Progress and future of in vitro models to study translocation of nanoparticles

Hedwig M. Braakhuis, Samantha K. Kloet, Sanja Kezic, Frieke Kuper, Margriet V.D.Z. Park, Severine le Gac, Petra Krystek, Ruud J.B. Peters, Ivonne M.C.M. Rietjens, Hans Bouwmeester

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Abstract

The increasing use of nanoparticles in products likely results in increased exposure of both workers and consumers. Because of their small size, there are concerns that nanoparticles unintentionally cross the barriers of the human body. Several in vivo rodent studies show that, dependent on the exposure route, time, and concentration, and their characteristics, nanoparticles can cross the lung, gut, skin, and placental barrier. This review aims to evaluate the performance of in vitro models that mimic the barriers of the human body, with a focus on the lung, gut, skin, and placental barrier. For these barriers, in vitro models of varying complexity are available, ranging from single-cell-type monolayer to multi-cell (3D) models. Only a few studies are available that allow comparison of the in vitro translocation to in vivo data. This situation could change since the availability of analytical detection techniques is no longer a limiting factor for this comparison. We conclude that to further develop in vitro models to be used in risk assessment, the current strategy to improve the models to more closely mimic the human situation by using co-cultures of different cell types and microfluidic approaches to better control the tissue microenvironments are essential. At the current state of the art, the in vitro models do not yet allow prediction of absolute transfer rates but they do support the definition of relative transfer rates and can thus help to reduce animal testing by setting priorities for subsequent in vivo testing
Original languageUndefined
Pages (from-to)1469-1495
Number of pages27
JournalArchives of toxicology
Volume89
Issue number9
DOIs
Publication statusPublished - Sep 2015

Keywords

  • EWI-26767
  • METIS-315577
  • IR-99341

Cite this

Braakhuis, H. M., Kloet, S. K., Kezic, S., Kuper, F., Park, M. V. D. Z., le Gac, S., ... Bouwmeester, H. (2015). Progress and future of in vitro models to study translocation of nanoparticles. Archives of toxicology, 89(9), 1469-1495. https://doi.org/10.1007/s00204-015-1518-5
Braakhuis, Hedwig M. ; Kloet, Samantha K. ; Kezic, Sanja ; Kuper, Frieke ; Park, Margriet V.D.Z. ; le Gac, Severine ; Krystek, Petra ; Peters, Ruud J.B. ; Rietjens, Ivonne M.C.M. ; Bouwmeester, Hans. / Progress and future of in vitro models to study translocation of nanoparticles. In: Archives of toxicology. 2015 ; Vol. 89, No. 9. pp. 1469-1495.
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author = "Braakhuis, {Hedwig M.} and Kloet, {Samantha K.} and Sanja Kezic and Frieke Kuper and Park, {Margriet V.D.Z.} and {le Gac}, Severine and Petra Krystek and Peters, {Ruud J.B.} and Rietjens, {Ivonne M.C.M.} and Hans Bouwmeester",
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Braakhuis, HM, Kloet, SK, Kezic, S, Kuper, F, Park, MVDZ, le Gac, S, Krystek, P, Peters, RJB, Rietjens, IMCM & Bouwmeester, H 2015, 'Progress and future of in vitro models to study translocation of nanoparticles' Archives of toxicology, vol. 89, no. 9, pp. 1469-1495. https://doi.org/10.1007/s00204-015-1518-5

Progress and future of in vitro models to study translocation of nanoparticles. / Braakhuis, Hedwig M.; Kloet, Samantha K.; Kezic, Sanja; Kuper, Frieke; Park, Margriet V.D.Z.; le Gac, Severine; Krystek, Petra; Peters, Ruud J.B.; Rietjens, Ivonne M.C.M.; Bouwmeester, Hans.

In: Archives of toxicology, Vol. 89, No. 9, 09.2015, p. 1469-1495.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Progress and future of in vitro models to study translocation of nanoparticles

AU - Braakhuis, Hedwig M.

AU - Kloet, Samantha K.

AU - Kezic, Sanja

AU - Kuper, Frieke

AU - Park, Margriet V.D.Z.

AU - le Gac, Severine

AU - Krystek, Petra

AU - Peters, Ruud J.B.

AU - Rietjens, Ivonne M.C.M.

AU - Bouwmeester, Hans

N1 - Open access

PY - 2015/9

Y1 - 2015/9

N2 - The increasing use of nanoparticles in products likely results in increased exposure of both workers and consumers. Because of their small size, there are concerns that nanoparticles unintentionally cross the barriers of the human body. Several in vivo rodent studies show that, dependent on the exposure route, time, and concentration, and their characteristics, nanoparticles can cross the lung, gut, skin, and placental barrier. This review aims to evaluate the performance of in vitro models that mimic the barriers of the human body, with a focus on the lung, gut, skin, and placental barrier. For these barriers, in vitro models of varying complexity are available, ranging from single-cell-type monolayer to multi-cell (3D) models. Only a few studies are available that allow comparison of the in vitro translocation to in vivo data. This situation could change since the availability of analytical detection techniques is no longer a limiting factor for this comparison. We conclude that to further develop in vitro models to be used in risk assessment, the current strategy to improve the models to more closely mimic the human situation by using co-cultures of different cell types and microfluidic approaches to better control the tissue microenvironments are essential. At the current state of the art, the in vitro models do not yet allow prediction of absolute transfer rates but they do support the definition of relative transfer rates and can thus help to reduce animal testing by setting priorities for subsequent in vivo testing

AB - The increasing use of nanoparticles in products likely results in increased exposure of both workers and consumers. Because of their small size, there are concerns that nanoparticles unintentionally cross the barriers of the human body. Several in vivo rodent studies show that, dependent on the exposure route, time, and concentration, and their characteristics, nanoparticles can cross the lung, gut, skin, and placental barrier. This review aims to evaluate the performance of in vitro models that mimic the barriers of the human body, with a focus on the lung, gut, skin, and placental barrier. For these barriers, in vitro models of varying complexity are available, ranging from single-cell-type monolayer to multi-cell (3D) models. Only a few studies are available that allow comparison of the in vitro translocation to in vivo data. This situation could change since the availability of analytical detection techniques is no longer a limiting factor for this comparison. We conclude that to further develop in vitro models to be used in risk assessment, the current strategy to improve the models to more closely mimic the human situation by using co-cultures of different cell types and microfluidic approaches to better control the tissue microenvironments are essential. At the current state of the art, the in vitro models do not yet allow prediction of absolute transfer rates but they do support the definition of relative transfer rates and can thus help to reduce animal testing by setting priorities for subsequent in vivo testing

KW - EWI-26767

KW - METIS-315577

KW - IR-99341

U2 - 10.1007/s00204-015-1518-5

DO - 10.1007/s00204-015-1518-5

M3 - Article

VL - 89

SP - 1469

EP - 1495

JO - Archives of toxicology

JF - Archives of toxicology

SN - 0340-5761

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