Pulling angle-dependent force microscopy

Lucie Grebíková, Hubert Gojzewski, B.D. Kieviet, Michel Klein Gunnewiek, G.J. Vancso

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

In this paper, we describe a method allowing one to perform three-dimensional displacement control in force spectroscopy by atomic force microscopy (AFM). Traditionally, AFM force curves are measured in the normal direction of the contacted surface. The method described can be employed to address not only the magnitude of the measured force but also its direction. We demonstrate the technique using a case study of angle-dependent desorption of a single poly(2-hydroxyethyl methacrylate) (PHEMA) chain from a planar silica surface in an aqueous solution. The chains were end-grafted from the AFM tip in high dilution, enabling single macromolecule pull experiments. Our experiments give evidence of angular dependence of the desorption force of single polymer chains and illustrate the added value of introducing force direction control in AFM.
Original languageEnglish
Article number033705
Number of pages7
JournalReview of scientific instruments
Volume88
Issue number3
DOIs
Publication statusPublished - 30 Mar 2017

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pulling
Atomic force microscopy
Microscopic examination
microscopy
atomic force microscopy
Desorption
Displacement control
desorption
Macromolecules
Dilution
macromolecules
Experiments
Silica
Spectroscopy
dilution
silicon dioxide
aqueous solutions
Polymers
polymers
curves

Cite this

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title = "Pulling angle-dependent force microscopy",
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Pulling angle-dependent force microscopy. / Grebíková, Lucie ; Gojzewski, Hubert ; Kieviet, B.D.; Klein Gunnewiek, Michel ; Vancso, G.J.

In: Review of scientific instruments, Vol. 88, No. 3, 033705, 30.03.2017.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Grebíková, Lucie

AU - Gojzewski, Hubert

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AU - Klein Gunnewiek, Michel

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