Quantitative blood oxygen saturation imaging using combined photoacoustics and acousto-optics

Altaf Hussain, Wilhelmina Petersen, J.W. Staley, Erwin Hondebrink, Wiendelt Steenbergen

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41 Citations (Scopus)
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In photoacoustic spectroscopy (PAS), wavelength dependent optical attenuation of biological tissue presents a challenge to measure the absolute oxygen saturation of hemoglobin (sO 2). Here, we employ the combination of photoacoustics and acousto-optics (AO) at two optical wavelengths to achieve quantification, where AO serves as a sensor for the relative local fluence. We demonstrate that our method enables compensation of spatial as well as wavelength dependent fluence variations in PAS without a priori knowledge about the optical properties of the medium. The fluence compensated photoacoustic images at two excitation wavelengths are used to estimate the absolute oxygen saturation of blood in a spatially and spectroscopically heterogeneous phantom.
Original languageEnglish
Pages (from-to)1720-1723
Number of pages4
JournalOptics letters
Issue number8
Publication statusPublished - 2016


  • METIS-316337
  • IR-101158


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