S. Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format. Biophysical journal. 2014 Feb 16;106(2, Suppl. 1):83A-83A. https://doi.org/10.1016/j.bpj.2013.11.536

    Access to Document