Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Gruneisen parameter measurements: Demonstration with human blood

Y.Y. Villanueva, Erwin Hondebrink, Wilhelmina Petersen, Wiendelt Steenbergen

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Abstract

Quantitative photoacoustic imaging in biomedicine relies on accurate measurements of relevant material properties of target absorbers. Here, we present a method for simultaneous measurements of the absorption coefficient and Grüneisen parameter of small volume of liquid scattering and absorbing media using a coupled-integrating sphere system which we refer to as quantitative photoacoustic integrating sphere (QPAIS) platform. The derived equations do not require absolute magnitudes of optical energy and pressure values, only calibration of the setup using aqueous ink dilutions is necessary. As a demonstration, measurements with blood samples from various human donors are done at room and body temperatures using an incubator. Measured absorption coefficient values are consistent with known oxygen saturation dependence of blood absorption at 750 nm, whereas measured Grüneisen parameter values indicate variability among five different donors. An increasing Grüneisen parameter value with both hematocrit and temperature is observed. These observations are consistent with those reported in literature.
Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalPhotoacoustics
Volume6
DOIs
Publication statusPublished - 2017

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blood
absorptivity
platforms
hematocrit
body temperature
Incubators
Ink
inks
Body Temperature
Hematocrit
Calibration
dilution
absorbers
Oxygen
saturation
Pressure
Temperature
room temperature
oxygen
liquids

Keywords

  • METIS-322064
  • IR-104630

Cite this

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title = "Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Gruneisen parameter measurements: Demonstration with human blood",
abstract = "Quantitative photoacoustic imaging in biomedicine relies on accurate measurements of relevant material properties of target absorbers. Here, we present a method for simultaneous measurements of the absorption coefficient and Gr{\"u}neisen parameter of small volume of liquid scattering and absorbing media using a coupled-integrating sphere system which we refer to as quantitative photoacoustic integrating sphere (QPAIS) platform. The derived equations do not require absolute magnitudes of optical energy and pressure values, only calibration of the setup using aqueous ink dilutions is necessary. As a demonstration, measurements with blood samples from various human donors are done at room and body temperatures using an incubator. Measured absorption coefficient values are consistent with known oxygen saturation dependence of blood absorption at 750 nm, whereas measured Gr{\"u}neisen parameter values indicate variability among five different donors. An increasing Gr{\"u}neisen parameter value with both hematocrit and temperature is observed. These observations are consistent with those reported in literature.",
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author = "Y.Y. Villanueva and Erwin Hondebrink and Wilhelmina Petersen and Wiendelt Steenbergen",
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doi = "10.1016/j.pacs.2017.03.004",
language = "English",
volume = "6",
pages = "9--15",
journal = "Photoacoustics",
issn = "2213-5979",
publisher = "Elsevier",

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Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Gruneisen parameter measurements : Demonstration with human blood. / Villanueva, Y.Y.; Hondebrink, Erwin; Petersen, Wilhelmina; Steenbergen, Wiendelt.

In: Photoacoustics, Vol. 6, 2017, p. 9-15.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Gruneisen parameter measurements

T2 - Demonstration with human blood

AU - Villanueva, Y.Y.

AU - Hondebrink, Erwin

AU - Petersen, Wilhelmina

AU - Steenbergen, Wiendelt

PY - 2017

Y1 - 2017

N2 - Quantitative photoacoustic imaging in biomedicine relies on accurate measurements of relevant material properties of target absorbers. Here, we present a method for simultaneous measurements of the absorption coefficient and Grüneisen parameter of small volume of liquid scattering and absorbing media using a coupled-integrating sphere system which we refer to as quantitative photoacoustic integrating sphere (QPAIS) platform. The derived equations do not require absolute magnitudes of optical energy and pressure values, only calibration of the setup using aqueous ink dilutions is necessary. As a demonstration, measurements with blood samples from various human donors are done at room and body temperatures using an incubator. Measured absorption coefficient values are consistent with known oxygen saturation dependence of blood absorption at 750 nm, whereas measured Grüneisen parameter values indicate variability among five different donors. An increasing Grüneisen parameter value with both hematocrit and temperature is observed. These observations are consistent with those reported in literature.

AB - Quantitative photoacoustic imaging in biomedicine relies on accurate measurements of relevant material properties of target absorbers. Here, we present a method for simultaneous measurements of the absorption coefficient and Grüneisen parameter of small volume of liquid scattering and absorbing media using a coupled-integrating sphere system which we refer to as quantitative photoacoustic integrating sphere (QPAIS) platform. The derived equations do not require absolute magnitudes of optical energy and pressure values, only calibration of the setup using aqueous ink dilutions is necessary. As a demonstration, measurements with blood samples from various human donors are done at room and body temperatures using an incubator. Measured absorption coefficient values are consistent with known oxygen saturation dependence of blood absorption at 750 nm, whereas measured Grüneisen parameter values indicate variability among five different donors. An increasing Grüneisen parameter value with both hematocrit and temperature is observed. These observations are consistent with those reported in literature.

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KW - IR-104630

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