Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format

Research output: Contribution to journalArticleAcademic

Abstract

Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.
Original languageEnglish
Pages (from-to)83A-83A
Number of pages1
JournalBiophysical journal
Volume106
Issue number2, Suppl. 1
DOIs
Publication statusPublished - 16 Feb 2014
Event58th Annual Biophysical Society Meeting 2014 - San Francisco, United States
Duration: 15 Feb 201419 Feb 2014
Conference number: 58

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Microfluidics
Ion Channels
Membrane Proteins
Membrane Fluidity
Membranes
Lipid Bilayers
Membrane Lipids
Phospholipids
Cell Membrane

Cite this

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abstract = "Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.",
author = "Stimberg, {Verena C.} and Bomer, {Johan G.} and {de Boer}, {Hans L.} and {van den Berg}, Albert and {le Gac}, Severine",
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T1 - Probing simultaneously membrane dynamics and protein activity in suspended bilayers in a microfluidic format

AU - Stimberg, Verena C.

AU - Bomer, Johan G.

AU - de Boer, Hans L.

AU - van den Berg, Albert

AU - le Gac, Severine

PY - 2014/2/16

Y1 - 2014/2/16

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AB - Membrane dynamics affect the structure and function of ion channels, a point that deserves more attention while studying membrane proteins. One important factor in the local lipidic environment of the ion channels, is the membrane fluidity which is directly connected to the free diffusion and packing of the phospholipids. Typically, FRAP is utilized to investigate this parameter in simplified cell membrane models, and preferably supported bilayer lipid membranes (BLMs), which are more stable than their free-standing counterparts. However, supported membranes are less suitable for the incorporation of ion channels and electrophysiological monitoring of their activity than suspended ones.

U2 - 10.1016/j.bpj.2013.11.536

DO - 10.1016/j.bpj.2013.11.536

M3 - Article

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SN - 0006-3495

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