Quantitative analysis of single muscle fibre action potentials recorded at known distances

B.A. Albers, J.H.M. Put, W. Wallinga, P. Wirtz

Research output: Contribution to journalArticleAcademic

11 Citations (Scopus)
48 Downloads (Pure)

Abstract

In vivo records of single fibre action potentials (SFAPs) have always been obtained at unknown distance from the active muscle fibre. A new experimental method has been developed enabling the derivation of the recording distance in animal experiments. A single fibre is stimulated with an intracellular micropipette electrode. The same electrode is used thereafter for labelling with an auto-fluorescent dye, Lucifer Yellow. In this method there is no use of chemical fixation. The tissue structure is kept as well as possible. In cross-sections the fluorescent fibre is seen and its position is quantitized with respect to the tip of one or more recording wire electrodes. Morphometric data, such as the recording distance and the fibre cross-sectional area, are used for the interpretation of parameters of the SFAPs (peak-peak amplitude, time between the first positive and negative peaks). The present results show that within 300 μm recording distance is not as dominant for the SFAP shape as expected. The method offers also a direct check of the relation between the muscle fibre; diameter and the conduction velocity of the action potential. In the present small set of data there is no simple linear relationship.
Original languageUndefined
Pages (from-to)245-253
JournalElectroencephalography and clinical neurophysiology
Volume73
Issue number3
DOIs
Publication statusPublished - 1989

Keywords

  • Muscle fibre
  • IR-70572
  • Action potential
  • Conduction velocity
  • Recording distance
  • Fibre cross-section
  • Fibre labelling
  • SFEMG

Cite this

Albers, B.A. ; Put, J.H.M. ; Wallinga, W. ; Wirtz, P. / Quantitative analysis of single muscle fibre action potentials recorded at known distances. In: Electroencephalography and clinical neurophysiology. 1989 ; Vol. 73, No. 3. pp. 245-253.
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abstract = "In vivo records of single fibre action potentials (SFAPs) have always been obtained at unknown distance from the active muscle fibre. A new experimental method has been developed enabling the derivation of the recording distance in animal experiments. A single fibre is stimulated with an intracellular micropipette electrode. The same electrode is used thereafter for labelling with an auto-fluorescent dye, Lucifer Yellow. In this method there is no use of chemical fixation. The tissue structure is kept as well as possible. In cross-sections the fluorescent fibre is seen and its position is quantitized with respect to the tip of one or more recording wire electrodes. Morphometric data, such as the recording distance and the fibre cross-sectional area, are used for the interpretation of parameters of the SFAPs (peak-peak amplitude, time between the first positive and negative peaks). The present results show that within 300 μm recording distance is not as dominant for the SFAP shape as expected. The method offers also a direct check of the relation between the muscle fibre; diameter and the conduction velocity of the action potential. In the present small set of data there is no simple linear relationship.",
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Quantitative analysis of single muscle fibre action potentials recorded at known distances. / Albers, B.A.; Put, J.H.M.; Wallinga, W.; Wirtz, P.

In: Electroencephalography and clinical neurophysiology, Vol. 73, No. 3, 1989, p. 245-253.

Research output: Contribution to journalArticleAcademic

TY - JOUR

T1 - Quantitative analysis of single muscle fibre action potentials recorded at known distances

AU - Albers, B.A.

AU - Put, J.H.M.

AU - Wallinga, W.

AU - Wirtz, P.

PY - 1989

Y1 - 1989

N2 - In vivo records of single fibre action potentials (SFAPs) have always been obtained at unknown distance from the active muscle fibre. A new experimental method has been developed enabling the derivation of the recording distance in animal experiments. A single fibre is stimulated with an intracellular micropipette electrode. The same electrode is used thereafter for labelling with an auto-fluorescent dye, Lucifer Yellow. In this method there is no use of chemical fixation. The tissue structure is kept as well as possible. In cross-sections the fluorescent fibre is seen and its position is quantitized with respect to the tip of one or more recording wire electrodes. Morphometric data, such as the recording distance and the fibre cross-sectional area, are used for the interpretation of parameters of the SFAPs (peak-peak amplitude, time between the first positive and negative peaks). The present results show that within 300 μm recording distance is not as dominant for the SFAP shape as expected. The method offers also a direct check of the relation between the muscle fibre; diameter and the conduction velocity of the action potential. In the present small set of data there is no simple linear relationship.

AB - In vivo records of single fibre action potentials (SFAPs) have always been obtained at unknown distance from the active muscle fibre. A new experimental method has been developed enabling the derivation of the recording distance in animal experiments. A single fibre is stimulated with an intracellular micropipette electrode. The same electrode is used thereafter for labelling with an auto-fluorescent dye, Lucifer Yellow. In this method there is no use of chemical fixation. The tissue structure is kept as well as possible. In cross-sections the fluorescent fibre is seen and its position is quantitized with respect to the tip of one or more recording wire electrodes. Morphometric data, such as the recording distance and the fibre cross-sectional area, are used for the interpretation of parameters of the SFAPs (peak-peak amplitude, time between the first positive and negative peaks). The present results show that within 300 μm recording distance is not as dominant for the SFAP shape as expected. The method offers also a direct check of the relation between the muscle fibre; diameter and the conduction velocity of the action potential. In the present small set of data there is no simple linear relationship.

KW - Muscle fibre

KW - IR-70572

KW - Action potential

KW - Conduction velocity

KW - Recording distance

KW - Fibre cross-section

KW - Fibre labelling

KW - SFEMG

U2 - 10.1016/0013-4694(89)90125-9

DO - 10.1016/0013-4694(89)90125-9

M3 - Article

VL - 73

SP - 245

EP - 253

JO - Electroencephalography and clinical neurophysiology

JF - Electroencephalography and clinical neurophysiology

SN - 0013-4694

IS - 3

ER -