Quantitative analysis of thiols in consumer products on a microfluidic CE chip with fluorescence detection

T. Revermann, S. Götz, U. Karst

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    11 Citations (Scopus)


    A microchip CE-based method for the quantification of the thiols mercaptoethanoic acid (MAA) and 2-mercaptopropionic acid (2-MPA) in depilatory cream and cold wave lotions was developed. The thiols were first derivatized with the fluorogenic reagent ammonium-7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD-F). The derivatives were separated within only 20 s by microchip CE and detected by their fluorescence. Conventional CE with diode array detection and LC with fluorescence detection were used for validation. The internal standard 3-mercaptopropionic acid (3-MPA) provided RSDs of multiple injections of only 4% or less for the MCE approach. LOD is 2 µM, LOQ 6 µM, and the linear range comprises nearly three decades of concentration starting at the LOQ.
    Original languageUndefined
    Pages (from-to)1154-1160
    Issue number7
    Publication statusPublished - 2007


    • Quantitative analysis
    • Microchip CE
    • Derivatization
    • Fluorescence detection
    • IR-72255
    • Thiols
    • METIS-246554

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