Protein-repellent functionalizable surfaces based on covalently bonded phospholipids with phosphorylcholine head

L. Ferez; T. Thami; L. Tauk; E. Akpalo; V. Flaud; A. Kocer; J.-M. Janot; P. Déjardin

doi:10.1021/bk-2012-1120.ch031

Protein-repellent functionalizable surfaces based on covalently bonded phospholipids with phosphorylcholine head

L. Ferez, T. Thami, L. Tauk, E. Akpalo, V. Flaud, A. Kocer, J.-M. Janot, P. Déjardin

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

Surface anchored poly(methylhydrosiloxane) (PMHS) films (≈ 10 nm – 1µm) were functionalized with 1,2-dilinoleoyl-sn-glycero-3-phosphorylcholine (18:2 Cis). The surface was characterized by X-ray photoelectron spectroscopy, contact angle measurements, atomic force microscopy, and scanning electron microscopy. The interface was very hydrophilic and repellent toward avidin, bovine serum albumin, bovine fibrinogen, lysozyme, cytochrome C and α-chymotrypsin at pH 7.4 as observed by quantitative normal scanning confocal fluorescence. The surface is also repellent to liposomes (400 nm diameter) of L-α-phosphatidylcholine (Soy-20%). Further possibilities of functionalization on the surface remain available owing to the formation of interfacial SiOH groups.

Original language	English
Title of host publication	Proteins at Interfaces III State of the Art
Chapter	31
Pages	677-692
ISBN (Electronic)	9780841227972
DOIs	https://doi.org/10.1021/bk-2012-1120.ch031
Publication status	Published - 2012
Externally published	Yes

Publication series

Name

Name	ACS Symposium Series
Volume	1120

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10.1021/bk-2012-1120.ch031

Cite this

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title = "Protein-repellent functionalizable surfaces based on covalently bonded phospholipids with phosphorylcholine head",

abstract = "Surface anchored poly(methylhydrosiloxane) (PMHS) films (≈ 10 nm – 1µm) were functionalized with 1,2-dilinoleoyl-sn-glycero-3-phosphorylcholine (18:2 Cis). The surface was characterized by X-ray photoelectron spectroscopy, contact angle measurements, atomic force microscopy, and scanning electron microscopy. The interface was very hydrophilic and repellent toward avidin, bovine serum albumin, bovine fibrinogen, lysozyme, cytochrome C and α-chymotrypsin at pH 7.4 as observed by quantitative normal scanning confocal fluorescence. The surface is also repellent to liposomes (400 nm diameter) of L-α-phosphatidylcholine (Soy-20%). Further possibilities of functionalization on the surface remain available owing to the formation of interfacial SiOH groups.",

author = "L. Ferez and T. Thami and L. Tauk and E. Akpalo and V. Flaud and A. Kocer and J.-M. Janot and P. D{\'e}jardin",

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doi = "10.1021/bk-2012-1120.ch031",

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T1 - Protein-repellent functionalizable surfaces based on covalently bonded phospholipids with phosphorylcholine head

AU - Ferez, L.

AU - Thami, T.

AU - Tauk, L.

AU - Akpalo, E.

AU - Flaud, V.

AU - Kocer, A.

AU - Janot, J.-M.

AU - Déjardin, P.

PY - 2012

Y1 - 2012

N2 - Surface anchored poly(methylhydrosiloxane) (PMHS) films (≈ 10 nm – 1µm) were functionalized with 1,2-dilinoleoyl-sn-glycero-3-phosphorylcholine (18:2 Cis). The surface was characterized by X-ray photoelectron spectroscopy, contact angle measurements, atomic force microscopy, and scanning electron microscopy. The interface was very hydrophilic and repellent toward avidin, bovine serum albumin, bovine fibrinogen, lysozyme, cytochrome C and α-chymotrypsin at pH 7.4 as observed by quantitative normal scanning confocal fluorescence. The surface is also repellent to liposomes (400 nm diameter) of L-α-phosphatidylcholine (Soy-20%). Further possibilities of functionalization on the surface remain available owing to the formation of interfacial SiOH groups.

AB - Surface anchored poly(methylhydrosiloxane) (PMHS) films (≈ 10 nm – 1µm) were functionalized with 1,2-dilinoleoyl-sn-glycero-3-phosphorylcholine (18:2 Cis). The surface was characterized by X-ray photoelectron spectroscopy, contact angle measurements, atomic force microscopy, and scanning electron microscopy. The interface was very hydrophilic and repellent toward avidin, bovine serum albumin, bovine fibrinogen, lysozyme, cytochrome C and α-chymotrypsin at pH 7.4 as observed by quantitative normal scanning confocal fluorescence. The surface is also repellent to liposomes (400 nm diameter) of L-α-phosphatidylcholine (Soy-20%). Further possibilities of functionalization on the surface remain available owing to the formation of interfacial SiOH groups.

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BT - Proteins at Interfaces III State of the Art

ER -

Protein-repellent functionalizable surfaces based on covalently bonded phospholipids with phosphorylcholine head

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