Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format

    Research output: Contribution to journalArticleAcademic

    Abstract

    Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.
    Original languageEnglish
    Pages (from-to)83A-83A
    Number of pages1
    JournalBiophysical journal
    Volume106
    Issue number2, Suppl. 1
    DOIs
    Publication statusPublished - 16 Feb 2014
    Event58th Annual Biophysical Society Meeting 2014 - San Francisco, United States
    Duration: 15 Feb 201419 Feb 2014
    Conference number: 58

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    Microfluidics
    Ion Channels
    Membrane Proteins
    Membrane Fluidity
    Membranes
    Lipid Bilayers
    Membrane Lipids
    Phospholipids
    Cell Membrane

    Cite this

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    abstract = "Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.",
    author = "Stimberg, {Verena C.} and Bomer, {Johan G.} and {de Boer}, {Hans L.} and {van den Berg}, Albert and {le Gac}, Severine",
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    AU - Stimberg, Verena C.

    AU - Bomer, Johan G.

    AU - de Boer, Hans L.

    AU - van den Berg, Albert

    AU - le Gac, Severine

    PY - 2014/2/16

    Y1 - 2014/2/16

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    AB - Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.

    U2 - 10.1016/j.bpj.2013.11.536

    DO - 10.1016/j.bpj.2013.11.536

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    JO - Biophysical journal

    JF - Biophysical journal

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