Protein separation using affinity binding. 1. Polystyrene core-shell latex as ligand carrier

B. Gebben, B. Gebben, G.D.B. van Houwelingen, W. Zhang, Anthonie van den Boomgaard, C.A. Smolders, C.A. Smolders

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Abstract

Polystyrene core-shell latex particles are introduced as affinity ligand carriers for affinity separations. The particles, prepared by a seeded emulsion polymerisation process, are submicron in size and composed of a hard polystyrene core surrounded by a hydrophilic shell to which affinity ligands can be covalently coupled. The reactive dye Cibacron Blue is studied as model ligand. This dye ligand, which is covalently coupled directly to hydroxyl groups on the particle surface, is studied and the amount of coupled dye is determined quantitatively by diffuse reflection spectroscopy. The degree of coupling can be controlled by the ionic strength of the reaction medium. The adsorption of bovine serum albumin to the latices appears to be proportional to the ligand density. The functionalised core-shell latices show a high colloidal stability, fast protein adsorption/desorption kinetics and a low non-specific adsorption. The latex particles can find use in affinity separation techniques such as affinity chromatography and affinity membrane filtration.
Original languageUndefined
Pages (from-to)75-84
Number of pages10
JournalColloids and surfaces B: Biointerfaces
Volume1994
Issue number3
DOIs
Publication statusPublished - 1994

Keywords

  • METIS-106897
  • IR-12716

Cite this

Gebben, B., Gebben, B., van Houwelingen, G. D. B., Zhang, W., van den Boomgaard, A., Smolders, C. A., & Smolders, C. A. (1994). Protein separation using affinity binding. 1. Polystyrene core-shell latex as ligand carrier. Colloids and surfaces B: Biointerfaces, 1994(3), 75-84. https://doi.org/10.1016/0927-7765(93)01112-5
Gebben, B. ; Gebben, B. ; van Houwelingen, G.D.B. ; Zhang, W. ; van den Boomgaard, Anthonie ; Smolders, C.A. ; Smolders, C.A. / Protein separation using affinity binding. 1. Polystyrene core-shell latex as ligand carrier. In: Colloids and surfaces B: Biointerfaces. 1994 ; Vol. 1994, No. 3. pp. 75-84.
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title = "Protein separation using affinity binding. 1. Polystyrene core-shell latex as ligand carrier",
abstract = "Polystyrene core-shell latex particles are introduced as affinity ligand carriers for affinity separations. The particles, prepared by a seeded emulsion polymerisation process, are submicron in size and composed of a hard polystyrene core surrounded by a hydrophilic shell to which affinity ligands can be covalently coupled. The reactive dye Cibacron Blue is studied as model ligand. This dye ligand, which is covalently coupled directly to hydroxyl groups on the particle surface, is studied and the amount of coupled dye is determined quantitatively by diffuse reflection spectroscopy. The degree of coupling can be controlled by the ionic strength of the reaction medium. The adsorption of bovine serum albumin to the latices appears to be proportional to the ligand density. The functionalised core-shell latices show a high colloidal stability, fast protein adsorption/desorption kinetics and a low non-specific adsorption. The latex particles can find use in affinity separation techniques such as affinity chromatography and affinity membrane filtration.",
keywords = "METIS-106897, IR-12716",
author = "B. Gebben and B. Gebben and {van Houwelingen}, G.D.B. and W. Zhang and {van den Boomgaard}, Anthonie and C.A. Smolders and C.A. Smolders",
year = "1994",
doi = "10.1016/0927-7765(93)01112-5",
language = "Undefined",
volume = "1994",
pages = "75--84",
journal = "Colloids and surfaces B: Biointerfaces",
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Gebben, B, Gebben, B, van Houwelingen, GDB, Zhang, W, van den Boomgaard, A, Smolders, CA & Smolders, CA 1994, 'Protein separation using affinity binding. 1. Polystyrene core-shell latex as ligand carrier' Colloids and surfaces B: Biointerfaces, vol. 1994, no. 3, pp. 75-84. https://doi.org/10.1016/0927-7765(93)01112-5

Protein separation using affinity binding. 1. Polystyrene core-shell latex as ligand carrier. / Gebben, B.; Gebben, B.; van Houwelingen, G.D.B.; Zhang, W.; van den Boomgaard, Anthonie; Smolders, C.A.; Smolders, C.A.

In: Colloids and surfaces B: Biointerfaces, Vol. 1994, No. 3, 1994, p. 75-84.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Protein separation using affinity binding. 1. Polystyrene core-shell latex as ligand carrier

AU - Gebben, B.

AU - Gebben, B.

AU - van Houwelingen, G.D.B.

AU - Zhang, W.

AU - van den Boomgaard, Anthonie

AU - Smolders, C.A.

AU - Smolders, C.A.

PY - 1994

Y1 - 1994

N2 - Polystyrene core-shell latex particles are introduced as affinity ligand carriers for affinity separations. The particles, prepared by a seeded emulsion polymerisation process, are submicron in size and composed of a hard polystyrene core surrounded by a hydrophilic shell to which affinity ligands can be covalently coupled. The reactive dye Cibacron Blue is studied as model ligand. This dye ligand, which is covalently coupled directly to hydroxyl groups on the particle surface, is studied and the amount of coupled dye is determined quantitatively by diffuse reflection spectroscopy. The degree of coupling can be controlled by the ionic strength of the reaction medium. The adsorption of bovine serum albumin to the latices appears to be proportional to the ligand density. The functionalised core-shell latices show a high colloidal stability, fast protein adsorption/desorption kinetics and a low non-specific adsorption. The latex particles can find use in affinity separation techniques such as affinity chromatography and affinity membrane filtration.

AB - Polystyrene core-shell latex particles are introduced as affinity ligand carriers for affinity separations. The particles, prepared by a seeded emulsion polymerisation process, are submicron in size and composed of a hard polystyrene core surrounded by a hydrophilic shell to which affinity ligands can be covalently coupled. The reactive dye Cibacron Blue is studied as model ligand. This dye ligand, which is covalently coupled directly to hydroxyl groups on the particle surface, is studied and the amount of coupled dye is determined quantitatively by diffuse reflection spectroscopy. The degree of coupling can be controlled by the ionic strength of the reaction medium. The adsorption of bovine serum albumin to the latices appears to be proportional to the ligand density. The functionalised core-shell latices show a high colloidal stability, fast protein adsorption/desorption kinetics and a low non-specific adsorption. The latex particles can find use in affinity separation techniques such as affinity chromatography and affinity membrane filtration.

KW - METIS-106897

KW - IR-12716

U2 - 10.1016/0927-7765(93)01112-5

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JO - Colloids and surfaces B: Biointerfaces

JF - Colloids and surfaces B: Biointerfaces

SN - 0927-7765

IS - 3

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