Protein digest separations in silicon pillar arrays conformally coated with porous silica

Wim De Malsche; Selm De Bruyne; Jeff Op de Beeck; Sebastiaan Eeltink; Han Gardeniers; Gert Desmet

Protein digest separations in silicon pillar arrays conformally coated with porous silica

Wim De Malsche^*, Selm De Bruyne, Jeff Op de Beeck, Sebastiaan Eeltink, Han Gardeniers, Gert Desmet

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

The achievable separation performance of a 9 cm long pillar array channel containing 5 μm diameter silicon pillars (spacing 2.5 μm) cladded with a meso-porous silica layer with a thickness of 300 nm was investigated, interfacing the chip to a capillary LC instrument. The overall performance was evaluated by conducting gradient elution separations of digests of cytochrome c and bovine serum albumin. Peak capacities of up to 150 could be demonstrated.

Original language	English
Title of host publication	Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Publisher	The Chemical and Biological Microsystems Society
Pages	154-156
Number of pages	3
ISBN (Print)	9780979806452
Publication status	Published - 2012
Event	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: 28 Oct 2012 → 1 Nov 2012

Conference

Conference	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/Territory	Japan
City	Okinawa
Period	28/10/12 → 1/11/12

Keywords

Capillary LC
Chip chromatography
Pillar array column
Porous layer integration
Protein digest separation

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De Malsche, W., De Bruyne, S., Op de Beeck, J., Eeltink, S., Gardeniers, H., & Desmet, G. (2012). Protein digest separations in silicon pillar arrays conformally coated with porous silica. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 154-156). The Chemical and Biological Microsystems Society.

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title = "Protein digest separations in silicon pillar arrays conformally coated with porous silica",

abstract = "The achievable separation performance of a 9 cm long pillar array channel containing 5 μm diameter silicon pillars (spacing 2.5 μm) cladded with a meso-porous silica layer with a thickness of 300 nm was investigated, interfacing the chip to a capillary LC instrument. The overall performance was evaluated by conducting gradient elution separations of digests of cytochrome c and bovine serum albumin. Peak capacities of up to 150 could be demonstrated.",

keywords = "Capillary LC, Chip chromatography, Pillar array column, Porous layer integration, Protein digest separation",

author = "{De Malsche}, Wim and {De Bruyne}, Selm and {Op de Beeck}, Jeff and Sebastiaan Eeltink and Han Gardeniers and Gert Desmet",

year = "2012",

language = "English",

isbn = "9780979806452",

pages = "154--156",

booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",

publisher = "The Chemical and Biological Microsystems Society",

note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 ; Conference date: 28-10-2012 Through 01-11-2012",

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De Malsche, W, De Bruyne, S, Op de Beeck, J, Eeltink, S, Gardeniers, H & Desmet, G 2012, Protein digest separations in silicon pillar arrays conformally coated with porous silica. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. The Chemical and Biological Microsystems Society, pp. 154-156, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 28/10/12.

Protein digest separations in silicon pillar arrays conformally coated with porous silica. / De Malsche, Wim; De Bruyne, Selm; Op de Beeck, Jeff et al.
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. The Chemical and Biological Microsystems Society, 2012. p. 154-156.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Protein digest separations in silicon pillar arrays conformally coated with porous silica

AU - De Malsche, Wim

AU - De Bruyne, Selm

AU - Op de Beeck, Jeff

AU - Eeltink, Sebastiaan

AU - Gardeniers, Han

AU - Desmet, Gert

PY - 2012

Y1 - 2012

N2 - The achievable separation performance of a 9 cm long pillar array channel containing 5 μm diameter silicon pillars (spacing 2.5 μm) cladded with a meso-porous silica layer with a thickness of 300 nm was investigated, interfacing the chip to a capillary LC instrument. The overall performance was evaluated by conducting gradient elution separations of digests of cytochrome c and bovine serum albumin. Peak capacities of up to 150 could be demonstrated.

AB - The achievable separation performance of a 9 cm long pillar array channel containing 5 μm diameter silicon pillars (spacing 2.5 μm) cladded with a meso-porous silica layer with a thickness of 300 nm was investigated, interfacing the chip to a capillary LC instrument. The overall performance was evaluated by conducting gradient elution separations of digests of cytochrome c and bovine serum albumin. Peak capacities of up to 150 could be demonstrated.

KW - Capillary LC

KW - Chip chromatography

KW - Pillar array column

KW - Porous layer integration

KW - Protein digest separation

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M3 - Conference contribution

AN - SCOPUS:84901774185

SN - 9780979806452

SP - 154

EP - 156

BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

PB - The Chemical and Biological Microsystems Society

T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Y2 - 28 October 2012 through 1 November 2012

ER -

Protein digest separations in silicon pillar arrays conformally coated with porous silica

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