Proteomic analysis of silica hybrid sol-gel coatings: a potential tool for predicting the biocompatibility of implants in vivo

F. Romero-Gavilan, A. M. Sánchez-Pérez, N. Araújo-Gomes*, M. Azkargorta, I. Iloro, F. Elortza, M. Gurruchaga, I. Goñi, J. Suay

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

The interactions of implanted biomaterials with the host organism determine the success or failure of an implantation. Normally, their biocompatibility is assessed using in vitro tests. Unfortunately, in vitro and in vivo results are not always concordant; new, effective methods of biomaterial characterisation are urgently needed to predict the in vivo outcome. As the first layer of proteins adsorbed onto the biomaterial surfaces might condition the host response, mass spectrometry analysis was performed to characterise these proteins. Four distinct hybrid sol-gel biomaterials were tested. The in vitro results were similar for all the materials examined here. However, in vivo, the materials behaved differently. Six of the 171 adsorbed proteins were significantly more abundant on the materials with weak biocompatibility; these proteins are associated with the complement pathway. Thus, protein analysis might be a suitable tool to predict the in vivo outcomes of implantations using newly formulated biomaterials.

Original languageEnglish
Pages (from-to)676-689
Number of pages14
JournalBiofouling
Volume33
Issue number8
DOIs
Publication statusPublished - 14 Sep 2017
Externally publishedYes

Keywords

  • bone regeneration
  • C-reactive protein
  • dental implants
  • fibrous capsule
  • Haemocompatibility
  • osteoimmunology

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