Quantitative analysis of single muscle fibre action potentials recorded at known distances

B.A. Albers, J.H.M. Put, W. Wallinga, P. Wirtz

    Research output: Contribution to journalArticleAcademic

    13 Citations (Scopus)
    53 Downloads (Pure)

    Abstract

    In vivo records of single fibre action potentials (SFAPs) have always been obtained at unknown distance from the active muscle fibre. A new experimental method has been developed enabling the derivation of the recording distance in animal experiments. A single fibre is stimulated with an intracellular micropipette electrode. The same electrode is used thereafter for labelling with an auto-fluorescent dye, Lucifer Yellow. In this method there is no use of chemical fixation. The tissue structure is kept as well as possible. In cross-sections the fluorescent fibre is seen and its position is quantitized with respect to the tip of one or more recording wire electrodes. Morphometric data, such as the recording distance and the fibre cross-sectional area, are used for the interpretation of parameters of the SFAPs (peak-peak amplitude, time between the first positive and negative peaks). The present results show that within 300 μm recording distance is not as dominant for the SFAP shape as expected. The method offers also a direct check of the relation between the muscle fibre; diameter and the conduction velocity of the action potential. In the present small set of data there is no simple linear relationship.
    Original languageUndefined
    Pages (from-to)245-253
    JournalElectroencephalography and clinical neurophysiology
    Volume73
    Issue number3
    DOIs
    Publication statusPublished - 1989

    Keywords

    • Muscle fibre
    • IR-70572
    • Action potential
    • Conduction velocity
    • Recording distance
    • Fibre cross-section
    • Fibre labelling
    • SFEMG

    Cite this

    Albers, B.A. ; Put, J.H.M. ; Wallinga, W. ; Wirtz, P. / Quantitative analysis of single muscle fibre action potentials recorded at known distances. In: Electroencephalography and clinical neurophysiology. 1989 ; Vol. 73, No. 3. pp. 245-253.
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    title = "Quantitative analysis of single muscle fibre action potentials recorded at known distances",
    abstract = "In vivo records of single fibre action potentials (SFAPs) have always been obtained at unknown distance from the active muscle fibre. A new experimental method has been developed enabling the derivation of the recording distance in animal experiments. A single fibre is stimulated with an intracellular micropipette electrode. The same electrode is used thereafter for labelling with an auto-fluorescent dye, Lucifer Yellow. In this method there is no use of chemical fixation. The tissue structure is kept as well as possible. In cross-sections the fluorescent fibre is seen and its position is quantitized with respect to the tip of one or more recording wire electrodes. Morphometric data, such as the recording distance and the fibre cross-sectional area, are used for the interpretation of parameters of the SFAPs (peak-peak amplitude, time between the first positive and negative peaks). The present results show that within 300 μm recording distance is not as dominant for the SFAP shape as expected. The method offers also a direct check of the relation between the muscle fibre; diameter and the conduction velocity of the action potential. In the present small set of data there is no simple linear relationship.",
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    author = "B.A. Albers and J.H.M. Put and W. Wallinga and P. Wirtz",
    year = "1989",
    doi = "10.1016/0013-4694(89)90125-9",
    language = "Undefined",
    volume = "73",
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    Quantitative analysis of single muscle fibre action potentials recorded at known distances. / Albers, B.A.; Put, J.H.M.; Wallinga, W.; Wirtz, P.

    In: Electroencephalography and clinical neurophysiology, Vol. 73, No. 3, 1989, p. 245-253.

    Research output: Contribution to journalArticleAcademic

    TY - JOUR

    T1 - Quantitative analysis of single muscle fibre action potentials recorded at known distances

    AU - Albers, B.A.

    AU - Put, J.H.M.

    AU - Wallinga, W.

    AU - Wirtz, P.

    PY - 1989

    Y1 - 1989

    N2 - In vivo records of single fibre action potentials (SFAPs) have always been obtained at unknown distance from the active muscle fibre. A new experimental method has been developed enabling the derivation of the recording distance in animal experiments. A single fibre is stimulated with an intracellular micropipette electrode. The same electrode is used thereafter for labelling with an auto-fluorescent dye, Lucifer Yellow. In this method there is no use of chemical fixation. The tissue structure is kept as well as possible. In cross-sections the fluorescent fibre is seen and its position is quantitized with respect to the tip of one or more recording wire electrodes. Morphometric data, such as the recording distance and the fibre cross-sectional area, are used for the interpretation of parameters of the SFAPs (peak-peak amplitude, time between the first positive and negative peaks). The present results show that within 300 μm recording distance is not as dominant for the SFAP shape as expected. The method offers also a direct check of the relation between the muscle fibre; diameter and the conduction velocity of the action potential. In the present small set of data there is no simple linear relationship.

    AB - In vivo records of single fibre action potentials (SFAPs) have always been obtained at unknown distance from the active muscle fibre. A new experimental method has been developed enabling the derivation of the recording distance in animal experiments. A single fibre is stimulated with an intracellular micropipette electrode. The same electrode is used thereafter for labelling with an auto-fluorescent dye, Lucifer Yellow. In this method there is no use of chemical fixation. The tissue structure is kept as well as possible. In cross-sections the fluorescent fibre is seen and its position is quantitized with respect to the tip of one or more recording wire electrodes. Morphometric data, such as the recording distance and the fibre cross-sectional area, are used for the interpretation of parameters of the SFAPs (peak-peak amplitude, time between the first positive and negative peaks). The present results show that within 300 μm recording distance is not as dominant for the SFAP shape as expected. The method offers also a direct check of the relation between the muscle fibre; diameter and the conduction velocity of the action potential. In the present small set of data there is no simple linear relationship.

    KW - Muscle fibre

    KW - IR-70572

    KW - Action potential

    KW - Conduction velocity

    KW - Recording distance

    KW - Fibre cross-section

    KW - Fibre labelling

    KW - SFEMG

    U2 - 10.1016/0013-4694(89)90125-9

    DO - 10.1016/0013-4694(89)90125-9

    M3 - Article

    VL - 73

    SP - 245

    EP - 253

    JO - Electroencephalography and clinical neurophysiology

    JF - Electroencephalography and clinical neurophysiology

    SN - 0013-4694

    IS - 3

    ER -