Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format

Verena C. Stimberg; Johan G. Bomer; Hans L. de Boer; Albert van den Berg; Severine le Gac

doi:10.1016/j.bpj.2013.11.536

Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format

Verena C. Stimberg, Johan G. Bomer, Hans L. de Boer, Albert van den Berg, Severine le Gac

Research output: Contribution to journal › Article › Academic

Abstract

Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.

Original language	English
Pages (from-to)	83A-83A
Number of pages	1
Journal	Biophysical journal
Volume	106
Issue number	2, Suppl. 1
DOIs	https://doi.org/10.1016/j.bpj.2013.11.536
Publication status	Published - 16 Feb 2014
Event	58th Annual Biophysical Society Meeting 2014 - San Francisco, United States Duration: 15 Feb 2014 → 19 Feb 2014 Conference number: 58

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title = "Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format",

abstract = "Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.",

author = "Stimberg, {Verena C.} and Bomer, {Johan G.} and {de Boer}, {Hans L.} and {van den Berg}, Albert and {le Gac}, Severine",

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T1 - Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format

AU - Stimberg, Verena C.

AU - Bomer, Johan G.

AU - de Boer, Hans L.

AU - van den Berg, Albert

AU - le Gac, Severine

N1 - Conference code: 58

PY - 2014/2/16

Y1 - 2014/2/16

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AB - Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.

U2 - 10.1016/j.bpj.2013.11.536

DO - 10.1016/j.bpj.2013.11.536

M3 - Article

VL - 106

SP - 83A-83A

JO - Biophysical journal

JF - Biophysical journal

SN - 0006-3495

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T2 - 58th Annual Biophysical Society Meeting 2014

Y2 - 15 February 2014 through 19 February 2014

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